FastQCFastQC Report
Fri 10 Feb 2017
ERR1632025.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632025.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995538
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9100.022.1593421
TAACGTG656.905388E-519.9230775
CTTATAC16450.017.76899537
AAGCGCG904.4485976E-516.4444457
CGCACTA803.384126E-416.187529
TCTTATA27700.015.02707637
ATCGTAC1152.2113465E-514.4782625
CGTATAT908.27778E-414.38888810
GCGTATA908.27778E-414.3888889
AGCGTAT1051.6565948E-414.0952398
TATACAC6450.013.76744237
GTATCAA14550.013.6048112
TCGTTTA2457.2759576E-1213.59183830
CTCTTAT42900.013.32517437
ACGGACC2102.042725E-913.2142878
GCCGTAA850.00940790213.05882429
CGAACGA2152.903107E-912.90697716
GACCGTT1307.002854E-512.8076937
TATTAGC3050.012.7377052
CGTTTAT2704.3655746E-1112.33333431
Produced by FastQC (version 0.11.5)