##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632026.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1577520
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.80235939956387	34.0	31.0	34.0	31.0	34.0
2	32.95729626248796	34.0	33.0	34.0	31.0	34.0
3	32.96016342106598	34.0	33.0	34.0	31.0	34.0
4	36.393476469395	37.0	37.0	37.0	35.0	37.0
5	36.312802373345505	37.0	37.0	37.0	35.0	37.0
6	36.34965515492672	37.0	37.0	37.0	35.0	37.0
7	36.338077488716465	37.0	37.0	37.0	35.0	37.0
8	36.32506846188955	37.0	37.0	37.0	35.0	37.0
9	38.10510674983519	39.0	39.0	39.0	37.0	39.0
10	38.04976291901212	39.0	38.0	39.0	37.0	39.0
11	38.153156219889446	39.0	39.0	39.0	37.0	39.0
12	38.125027257974544	39.0	38.0	39.0	37.0	39.0
13	38.1458054414524	39.0	39.0	39.0	37.0	39.0
14	39.55823317612455	41.0	39.0	41.0	37.0	41.0
15	39.583495613367816	41.0	39.0	41.0	37.0	41.0
16	39.469632080734314	41.0	39.0	41.0	37.0	41.0
17	39.52061717125615	41.0	39.0	41.0	37.0	41.0
18	39.538476215832446	41.0	39.0	41.0	37.0	41.0
19	39.59117475531214	41.0	39.0	41.0	37.0	41.0
20	39.56980196764542	41.0	39.0	41.0	37.0	41.0
21	39.54790937674324	41.0	39.0	41.0	37.0	41.0
22	39.523550890004564	41.0	39.0	41.0	37.0	41.0
23	39.48097393376946	41.0	39.0	41.0	37.0	41.0
24	39.42093158882296	41.0	39.0	41.0	37.0	41.0
25	39.36016976012982	41.0	39.0	41.0	36.0	41.0
26	39.27217214361783	40.0	39.0	41.0	36.0	41.0
27	39.20117653025001	40.0	39.0	41.0	36.0	41.0
28	39.1357054110249	40.0	39.0	41.0	36.0	41.0
29	38.988686672752166	40.0	39.0	41.0	35.0	41.0
30	38.94204637659111	40.0	39.0	41.0	35.0	41.0
31	38.86537920279933	40.0	38.0	41.0	35.0	41.0
32	38.799630432577715	40.0	38.0	41.0	35.0	41.0
33	38.661193518941126	40.0	38.0	41.0	35.0	41.0
34	38.654345428267156	40.0	38.0	41.0	35.0	41.0
35	38.62199908717481	40.0	38.0	41.0	35.0	41.0
36	38.592807698159135	40.0	38.0	41.0	35.0	41.0
37	38.545305289314875	40.0	38.0	41.0	35.0	41.0
38	38.473950884933316	40.0	38.0	41.0	35.0	41.0
39	38.28260053755261	40.0	38.0	41.0	34.0	41.0
40	38.26857789441655	40.0	38.0	41.0	34.0	41.0
41	38.25543828287439	40.0	38.0	41.0	34.0	41.0
42	38.11010827121051	40.0	38.0	41.0	34.0	41.0
43	37.278773010801764	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	4.0
12	1.0
13	0.0
14	2.0
15	7.0
16	2.0
17	11.0
18	14.0
19	36.0
20	71.0
21	159.0
22	291.0
23	544.0
24	905.0
25	1362.0
26	2218.0
27	3411.0
28	4981.0
29	7035.0
30	9942.0
31	13405.0
32	17969.0
33	25209.0
34	37156.0
35	55534.0
36	88721.0
37	166716.0
38	374750.0
39	767062.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07939043562047	17.968456818297074	12.5285891779502	26.42356356813226
2	19.57914955119428	19.92957300065926	33.62797302094427	26.863304427202188
3	20.704143212130433	21.362011258177393	28.930029413256253	29.003816116435928
4	15.648359450276384	15.451277955271566	32.483645215274606	36.41671737917744
5	16.662736447081492	34.1457477559714	32.59730463005223	16.594211166894873
6	35.90908514630559	32.78215173183224	15.372863735483545	15.935899386378619
7	30.24367361428064	28.91266037831533	20.49660226177798	20.347063745626045
8	27.80940970637456	32.046693544297376	19.493889142451444	20.650007606876617
9	26.725239616613415	14.11982098483696	19.448691617221968	39.706247781327654
10	18.240529438612505	25.84353922612709	31.83953293777575	24.07639839748466
11	36.938549115066685	20.810956437953244	20.538630255083927	21.71186419189614
12	22.551473198438053	24.566344642223235	28.208390384907954	24.673791774430754
13	31.678013590952887	18.892121811450885	23.245283736497793	26.184580861098432
14	23.622521426035803	19.98256757442061	24.178710887976063	32.216200111567524
15	26.295260915867946	26.324927734672144	21.42888838176378	25.950922967696133
16	25.895012424565138	24.962409351386988	23.40337998884325	25.739198235204626
17	24.536804604695977	25.477521679598357	24.611351995537298	25.374321720168364
18	24.82383741569045	24.287425832952987	25.555618946194024	25.333117805162537
19	25.488234697499873	24.5975962269892	25.3473806988184	24.566788376692532
20	25.717265074293827	24.214399817434963	25.189474618388356	24.878860489882854
21	26.16461280998022	24.136492722754703	24.71600993965211	24.98288452761296
22	26.29824027587606	24.12191287590649	24.394872965160506	25.184973883056948
23	25.686076880166336	24.223464678736246	24.63132004665551	25.45913839444191
24	25.478535929813884	24.54219280896597	24.504348597799076	25.47492266342107
25	25.645380090268272	24.077285866423246	24.631827171763273	25.64550687154521
26	25.362531061412852	24.37173538211877	24.863139611542167	25.40259394492621
27	25.225543891678075	24.30473147725544	24.77122318575993	25.698501445306558
28	25.15010903189817	24.34701303311527	25.094642223236473	25.408235711750088
29	24.99866879659212	24.576043409909225	25.356445560119685	25.068842233378973
30	24.96621278969522	24.689132308940614	25.056607840154165	25.28804706121
31	25.301866220396573	24.324509356458236	24.908717480602466	25.464906942542726
32	24.66719914802982	24.51854810081647	25.09197981642071	25.722272934733
33	24.616042902784116	24.123244079314368	25.567916730057306	25.69279628784421
34	25.0829783457579	23.528005984076273	25.391563973832344	25.997451696333485
35	24.588531365687917	23.54740351944825	26.138495866930374	25.725569247933468
36	24.495790861605556	23.1816395354734	26.11713322176581	26.205436381155227
37	24.209708910188144	22.544310056290886	26.961559916831483	26.284421116689487
38	23.72381966631168	22.365928799634872	27.79666818804199	26.11358334601146
39	23.35894315127542	22.110971651706475	28.53643693899285	25.993648258025253
40	23.006110857548556	22.087643896749327	29.0556696587048	25.85057558699731
41	22.287387798569906	22.171002586338048	29.500418378213904	26.041191236878134
42	21.986345656473453	22.58811298747401	29.523619351894116	25.90192200415843
43	21.421725239616613	22.41968406105786	29.736168162685733	26.42242253663979
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	156.0
1	170.5
2	185.0
3	475.5
4	766.0
5	766.0
6	996.5
7	1227.0
8	1226.0
9	1225.0
10	1859.5
11	2494.0
12	2494.0
13	4102.0
14	5710.0
15	7808.0
16	9906.0
17	9350.0
18	8794.0
19	8794.0
20	9981.5
21	11169.0
22	10330.0
23	9491.0
24	11363.0
25	13235.0
26	13235.0
27	15401.5
28	17568.0
29	21325.5
30	25083.0
31	29304.0
32	33525.0
33	33525.0
34	39097.5
35	44670.0
36	50534.5
37	56399.0
38	62811.0
39	69223.0
40	69223.0
41	75646.5
42	82070.0
43	87945.5
44	93821.0
45	99165.5
46	104510.0
47	104510.0
48	108634.0
49	112758.0
50	114821.0
51	116884.0
52	118061.0
53	119238.0
54	119238.0
55	117634.5
56	116031.0
57	112112.0
58	108193.0
59	103975.5
60	99758.0
61	99758.0
62	92480.0
63	85202.0
64	76667.5
65	68133.0
66	60202.5
67	52272.0
68	52272.0
69	44996.0
70	37720.0
71	32139.0
72	26558.0
73	21844.5
74	17131.0
75	17131.0
76	13900.5
77	10670.0
78	8722.0
79	6774.0
80	5560.5
81	4347.0
82	4347.0
83	3317.0
84	2287.0
85	1760.5
86	1234.0
87	964.5
88	695.0
89	695.0
90	510.0
91	325.0
92	188.5
93	52.0
94	35.0
95	18.0
96	18.0
97	13.5
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1577520.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.3851546431404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.48990783271071	60.87605537876304
2	8.146885616725472	11.468396079863972
3	2.1965183586814505	4.6380685305687255
4	0.9487581263978448	2.6711394978179386
5	0.5413901804167821	1.9052915785456443
6	0.3407629676750683	1.4390792505879082
7	0.23243460020521736	1.1451961695902517
8	0.16547533338912382	0.9317605544174955
9	0.13767514729022204	0.8721257879285469
>10	0.7413050099465337	9.5851576859204
>50	0.04526315415532336	2.1829563791556046
>100	0.013172612955844428	1.4493591510745656
>500	9.021189009680393E-5	0.041589734819993625
>1k	3.608475603872157E-4	0.7938242209458937
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4194	0.2658603377453218	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3698	0.23441858106394847	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2853	0.18085349155636696	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1757	0.11137735179268726	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	6.339063847051067E-5	6.339063847051067E-5
2	0.0	0.0	0.0	6.339063847051067E-5	6.339063847051067E-5
3	0.0	0.0	0.0	6.339063847051067E-5	6.339063847051067E-5
4	0.0	0.0	0.0	6.339063847051067E-5	6.339063847051067E-5
5	0.0	0.0	0.0	6.339063847051067E-5	6.339063847051067E-5
6	0.0	0.0	0.0	6.339063847051067E-5	6.339063847051067E-5
7	0.0	0.0	0.0	6.339063847051067E-5	6.339063847051067E-5
8	0.0	0.0	0.0	6.339063847051067E-5	6.339063847051067E-5
9	0.0	0.0	0.0	1.2678127694102135E-4	6.339063847051067E-5
10	6.339063847051067E-5	0.0	0.0	1.2678127694102135E-4	6.339063847051067E-5
11	6.339063847051067E-5	6.339063847051067E-5	0.0	1.2678127694102135E-4	6.339063847051067E-5
12	6.339063847051067E-5	6.339063847051067E-5	0.0	1.2678127694102135E-4	6.339063847051067E-5
13	6.339063847051067E-5	6.339063847051067E-5	0.0	2.535625538820427E-4	6.339063847051067E-5
14	6.339063847051067E-5	6.339063847051067E-5	0.0	3.169531923525534E-4	6.339063847051067E-5
15	6.339063847051067E-5	6.339063847051067E-5	0.0	3.8034383082306405E-4	6.339063847051067E-5
16	6.339063847051067E-5	1.2678127694102135E-4	0.0	0.0013312034078807242	6.339063847051067E-5
17	6.339063847051067E-5	1.2678127694102135E-4	0.0	0.0023454536234088948	6.339063847051067E-5
18	6.339063847051067E-5	1.2678127694102135E-4	0.0	0.0026624068157614484	6.339063847051067E-5
19	6.339063847051067E-5	1.2678127694102135E-4	0.0	0.003296313200466555	6.339063847051067E-5
20	6.339063847051067E-5	1.2678127694102135E-4	0.0	0.0036132663928191084	6.339063847051067E-5
21	6.339063847051067E-5	1.2678127694102135E-4	0.0	0.004120391500583194	6.339063847051067E-5
22	6.339063847051067E-5	1.2678127694102135E-4	0.0	0.005895329377757493	6.339063847051067E-5
23	6.339063847051067E-5	1.9017191541153202E-4	0.0	0.00855773619351894	6.339063847051067E-5
24	6.339063847051067E-5	2.535625538820427E-4	0.0	0.012361174501749582	6.339063847051067E-5
25	6.339063847051067E-5	2.535625538820427E-4	0.0	0.016037831533039202	6.339063847051067E-5
26	6.339063847051067E-5	2.535625538820427E-4	0.0	0.023074192403265886	6.339063847051067E-5
27	6.339063847051067E-5	2.535625538820427E-4	0.0	0.058509559308281354	6.339063847051067E-5
28	6.339063847051067E-5	2.535625538820427E-4	0.0	0.16418175363862264	6.339063847051067E-5
29	6.339063847051067E-5	2.535625538820427E-4	0.0	0.3010421420964552	6.339063847051067E-5
30	6.339063847051067E-5	2.535625538820427E-4	0.0	0.5036386226482074	6.339063847051067E-5
31	6.339063847051067E-5	2.535625538820427E-4	0.0	1.050256098179421	6.339063847051067E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2000	0.0	19.3325	1
CTTATAC	1780	0.0	17.772472	37
TCTTATA	3130	0.0	15.958467	37
GTATAAC	70	0.0025930677	15.857142	1
TCTAGCG	95	7.062339E-5	15.578948	28
GTATAGA	155	7.214112E-9	15.5161295	1
GTGTACG	75	0.004105307	14.8	1
ACCGTCG	80	0.0062999935	13.875	8
GTATCAA	2750	0.0	13.723637	2
ATACCGT	95	0.0012454332	13.631579	6
TATACAC	650	0.0	13.376924	37
CTCTTAT	5005	0.0	13.343657	37
TAACCCG	185	7.386916E-8	13.000001	28
TAGCACA	190	1.0464282E-7	12.657895	4
AGGACCG	240	1.1277734E-9	12.333334	5
TTAGCAC	150	1.9445104E-5	12.333333	3
GCGTTAT	165	3.805484E-6	12.333333	1
ATCGTGC	105	0.0026443212	12.333333	8
GAACCGC	165	3.805484E-6	12.333333	6
TCTATCC	165	3.805484E-6	12.333333	3
>>END_MODULE