##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632032.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322461
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.612170153910085	34.0	31.0	34.0	31.0	34.0
2	32.772009638374875	34.0	31.0	34.0	31.0	34.0
3	32.78357692868285	34.0	31.0	34.0	31.0	34.0
4	36.29164457097137	37.0	37.0	37.0	35.0	37.0
5	36.183699734231425	37.0	35.0	37.0	35.0	37.0
6	36.2206499390624	37.0	36.0	37.0	35.0	37.0
7	36.20951370863453	37.0	36.0	37.0	35.0	37.0
8	36.184366481528	37.0	36.0	37.0	35.0	37.0
9	37.956903315439696	39.0	38.0	39.0	35.0	39.0
10	37.875646977463944	39.0	38.0	39.0	35.0	39.0
11	37.988845162670835	39.0	38.0	39.0	35.0	39.0
12	37.948009216618445	39.0	38.0	39.0	35.0	39.0
13	37.965791211960514	39.0	38.0	39.0	35.0	39.0
14	39.33738343551623	40.0	39.0	41.0	37.0	41.0
15	39.364989874744545	40.0	39.0	41.0	37.0	41.0
16	39.27109324848586	40.0	39.0	41.0	36.0	41.0
17	39.310387922880594	40.0	39.0	41.0	36.0	41.0
18	39.316500289957546	40.0	39.0	41.0	36.0	41.0
19	39.370785924499394	40.0	39.0	41.0	37.0	41.0
20	39.36228877290587	40.0	39.0	41.0	37.0	41.0
21	39.335392497077166	40.0	39.0	41.0	36.0	41.0
22	39.32326389857998	40.0	39.0	41.0	36.0	41.0
23	39.26958608948059	40.0	39.0	41.0	36.0	41.0
24	39.217768970511166	40.0	39.0	41.0	36.0	41.0
25	39.16747761744831	40.0	39.0	41.0	36.0	41.0
26	39.08751135796267	40.0	39.0	41.0	36.0	41.0
27	39.01934187390103	40.0	39.0	41.0	36.0	41.0
28	38.95799492031595	40.0	39.0	41.0	35.0	41.0
29	38.841469200926625	40.0	38.0	41.0	35.0	41.0
30	38.790213390146405	40.0	38.0	41.0	35.0	41.0
31	38.73113027621945	40.0	38.0	41.0	35.0	41.0
32	38.654153525542625	40.0	38.0	41.0	35.0	41.0
33	38.54183916814747	40.0	38.0	41.0	35.0	41.0
34	38.554101116103965	40.0	38.0	41.0	35.0	41.0
35	38.512133250222504	40.0	38.0	41.0	35.0	41.0
36	38.5065697867339	40.0	38.0	41.0	35.0	41.0
37	38.49527539764499	40.0	38.0	41.0	35.0	41.0
38	38.43563407667904	40.0	38.0	41.0	34.0	41.0
39	38.28321254353239	40.0	38.0	41.0	34.0	41.0
40	38.2962404755924	40.0	38.0	41.0	34.0	41.0
41	38.31076316205681	40.0	38.0	41.0	34.0	41.0
42	38.21678280474228	40.0	38.0	41.0	34.0	41.0
43	37.36430762169689	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	2.0
18	10.0
19	16.0
20	23.0
21	42.0
22	85.0
23	155.0
24	235.0
25	357.0
26	499.0
27	747.0
28	1116.0
29	1520.0
30	2144.0
31	3063.0
32	4140.0
33	5641.0
34	8165.0
35	12359.0
36	20004.0
37	35778.0
38	86466.0
39	139890.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.56421706811056	17.79936178328542	12.702311287256443	26.934109861347572
2	18.725365237966763	20.167400088692897	34.52262444140532	26.584610231935024
3	19.799293557980654	20.969357534709623	30.140389070306178	29.090959837003545
4	15.40620416112336	15.176098815050501	33.46637267762613	35.9513243462
5	15.871996923658985	34.877706141207774	33.786721495002496	15.463575440130745
6	36.222054760110524	33.858668180027976	15.083994653617028	14.835282406244477
7	30.949169046799458	29.567606625297323	20.082428572757635	19.400795755145584
8	27.97950759936861	33.27782274445592	19.572909592167733	19.16976006400774
9	27.514335066876306	14.001072997974948	18.301748118377105	40.18284381677164
10	17.349695001876196	26.331246259237552	32.29940985111378	24.019648887772473
11	37.36296792480331	20.658622283004764	20.716923907077135	21.26148588511479
12	23.206837415997594	24.08570338738638	28.04711267409082	24.660346522525202
13	32.11148014798689	18.242826264261414	23.3857737834963	26.2599198042554
14	23.836060794948846	19.206043521542142	23.72783065238897	33.23006503112004
15	26.47731043444013	26.08687562216826	21.11542170991221	26.320392233479396
16	26.49839825591312	24.89634405400963	22.382861803442896	26.222395886634352
17	25.31810048346932	25.068768006053443	23.994529571017893	25.618601939459346
18	24.98720775535646	24.041977169332103	25.101019968306243	25.86979510700519
19	26.30271567724469	23.94181001733543	24.874946117514988	24.880528187904893
20	26.623994839686038	23.56036854069174	24.740046083092217	25.07559053653
21	26.80479189731471	24.248203658737026	23.110391644260854	25.8366127996874
22	26.893794908531575	23.829548379493954	23.3132068684275	25.96344984354697
23	26.125329884854292	24.15392869215192	23.583317052294696	26.13742437069909
24	26.657487262025487	23.736823988017157	23.666737993121647	25.93895075683571
25	26.412806509934533	23.2124194863875	23.99483968603955	26.37993431763841
26	25.99601192082143	23.48873197068793	23.892191613869585	26.623064494621058
27	25.929647306185867	23.661466037753403	23.885989313436355	26.52289734262438
28	25.66915068799018	23.841952980360414	24.052210965046935	26.436685366602475
29	25.43098235135412	24.482340500091485	24.33937747510552	25.747299673448882
30	25.340118650007287	24.999922471244584	23.726900307323987	25.93305857142414
31	25.925925925925924	24.217812386614195	23.888470233609645	25.967791453850232
32	24.636467665857268	24.432411981603977	24.644840771442126	26.28627958109663
33	25.208629880822798	23.773727675594877	24.758032754348587	26.259609689233738
34	25.5568890501487	23.263588464961654	25.049850989732093	26.129671495157552
35	25.02163052276089	23.43074046163722	25.30259473238624	26.245034283215645
36	24.96425924375351	23.186679939589595	25.47998052477664	26.36908029188026
37	25.12086732969258	21.952732268398346	25.74823001851387	27.1781703833952
38	24.127258800289027	22.297890287507638	26.880459962600128	26.694390949603207
39	24.22711583726404	21.66618598838309	27.788476746025097	26.318221428327764
40	23.498965766402762	21.311104288580633	28.903960478941638	26.285969466074967
41	22.53171701384043	21.2791624413495	29.79957266150015	26.389547883309923
42	22.53543839410037	20.84903290630495	29.889195902760335	26.726332796834345
43	21.328160614772017	20.724676782618673	30.532064342664693	27.415098259944614
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	4.5
2	4.0
3	15.0
4	26.0
5	26.0
6	34.5
7	43.0
8	42.0
9	41.0
10	63.0
11	85.0
12	85.0
13	150.5
14	216.0
15	406.0
16	596.0
17	632.0
18	668.0
19	668.0
20	806.0
21	944.0
22	1189.0
23	1434.0
24	1793.5
25	2153.0
26	2153.0
27	2615.0
28	3077.0
29	4091.5
30	5106.0
31	6073.0
32	7040.0
33	7040.0
34	8165.5
35	9291.0
36	10342.5
37	11394.0
38	13060.0
39	14726.0
40	14726.0
41	16332.5
42	17939.0
43	18369.5
44	18800.0
45	19861.5
46	20923.0
47	20923.0
48	22040.0
49	23157.0
50	24594.0
51	26031.0
52	27450.5
53	28870.0
54	28870.0
55	27013.0
56	25156.0
57	24041.0
58	22926.0
59	21327.0
60	19728.0
61	19728.0
62	18329.0
63	16930.0
64	15336.5
65	13743.0
66	11929.5
67	10116.0
68	10116.0
69	8699.5
70	7283.0
71	6330.0
72	5377.0
73	4386.0
74	3395.0
75	3395.0
76	2763.5
77	2132.0
78	1766.5
79	1401.0
80	1094.5
81	788.0
82	788.0
83	637.5
84	487.0
85	365.5
86	244.0
87	178.5
88	113.0
89	113.0
90	81.0
91	49.0
92	31.0
93	13.0
94	10.0
95	7.0
96	7.0
97	5.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	322461.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	78.20073751131108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.66001616594257	72.46081601986715
2	4.365429524440718	6.8275961672983225
3	1.0725639747435665	2.516258815590304
4	0.4940678680137526	1.545458866372662
5	0.30472478872778236	1.1914851608245518
6	0.20736971285407405	0.9729878689618436
7	0.12299162911156844	0.6732625272969603
8	0.09386543485830538	0.5872276986191527
9	0.07724760790761723	0.5436737918424228
>10	0.5367803354566812	8.095549247895441
>50	0.046615497419534434	2.4666978478841246
>100	0.01832746052385124	2.118985987547174
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	361	0.11195152281981387	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	6.202300433230685E-4	0.0
18	0.0	0.0	0.0	6.202300433230685E-4	0.0
19	0.0	0.0	0.0	9.303450649846028E-4	0.0
20	0.0	0.0	0.0	0.001240460086646137	0.0
21	0.0	0.0	0.0	0.0018606901299692057	0.0
22	0.0	0.0	0.0	0.0018606901299692057	0.0
23	0.0	0.0	0.0	0.004341610303261479	0.0
24	0.0	0.0	0.0	0.005271955368246082	0.0
25	0.0	0.0	0.0	0.005892185411569151	0.0
26	0.0	0.0	0.0	0.008373105584861425	0.0
27	0.0	0.0	0.0	0.028220466971199617	0.0
28	0.0	0.0	0.0	0.11164140779815233	0.0
29	0.0	0.0	0.0	0.24282006196098133	0.0
30	0.0	0.0	0.0	0.4078012534849176	0.0
31	0.0	0.0	0.0	0.9973299096634942	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATTGA	25	0.005490553	29.6	1
GGTATCA	150	0.0	27.133333	1
TCTATAC	35	8.855507E-4	26.42857	3
CTATACT	45	1.3203299E-4	24.666666	4
TAGACAG	40	0.0019281917	23.125	5
CCGGACT	40	0.0019281917	23.125	4
CTTATAC	345	0.0	21.449274	37
CGGCCTT	95	7.0976967E-9	21.421053	24
TATACTT	45	0.0038199304	20.555555	5
ATACACA	65	6.882465E-5	19.923077	37
GTATCAA	280	0.0	19.160713	1
TAGAGAG	50	0.007024178	18.5	5
TGGACAT	60	9.217182E-4	18.5	5
TCCTAAC	60	9.217182E-4	18.5	2
CCCCGTC	60	9.217182E-4	18.5	9
CCATTTG	70	1.21574885E-4	18.5	9
TCTTATA	635	0.0	17.480314	37
CTTTGAG	65	0.0015766032	17.076923	2
TTCCTCG	130	1.38225005E-8	17.076923	19
TCGCCAT	80	3.3731342E-4	16.1875	13
>>END_MODULE