FastQCFastQC Report
Fri 10 Feb 2017
ERR1632026.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632026.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577520
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT41940.2658603377453218No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT36980.23441858106394847No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28530.18085349155636696No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17570.11137735179268726No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20000.019.33251
CTTATAC17800.017.77247237
TCTTATA31300.015.95846737
GTATAAC700.002593067715.8571421
TCTAGCG957.062339E-515.57894828
GTATAGA1557.214112E-915.51612951
GTGTACG750.00410530714.81
ACCGTCG800.006299993513.8758
GTATCAA27500.013.7236372
ATACCGT950.001245433213.6315796
TATACAC6500.013.37692437
CTCTTAT50050.013.34365737
TAACCCG1857.386916E-813.00000128
TAGCACA1901.0464282E-712.6578954
AGGACCG2401.1277734E-912.3333345
TTAGCAC1501.9445104E-512.3333333
GCGTTAT1653.805484E-612.3333331
ATCGTGC1050.002644321212.3333338
GAACCGC1653.805484E-612.3333336
TCTATCC1653.805484E-612.3333333
Produced by FastQC (version 0.11.5)