FastQCFastQC Report
Fri 10 Feb 2017
ERR1632024.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632024.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059065
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT26490.25012629064316166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25700.24266688069193107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16640.15711972352971726No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC13880.1310590001557978No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11460.10820865574823076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA603.72593E-521.58333426
GGTATCA15600.020.2788471
TAACGCA656.9014735E-519.9230774
GATATAT801.6164748E-518.51
CGAGCTA609.236161E-418.522
GTATAAC852.7227874E-517.4117641
TTAGTAC752.0670732E-417.2666663
ATACCGC1251.6572267E-716.2827
TATCCGG803.382251E-416.187510
AGCCGTA700.002592313815.85714234
CGACGTT700.002592313815.85714224
CTATCCG700.002592313815.8571429
TAGACAC1059.341635E-615.8571425
TAAGATA957.058837E-515.5789484
CTTATAC8200.015.34146437
CGTTATT855.363655E-415.23529428
GCGAAAG1101.451575E-515.13636418
TCTAGCG1101.451575E-515.13636428
TCTTATA13550.015.01845137
GTATCAA21150.014.9574472
Produced by FastQC (version 0.11.5)