FastQCFastQC Report
Fri 10 Feb 2017
ERR1632028.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632028.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12689
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT3452.718890377492316Illumina PCR Primer Index 10 (95% over 21bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC1761.3870281346047757No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT1601.2609346678225235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1571.237292142800851No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1511.1900070927575066No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC1230.969343525888565No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1120.8826542674757665No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC950.7486799590196233Illumina PCR Primer Index 10 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG910.7171565923240603No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT440.3467570336511939No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG400.3152336669556309No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220.17337851682559696No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.11033178343447081No Hit
ATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT130.10245094176058002No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT603.6585334E-827.75000237
GCCGTCT603.6585334E-827.75000236
GGTATCA1000.025.9000021
GTATCAA1251.2732926E-1120.722
ATGCCGT901.8315768E-618.534
TGCCGTC953.0719002E-617.52631635
TATGCCG1005.0112194E-616.65000233
AATCTCG1201.5952701E-615.41666726
TCTCGTA1101.2399909E-515.13636428
CGTATGC1101.2399909E-515.13636431
TCGTATG1101.2399909E-515.13636430
TCTCCGA1101.2399909E-515.1363641
GAGTACT750.00381393314.813
GTATGCC1151.8890607E-514.47826132
ATCTCGT1151.8890607E-514.47826127
TCCGAGC1151.8890607E-514.4782613
CTCGTAT1151.8890607E-514.47826129
CTCCGAG1151.8890607E-514.4782612
ATCAACG1802.3064786E-914.3888894
TATCAAC1853.3978722E-914.03
Produced by FastQC (version 0.11.5)