##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632027.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51793
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72448014210415	34.0	31.0	34.0	31.0	34.0
2	32.88720483463016	34.0	31.0	34.0	31.0	34.0
3	32.8672986697044	34.0	31.0	34.0	31.0	34.0
4	36.35578166933755	37.0	37.0	37.0	35.0	37.0
5	36.2584712219798	37.0	37.0	37.0	35.0	37.0
6	36.2970864788678	37.0	37.0	37.0	35.0	37.0
7	36.28544397891607	37.0	37.0	37.0	35.0	37.0
8	36.26524819956365	37.0	36.0	37.0	35.0	37.0
9	38.07304075840364	39.0	38.0	39.0	35.0	39.0
10	37.98210182843241	39.0	38.0	39.0	35.0	39.0
11	38.09449153360493	39.0	38.0	39.0	35.0	39.0
12	38.0476126117429	39.0	38.0	39.0	35.0	39.0
13	38.09192361902188	39.0	38.0	39.0	37.0	39.0
14	39.47099028826289	40.0	39.0	41.0	37.0	41.0
15	39.47954356766358	40.0	39.0	41.0	37.0	41.0
16	39.381846967736955	40.0	39.0	41.0	37.0	41.0
17	39.42335836889155	40.0	39.0	41.0	37.0	41.0
18	39.44212538373912	40.0	39.0	41.0	37.0	41.0
19	39.50010619195644	41.0	39.0	41.0	37.0	41.0
20	39.467032224431875	40.0	39.0	41.0	37.0	41.0
21	39.452590118355765	40.0	39.0	41.0	37.0	41.0
22	39.427007510667465	40.0	39.0	41.0	37.0	41.0
23	39.40047882918541	40.0	39.0	41.0	37.0	41.0
24	39.346591238198215	40.0	39.0	41.0	36.0	41.0
25	39.260942598420634	40.0	39.0	41.0	36.0	41.0
26	39.20381132585484	40.0	39.0	41.0	36.0	41.0
27	39.12194698125229	40.0	39.0	41.0	36.0	41.0
28	39.08603479234645	40.0	39.0	41.0	36.0	41.0
29	38.92738400942212	40.0	39.0	41.0	35.0	41.0
30	38.90892591662966	40.0	38.0	41.0	35.0	41.0
31	38.8404417585388	40.0	38.0	41.0	35.0	41.0
32	38.79188307300214	40.0	38.0	41.0	35.0	41.0
33	38.657115826463034	40.0	38.0	41.0	35.0	41.0
34	38.663776958276216	40.0	38.0	41.0	35.0	41.0
35	38.655474678045294	40.0	38.0	41.0	35.0	41.0
36	38.64717239781438	40.0	38.0	41.0	35.0	41.0
37	38.61985210356612	40.0	38.0	41.0	35.0	41.0
38	38.56667889483135	40.0	38.0	41.0	35.0	41.0
39	38.422624679010674	40.0	38.0	41.0	34.0	41.0
40	38.42443959608441	40.0	38.0	41.0	34.0	41.0
41	38.445349757689264	40.0	38.0	41.0	34.0	41.0
42	38.34054795049524	40.0	38.0	41.0	34.0	41.0
43	37.49143706678509	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	1.0
19	1.0
20	2.0
21	4.0
22	12.0
23	12.0
24	28.0
25	50.0
26	87.0
27	88.0
28	141.0
29	209.0
30	295.0
31	443.0
32	586.0
33	808.0
34	1280.0
35	1885.0
36	2999.0
37	5581.0
38	13392.0
39	23887.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.74902013785647	17.27839669453401	12.488174077578051	27.48440909003147
2	20.240186897843337	19.211090301778235	32.50053095978221	28.04819184059622
3	20.892784739250477	19.935126368428165	28.864904523777348	30.30718436854401
4	16.006023980074527	14.88811229316703	31.598864711447494	37.506999015310946
5	17.336319579866004	33.47749695904852	32.76697623230939	16.419207228776088
6	38.57664163110845	31.992740331705054	13.837777305813525	15.592840731372965
7	31.799664047265075	28.20458363099261	19.396443534840614	20.599308786901705
8	29.032880891240133	31.320834861853918	18.724538064989478	20.921746181916475
9	27.67555461162705	13.13884115614079	17.569941884038386	41.61566234819377
10	17.990848184117546	25.248585716216475	31.72822582202228	25.032340277643694
11	38.86818682061283	19.392582009151816	19.433128028884212	22.306103141351148
12	23.891259436603402	23.433668642480644	26.64066572702875	26.034406193887204
13	33.743942231575694	17.3884501766648	21.30403722510764	27.563570366651863
14	24.69445677987373	18.494777286505897	22.346649161083544	34.464116772536826
15	27.72961597127025	24.829610178981714	19.67640414727859	27.764369702469445
16	27.833877164867836	23.40470719981465	21.473944355414826	27.287471279902693
17	26.146390438862394	24.057305041221785	22.777209275384706	27.019095244531115
18	26.681211746761146	22.553240785434326	23.447183982391444	27.318363485413087
19	27.169694746394303	22.896916571737496	23.37188423145985	26.561504450408357
20	27.49020137856467	22.615025196455118	23.48966076496824	26.40511266001197
21	27.53653968683027	23.082269804799875	22.404572046415538	26.976618461954317
22	28.405382966810187	22.8216168208059	22.43739501477034	26.335605197613575
23	27.84160021624544	22.593786805166722	22.05124244589037	27.51337053269747
24	27.557778078118666	22.60923290792192	22.323480006950746	27.50950900700867
25	27.185140849149498	22.73859401849671	22.60537138223312	27.470893750120673
26	27.204448477593495	22.587994516633522	22.52234857992393	27.68520842584905
27	26.49392775085436	23.234800069507465	23.103508196088274	27.167763983549904
28	27.204448477593495	22.638194350587916	22.862162840538296	27.295194331280285
29	26.389666557256774	23.095785144710675	23.062962176355878	27.451586121676673
30	26.76809607475914	23.201977101152664	22.92973954009229	27.100187283995908
31	26.932210916533123	23.35836889154905	22.37754136659394	27.331878825323884
32	26.22169018979399	23.130538875909874	23.553375938833433	27.094394995462707
33	25.85484524935802	22.939393354314287	23.659567895275423	27.54619350105227
34	26.63680420133995	22.223080339041957	23.632537215453826	27.50757824416427
35	25.816229992470024	22.232734153263955	24.78713339640492	27.1639024578611
36	25.953314154422415	22.016488714691175	24.25424285135057	27.775954279535846
37	25.279477921726873	21.018284324136467	25.46290039194486	28.2393373621918
38	24.25810437703937	21.487459695325622	26.509373853609564	27.745062074025444
39	24.690595254184927	20.832931091074084	27.044195161508313	27.432278493232676
40	23.617091112698628	20.522078273125715	28.654451373737764	27.206379240437894
41	23.566891278744233	20.176471723978143	28.584943911339373	27.671693085938255
42	22.727009441430308	20.02007993358176	29.668101867047667	27.58480875794026
43	21.794450987585197	19.543181511015	30.235746143301217	28.426621358098586
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.5
4	3.0
5	3.0
6	3.0
7	3.0
8	4.5
9	6.0
10	6.0
11	6.0
12	6.0
13	19.0
14	32.0
15	30.0
16	28.0
17	36.5
18	45.0
19	45.0
20	60.0
21	75.0
22	88.5
23	102.0
24	143.0
25	184.0
26	184.0
27	233.5
28	283.0
29	350.5
30	418.0
31	516.5
32	615.0
33	615.0
34	771.5
35	928.0
36	1159.5
37	1391.0
38	1565.5
39	1740.0
40	1740.0
41	2056.0
42	2372.0
43	2633.0
44	2894.0
45	3074.0
46	3254.0
47	3254.0
48	3531.0
49	3808.0
50	3975.5
51	4143.0
52	4295.0
53	4447.0
54	4447.0
55	4538.0
56	4629.0
57	4529.5
58	4430.0
59	4226.0
60	4022.0
61	4022.0
62	3721.5
63	3421.0
64	3067.5
65	2714.0
66	2370.0
67	2026.0
68	2026.0
69	1718.5
70	1411.0
71	1153.0
72	895.0
73	755.0
74	615.0
75	615.0
76	485.5
77	356.0
78	279.0
79	202.0
80	172.0
81	142.0
82	142.0
83	108.0
84	74.0
85	61.0
86	48.0
87	31.5
88	15.0
89	15.0
90	13.5
91	12.0
92	7.0
93	2.0
94	1.5
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	51793.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.62075956210299
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.72067891686774	89.61442665997336
2	3.2811565509703233	6.143687370880235
3	0.583637526036833	1.6392176548954491
4	0.23716719255913715	0.8881509084239183
5	0.07218131947452	0.33788349776996895
6	0.03918414485759657	0.22010696426157972
7	0.018560910722019427	0.12163805919718881
8	0.012373940481346286	0.09267661653119147
9	0.006186970240673143	0.052130596798795205
>10	0.02474788096269257	0.4942752881663545
>50	0.002062323413557714	0.11970729635278898
>100	0.002062323413557714	0.2760990867491746
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT	143	0.2760990867491746	TruSeq Adapter, Index 3 (95% over 21bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC	62	0.11970729635278898	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.005792288533199467	0.0
27	0.0	0.0	0.0	0.03861525688799645	0.0
28	0.0	0.0	0.0	0.12356882204158863	0.0
29	0.0	0.0	0.0	0.28189137528237407	0.0
30	0.0	0.0	0.0	0.467244608344757	0.0
31	0.0	0.0	0.0	0.9402815052227135	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTAATGA	20	0.0018257432	37.0	1
CTGGGCA	30	3.5526062E-4	30.833332	9
CTCTTAG	25	0.005448574	29.6	1
CAGAGCT	35	8.754983E-4	26.428572	4
GTATCAA	55	1.8575603E-5	23.545454	1
GAGAGGG	50	0.0069466955	18.5	7
TATCAAC	75	2.019631E-4	17.266666	2
CTCTTAT	145	2.763045E-9	16.586208	37
CAACGCA	80	3.3051748E-4	16.1875	5
ATCAACG	80	3.3051748E-4	16.1875	3
TCAACGC	80	3.3051748E-4	16.1875	4
AACGCAG	85	5.242297E-4	15.235294	6
TCTTATA	80	0.0061921035	13.875	37
ACGCAGA	100	0.0017917426	12.950001	7
AGAGTAC	105	0.0025852248	12.333333	11
TCTCTTA	200	1.897497E-7	12.025001	36
ACCTGTC	110	0.0036629965	11.772727	35
GCAGAGT	110	0.0036629965	11.772727	9
CAGAGTA	110	0.0036629965	11.772727	10
GTCTCTT	300	2.4556357E-10	11.099999	37
>>END_MODULE