FastQCFastQC Report
Fri 10 Feb 2017
ERR1632029.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632029.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117387
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT1850.1575983711995366TruSeq Adapter, Index 6 (95% over 21bp)
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC1620.13800506018554012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG200.001835210437.05
TACACAG250.00547650429.5999985
CTTATAC1254.0017767E-1019.2437
ACTTTGG609.169622E-418.58
GCTGTGC609.169622E-418.58
GCTGCAT500.006998223318.49999812
TATACAC500.006998223318.49999836
CGCCCTC500.006998223318.49999814
GGTCTGT650.001568534417.07692333
GTCTGTC803.3503078E-416.187534
CAGGACA855.3133623E-415.2352944
GTATCAA750.00407527814.81
TCTTATA2151.2732926E-1114.62790837
ATCAACG908.196241E-414.3888882
TCAACGC908.196241E-414.3888883
TAGTTGC800.00625429413.87527
TATCAAC1000.001815504912.9499991
TGCAGGA1153.534724E-412.8695652
TGTCTCT9250.012.637
TCTGTCT2351.0451913E-811.80851135
Produced by FastQC (version 0.11.5)