##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632028.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12689
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.03640948853337	33.0	31.0	34.0	30.0	34.0
2	32.19063756009142	33.0	31.0	34.0	30.0	34.0
3	32.124438490030734	34.0	31.0	34.0	30.0	34.0
4	35.802663724485775	37.0	35.0	37.0	35.0	37.0
5	35.63858460083537	37.0	35.0	37.0	33.0	37.0
6	35.700291591141934	37.0	35.0	37.0	33.0	37.0
7	35.67475766411853	37.0	35.0	37.0	33.0	37.0
8	35.67129009378202	37.0	35.0	37.0	33.0	37.0
9	37.25959492473796	39.0	37.0	39.0	34.0	39.0
10	37.210103239025926	39.0	37.0	39.0	34.0	39.0
11	37.33525100480731	39.0	37.0	39.0	34.0	39.0
12	37.28993616518244	39.0	37.0	39.0	34.0	39.0
13	37.370714792339825	39.0	37.0	39.0	34.0	39.0
14	38.435574119315945	40.0	38.0	41.0	34.0	41.0
15	38.44046024115376	40.0	38.0	41.0	34.0	41.0
16	38.25801875640318	40.0	38.0	41.0	34.0	41.0
17	38.36779888092048	40.0	38.0	41.0	34.0	41.0
18	38.485696272361885	40.0	38.0	41.0	34.0	41.0
19	38.50484671762944	40.0	38.0	41.0	34.0	41.0
20	38.56773583418709	40.0	38.0	41.0	34.0	41.0
21	38.55804239892821	40.0	38.0	41.0	34.0	41.0
22	38.507999054299	40.0	38.0	41.0	34.0	41.0
23	38.441327133737886	40.0	38.0	41.0	34.0	41.0
24	38.307510442115216	40.0	38.0	41.0	34.0	41.0
25	38.233351721963906	40.0	38.0	41.0	34.0	41.0
26	38.071636850815665	40.0	37.0	41.0	33.0	41.0
27	37.95815273071164	40.0	37.0	41.0	33.0	41.0
28	37.89282055323508	40.0	37.0	41.0	33.0	41.0
29	37.701000866892585	40.0	37.0	41.0	33.0	41.0
30	37.63133422649539	39.0	37.0	41.0	33.0	41.0
31	37.415320356214046	39.0	36.0	41.0	33.0	41.0
32	37.179446764914495	39.0	36.0	40.0	32.0	41.0
33	36.89203246906769	39.0	35.0	40.0	31.0	41.0
34	36.88076286547403	39.0	35.0	40.0	31.0	41.0
35	36.78272519505083	39.0	35.0	40.0	31.0	41.0
36	36.65734100401923	39.0	35.0	40.0	31.0	41.0
37	36.63251635274647	39.0	35.0	40.0	31.0	41.0
38	36.490345968949484	39.0	35.0	40.0	31.0	41.0
39	36.15146977697218	38.0	35.0	40.0	30.0	41.0
40	36.05358972338246	38.0	35.0	40.0	30.0	41.0
41	35.95965009062968	38.0	35.0	40.0	29.0	41.0
42	35.66467018677595	38.0	35.0	40.0	28.0	41.0
43	34.7723224840413	38.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	2.0
19	0.0
20	2.0
21	7.0
22	7.0
23	14.0
24	27.0
25	39.0
26	55.0
27	69.0
28	133.0
29	155.0
30	208.0
31	266.0
32	340.0
33	442.0
34	538.0
35	863.0
36	1338.0
37	2245.0
38	3415.0
39	2523.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.468121995429115	18.70911813381669	16.35274647332335	24.470013397430847
2	19.457798092836313	21.735361336590746	32.642446213255575	26.16439435731736
3	19.646938293009693	22.413113720545354	35.22736228229174	22.712585704153206
4	13.846638821026087	17.98408069981874	29.33249271022145	38.836787768933725
5	14.114587437938372	35.07762629048782	32.7921822050595	18.015604066514303
6	32.153834029474346	31.917408779257624	15.391283789108675	20.53747340215935
7	25.565450390101663	33.33596028055796	17.85798723303649	23.240602096303885
8	31.925289620931512	30.349121286153363	18.40176530853495	19.323823784380174
9	25.45511860666719	12.451729844747419	17.834344708014815	44.25880684057057
10	21.175821577744504	24.91922137284262	27.133737883205928	26.77121916620695
11	33.123177555362915	20.80542201907164	18.58302466703444	27.48837575853101
12	19.94641027661754	30.617069903065648	23.89471195523682	25.541807865079992
13	34.17921033966428	17.353613365907478	23.027819371108833	25.439356923319412
14	24.23358814721412	23.58735912995508	23.532193238237845	28.64685948459296
15	29.52951375206872	25.77035227362282	19.993695326660887	24.70643864764757
16	27.835132792182204	22.90960674600047	23.965639530301836	25.28962093151549
17	19.970052801639216	31.75979194577981	23.138151154543305	25.13200409803767
18	23.555835763259516	21.703837969895183	25.52604618173221	29.214280085113092
19	22.57861139569706	28.89116557648357	26.25108361573016	22.27913941208921
20	23.77649933012846	21.625029553156278	28.06367720072504	26.534793915990228
21	27.28347387500985	21.246749152809517	28.126723934116164	23.343053038064465
22	26.479628024272994	23.70557175506344	24.39120498069194	25.42359523997163
23	26.755457482859168	22.223973520371977	26.203798565686814	24.816770431082038
24	22.633777287414294	24.162660572149104	26.337772874142956	26.86578926629364
25	22.78351327921822	24.580345180865315	28.118843092442276	24.51729844747419
26	22.807155804239894	26.282606982425722	26.479628024272994	24.430609189061393
27	22.208211837024194	26.006777523839546	27.173142091575382	24.61186854756088
28	22.515564662305934	24.00504373867129	29.14335251004807	24.336039088974704
29	20.93151548585389	25.699424698557806	27.102214516510365	26.26684529907794
30	21.246749152809517	23.80014185515013	31.89376625423595	23.059342737804396
31	22.042714161872485	24.39120498069194	27.236188824966508	26.329892032469065
32	22.67318149578375	25.100480731342106	29.466467018677594	22.759870754196548
33	21.585625344786823	24.864055481125387	30.223027819371108	23.327291354716685
34	22.74410907084877	26.68452990779415	27.007644416423677	23.563716604933408
35	21.175821577744504	23.28788714634723	32.870990621798406	22.66530065410986
36	22.58649223737095	25.75459059027504	25.801875640318382	25.857041532035623
37	21.36496177791788	24.485775080778627	29.151233351721963	24.998029789581526
38	22.239735203719757	22.89384506265269	29.277326818504218	25.589092915123334
39	22.90960674600047	23.319410513042794	29.261565135156438	24.509417605800298
40	19.92276775159587	21.877216486720783	33.91914256442588	24.280873197257467
41	21.215225786113955	22.65741981243597	29.395539443612577	26.731814957837496
42	19.40263220111908	22.034833320198597	34.273780439750965	24.288754038931355
43	18.622428875403894	22.19245015367641	33.1310583970368	26.05406257388289
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	1.0
3	7.5
4	14.0
5	14.0
6	18.0
7	22.0
8	17.5
9	13.0
10	24.5
11	36.0
12	36.0
13	76.0
14	116.0
15	188.0
16	260.0
17	245.5
18	231.0
19	231.0
20	241.5
21	252.0
22	184.0
23	116.0
24	121.0
25	126.0
26	126.0
27	139.5
28	153.0
29	175.5
30	198.0
31	213.5
32	229.0
33	229.0
34	245.5
35	262.0
36	281.0
37	300.0
38	332.0
39	364.0
40	364.0
41	418.0
42	472.0
43	547.0
44	622.0
45	741.0
46	860.0
47	860.0
48	960.0
49	1060.0
50	1166.5
51	1273.0
52	1096.5
53	920.0
54	920.0
55	1101.5
56	1283.0
57	1175.5
58	1068.0
59	834.5
60	601.0
61	601.0
62	539.0
63	477.0
64	420.5
65	364.0
66	329.5
67	295.0
68	295.0
69	268.0
70	241.0
71	206.5
72	172.0
73	152.0
74	132.0
75	132.0
76	116.5
77	101.0
78	63.5
79	26.0
80	21.5
81	17.0
82	17.0
83	11.5
84	6.0
85	4.5
86	3.0
87	2.0
88	1.0
89	1.0
90	1.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	12689.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.04570888170856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.03928325292901	74.47395381826779
2	5.159003642807916	8.259122074237528
3	1.0239243871221817	2.4588226022539206
4	0.29536280397755243	0.9457010008668925
5	0.15752682878802796	0.6304673339112618
6	0.08860884119326573	0.4255654503901017
7	0.049227133996258735	0.275829458586177
8	0.019690853598503495	0.12609346678225233
9	0.009845426799251747	0.07092757506501694
>10	0.06891798759476223	1.2215304594530696
>50	0.019690853598503495	1.4658365513436835
>100	0.06891798759476223	9.646150208842304
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	345	2.718890377492316	Illumina PCR Primer Index 10 (95% over 21bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	176	1.3870281346047757	No Hit
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	160	1.2609346678225235	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	157	1.237292142800851	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	151	1.1900070927575066	No Hit
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	123	0.969343525888565	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	112	0.8826542674757665	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	95	0.7486799590196233	Illumina PCR Primer Index 10 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	91	0.7171565923240603	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	44	0.3467570336511939	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	40	0.3152336669556309	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22	0.17337851682559696	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14	0.11033178343447081	No Hit
ATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT	13	0.10245094176058002	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.00788084167389077	0.0
24	0.0	0.0	0.0	0.00788084167389077	0.0
25	0.0	0.0	0.0	0.01576168334778154	0.0
26	0.0	0.0	0.0	0.03152336669556308	0.0
27	0.0	0.0	0.0	0.04728505004334463	0.0
28	0.0	0.0	0.0	0.10245094176058003	0.0
29	0.0	0.0	0.0	0.23642525021672314	0.0
30	0.0	0.0	0.0	0.40980376704232013	0.0
31	0.0	0.0	0.0	0.8038458507368587	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTCTT	60	3.6585334E-8	27.750002	37
GCCGTCT	60	3.6585334E-8	27.750002	36
GGTATCA	100	0.0	25.900002	1
GTATCAA	125	1.2732926E-11	20.72	2
ATGCCGT	90	1.8315768E-6	18.5	34
TGCCGTC	95	3.0719002E-6	17.526316	35
TATGCCG	100	5.0112194E-6	16.650002	33
AATCTCG	120	1.5952701E-6	15.416667	26
TCTCGTA	110	1.2399909E-5	15.136364	28
CGTATGC	110	1.2399909E-5	15.136364	31
TCGTATG	110	1.2399909E-5	15.136364	30
TCTCCGA	110	1.2399909E-5	15.136364	1
GAGTACT	75	0.003813933	14.8	13
GTATGCC	115	1.8890607E-5	14.478261	32
ATCTCGT	115	1.8890607E-5	14.478261	27
TCCGAGC	115	1.8890607E-5	14.478261	3
CTCGTAT	115	1.8890607E-5	14.478261	29
CTCCGAG	115	1.8890607E-5	14.478261	2
ATCAACG	180	2.3064786E-9	14.388889	4
TATCAAC	185	3.3978722E-9	14.0	3
>>END_MODULE