FastQCFastQC Report
Fri 10 Feb 2017
ERR1632027.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632027.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51793
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT1430.2760990867491746TruSeq Adapter, Index 3 (95% over 21bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC620.11970729635278898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAATGA200.001825743237.01
CTGGGCA303.5526062E-430.8333329
CTCTTAG250.00544857429.61
CAGAGCT358.754983E-426.4285724
GTATCAA551.8575603E-523.5454541
GAGAGGG500.006946695518.57
TATCAAC752.019631E-417.2666662
CTCTTAT1452.763045E-916.58620837
CAACGCA803.3051748E-416.18755
ATCAACG803.3051748E-416.18753
TCAACGC803.3051748E-416.18754
AACGCAG855.242297E-415.2352946
TCTTATA800.006192103513.87537
ACGCAGA1000.001791742612.9500017
AGAGTAC1050.002585224812.33333311
TCTCTTA2001.897497E-712.02500136
ACCTGTC1100.003662996511.77272735
GCAGAGT1100.003662996511.7727279
CAGAGTA1100.003662996511.77272710
GTCTCTT3002.4556357E-1011.09999937
Produced by FastQC (version 0.11.5)