FastQCFastQC Report
Fri 10 Feb 2017
ERR1632030.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632030.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6495641
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25750.026.1514571
CTTATAC49850.018.40722337
GTATCAA42850.015.6289382
TCTTATA85200.015.52523437
CGAATTA4200.015.41666615
TAGGACA21000.013.6547614
GCTTAGG17150.013.3760931
TTAGGAC23250.012.7311823
CTCTTAT129500.012.65714337
GTATTAG12400.012.5322581
GTATTAA12800.012.4296881
TTAGAGT7350.012.3333344
ATCAACG63300.012.3333332
CGCGCTA9800.012.27040924
CTTAGGA18950.012.2031662
ATACCGT3950.012.1772156
CAACGCA67300.012.01262954
TACCGTC3700.012.07
TCAACGC65950.011.9780133
CTAGTAC2502.1518645E-911.843
Produced by FastQC (version 0.11.5)