FastQCFastQC Report
Fri 10 Feb 2017
ERR1632031.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632031.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331269
Sequences flagged as poor quality0
Sequence length43
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42141.272077978923473No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33571.0133758365557899No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29250.8829682222000852No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11870.3583190700005132No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT10040.3030769555859437No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT9330.28164422267100153RNA PCR Primer, Index 29 (95% over 23bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5330.16089643160090442No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.11591787942729322No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3720.11229544569519032No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG3330.10052253606585583No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA7200.031.3472211
CCGTCTT1200.024.66666837
GCCGTCT1200.024.66666836
GACTGTA502.6964795E-422.27
TATGCCG1400.021.14285933
CTATAGG450.00382014820.5555554
GCAGACA555.1324803E-420.18181837
ATGCCGT1551.8189894E-1219.09677534
TGCCGTC1551.8189894E-1219.09677535
AGTACAC500.007024572718.55
CGTATGC1653.6379788E-1217.93939431
GTATCAA12600.017.9126992
TCGTATG1851.8189894E-1116.030
GTATGCC1954.1836756E-1115.17948732
CTCGTAT2006.184564E-1114.829
ATTCTAG750.00409621114.81
TCTCGTA2059.094947E-1114.43902428
TTGAGCT908.2521176E-414.3888884
ATCTCCG2500.014.06000110
TATCAAC20250.013.7037041
Produced by FastQC (version 0.11.5)