##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632024.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1059065
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.69385826176863	34.0	31.0	34.0	31.0	34.0
2	32.85526100853111	34.0	31.0	34.0	31.0	34.0
3	32.84776288518646	34.0	31.0	34.0	31.0	34.0
4	36.31962816257737	37.0	37.0	37.0	35.0	37.0
5	36.23069122291833	37.0	37.0	37.0	35.0	37.0
6	36.26729804119672	37.0	37.0	37.0	35.0	37.0
7	36.25571518273194	37.0	37.0	37.0	35.0	37.0
8	36.23946405555844	37.0	37.0	37.0	35.0	37.0
9	38.00969156756195	39.0	38.0	39.0	35.0	39.0
10	37.94667560536889	39.0	38.0	39.0	35.0	39.0
11	38.05298919329786	39.0	38.0	39.0	35.0	39.0
12	38.021496319867055	39.0	38.0	39.0	35.0	39.0
13	38.04191433009306	39.0	38.0	39.0	35.0	39.0
14	39.42385689263643	40.0	39.0	41.0	37.0	41.0
15	39.45068716273317	40.0	39.0	41.0	37.0	41.0
16	39.31942137640277	40.0	39.0	41.0	37.0	41.0
17	39.37444538342783	40.0	39.0	41.0	37.0	41.0
18	39.38011359076166	40.0	39.0	41.0	37.0	41.0
19	39.42965351512891	40.0	39.0	41.0	37.0	41.0
20	39.40448225557449	40.0	39.0	41.0	37.0	41.0
21	39.386532460236154	40.0	39.0	41.0	37.0	41.0
22	39.36075783828188	40.0	39.0	41.0	36.0	41.0
23	39.31541029115304	40.0	39.0	41.0	36.0	41.0
24	39.25724766657382	40.0	39.0	41.0	36.0	41.0
25	39.19407685080708	40.0	39.0	41.0	36.0	41.0
26	39.08916733156133	40.0	39.0	41.0	36.0	41.0
27	39.01650512480348	40.0	39.0	41.0	35.0	41.0
28	38.945568024625494	40.0	39.0	41.0	35.0	41.0
29	38.80420087530038	40.0	38.0	41.0	35.0	41.0
30	38.752231449438895	40.0	38.0	41.0	35.0	41.0
31	38.669965488426115	40.0	38.0	41.0	35.0	41.0
32	38.598028449623015	40.0	38.0	41.0	35.0	41.0
33	38.44183312638979	40.0	38.0	41.0	34.0	41.0
34	38.43220198949073	40.0	38.0	41.0	34.0	41.0
35	38.389936406169596	40.0	38.0	41.0	34.0	41.0
36	38.34908716651008	40.0	38.0	41.0	34.0	41.0
37	38.2996407208245	40.0	38.0	41.0	34.0	41.0
38	38.21894501281791	40.0	38.0	41.0	34.0	41.0
39	38.01521058669675	40.0	38.0	41.0	33.0	41.0
40	37.99468493435247	40.0	37.0	41.0	33.0	41.0
41	37.96422504756554	40.0	37.0	41.0	33.0	41.0
42	37.80864441748146	40.0	37.0	41.0	33.0	41.0
43	36.932943681454866	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	0.0
14	1.0
15	2.0
16	2.0
17	7.0
18	16.0
19	33.0
20	71.0
21	126.0
22	277.0
23	483.0
24	725.0
25	1179.0
26	1875.0
27	2744.0
28	3985.0
29	5583.0
30	7730.0
31	10444.0
32	14229.0
33	19495.0
34	28502.0
35	43087.0
36	65711.0
37	119546.0
38	267821.0
39	465387.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.415182259823524	17.345488709380444	12.177439533928512	27.06188949686752
2	20.453796509184986	19.27785357839226	33.01327114010944	27.25507877231331
3	21.509633497471828	20.735743320759347	28.18287829358916	29.57174488817967
4	16.057654629319256	15.048934673509178	31.85772355804413	37.03568713912744
5	17.051455765226876	33.905945338576956	32.027873643260804	17.014725252935374
6	36.80708927214099	31.907862123665687	14.92599604368004	16.35905256051328
7	31.082889152223895	28.147752970780832	19.923045327718317	20.84631254927696
8	28.50674887754765	31.365024809619808	18.949828386359666	21.17839792647288
9	27.42277386184984	13.603225486632075	18.797335385457927	40.176665266060155
10	18.977305453395214	25.1602120738576	30.985255862482475	24.877226610264714
11	37.45558582334418	20.429529821115796	19.652995802901614	22.461888552638413
12	23.36088908612786	24.131191192230883	27.077280431323857	25.4306392903174
13	31.994259087024872	18.134486551816934	22.864979958737187	27.006274402421006
14	24.14120002077304	19.706628016221856	23.55719431762923	32.59497764537587
15	27.552416518344007	25.280601285095816	20.70203434161265	26.464947854947525
16	26.378362045766785	24.156496532318602	22.638837087430893	26.82630433448372
17	25.367848054651983	24.98722930131767	23.897588910973358	25.747333733056987
18	24.98222488704659	23.255135426059777	25.004414271078733	26.758225415814895
19	25.997271177878602	23.855570715678454	24.831337075628028	25.315821030814917
20	25.92352688456327	23.52924513603981	24.822839013658275	25.724388965738648
21	27.311165981313707	23.067705948171263	23.96670648166071	25.65442158885432
22	27.359982626184415	23.325669340408755	23.69127485092983	25.623073182476997
23	26.423779465849595	23.492986738302182	23.6841931326217	26.399040663226526
24	26.236727679604176	23.912413307965043	23.72866632359676	26.122192688834016
25	26.340309612724432	23.278552307931996	24.091439146794578	26.289698932548994
26	26.409143914679454	23.538687427117317	24.228635636150756	25.82353302205247
27	26.28261721424086	23.502712298112012	23.87974298083687	26.334927506810253
28	25.626566830175673	23.41697629512825	24.779121205969417	26.17733566872666
29	25.6628252279133	23.424718973811807	25.067677621298028	25.844778176976863
30	25.329512352877302	23.939795952089817	24.73672531903141	25.99396637600147
31	25.9680944984491	23.587692917809576	24.32523027387365	26.11898230986767
32	25.292687417675026	23.694390806985407	24.69801192561363	26.314909849725936
33	25.45632232204822	23.63613187103719	24.73039898400948	26.17714682290511
34	26.02559805111112	23.291393823797407	24.508693989509613	26.174314135581856
35	25.70824264799611	23.37448598527947	24.97287701887986	25.94439434784456
36	25.480966701760515	23.33615028350479	24.531638756827956	26.651244257906736
37	24.986001803477595	22.556311463413483	25.668301756738256	26.789384976370666
38	24.91121885814374	22.33819453952307	26.434260408945626	26.316326193387564
39	24.163861519359056	22.251986421985432	27.156029138910263	26.428122919745245
40	24.125903509227477	21.676950895365252	28.06343331145869	26.13371228394858
41	23.218027222125176	22.085329984467432	28.2444420314145	26.452200761992888
42	23.22501451752253	22.495975223428214	27.95201427674411	26.326995982305146
43	22.7115427287277	21.766463814780018	28.531487680170713	26.990505776321566
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	70.0
2	69.0
3	225.5
4	382.0
5	382.0
6	477.5
7	573.0
8	616.5
9	660.0
10	938.5
11	1217.0
12	1217.0
13	2257.0
14	3297.0
15	4632.5
16	5968.0
17	5343.5
18	4719.0
19	4719.0
20	5539.5
21	6360.0
22	5482.5
23	4605.0
24	5460.5
25	6316.0
26	6316.0
27	7743.5
28	9171.0
29	11353.5
30	13536.0
31	16315.0
32	19094.0
33	19094.0
34	23086.0
35	27078.0
36	30906.0
37	34734.0
38	38361.0
39	41988.0
40	41988.0
41	46552.0
42	51116.0
43	54792.0
44	58468.0
45	62540.0
46	66612.0
47	66612.0
48	69948.0
49	73284.0
50	75446.0
51	77608.0
52	80993.0
53	84378.0
54	84378.0
55	82798.5
56	81219.0
57	78349.5
58	75480.0
59	71593.0
60	67706.0
61	67706.0
62	65666.0
63	63626.0
64	55579.0
65	47532.0
66	42416.5
67	37301.0
68	37301.0
69	32364.0
70	27427.0
71	24480.0
72	21533.0
73	20223.0
74	18913.0
75	18913.0
76	16657.0
77	14401.0
78	10335.5
79	6270.0
80	4665.0
81	3060.0
82	3060.0
83	2314.5
84	1569.0
85	1258.5
86	948.0
87	707.5
88	467.0
89	467.0
90	349.5
91	232.0
92	148.0
93	64.0
94	36.5
95	9.0
96	9.0
97	6.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1059065.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.18706213286875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.89101439226555	57.29357016109474
2	7.108398899114768	9.267512814036204
3	1.8402256658665461	3.5987671445802687
4	0.83424495196599	2.1752791007135635
5	0.47054422202078017	1.533669771856549
6	0.2999436394098598	1.1731466795141583
7	0.2194725912071679	1.0014741407638348
8	0.16916009988844383	0.8821639953464216
9	0.12627895977809078	0.7408578957415592
>10	0.8992901481972946	11.55523824041507
>50	0.09178239027512684	4.113178835833693
>100	0.04732151271019064	5.186448735159147
>500	0.0015967375188591145	0.5876163263267077
>1k	7.257897812995975E-4	0.891076158618133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2649	0.25012629064316166	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2570	0.24266688069193107	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1664	0.15711972352971726	No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	1388	0.1310590001557978	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1146	0.10820865574823076	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	9.442291077507046E-5	0.0	0.0	0.0	0.0
11	9.442291077507046E-5	0.0	0.0	0.0	0.0
12	9.442291077507046E-5	0.0	0.0	0.0	0.0
13	9.442291077507046E-5	0.0	0.0	0.0	0.0
14	9.442291077507046E-5	9.442291077507046E-5	0.0	1.8884582155014092E-4	9.442291077507046E-5
15	9.442291077507046E-5	9.442291077507046E-5	0.0	2.8326873232521136E-4	9.442291077507046E-5
16	9.442291077507046E-5	9.442291077507046E-5	0.0	0.001416343661626057	9.442291077507046E-5
17	1.8884582155014092E-4	9.442291077507046E-5	0.0	0.0031159560555773253	9.442291077507046E-5
18	1.8884582155014092E-4	9.442291077507046E-5	0.0	0.0035880706094526776	1.8884582155014092E-4
19	1.8884582155014092E-4	9.442291077507046E-5	0.0	0.005098837181853805	1.8884582155014092E-4
20	1.8884582155014092E-4	9.442291077507046E-5	0.0	0.006231912111154651	1.8884582155014092E-4
21	1.8884582155014092E-4	9.442291077507046E-5	0.0	0.00783710159433085	1.8884582155014092E-4
22	1.8884582155014092E-4	9.442291077507046E-5	0.0	0.011991709668433949	1.8884582155014092E-4
23	1.8884582155014092E-4	9.442291077507046E-5	0.0	0.014635551170135921	1.8884582155014092E-4
24	2.8326873232521136E-4	9.442291077507046E-5	0.0	0.020678617459740433	1.8884582155014092E-4
25	2.8326873232521136E-4	9.442291077507046E-5	0.0	0.02445553389074325	1.8884582155014092E-4
26	2.8326873232521136E-4	9.442291077507046E-5	0.0	0.03153725219887354	1.8884582155014092E-4
27	2.8326873232521136E-4	9.442291077507046E-5	0.0	0.05778682139434312	1.8884582155014092E-4
28	2.8326873232521136E-4	9.442291077507046E-5	0.0	0.12832073574332076	1.8884582155014092E-4
29	2.8326873232521136E-4	9.442291077507046E-5	0.0	0.22000538210591417	1.8884582155014092E-4
30	2.8326873232521136E-4	9.442291077507046E-5	0.0	0.3481372720276848	1.8884582155014092E-4
31	2.8326873232521136E-4	9.442291077507046E-5	0.0	0.6967466586092449	1.8884582155014092E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTTA	60	3.72593E-5	21.583334	26
GGTATCA	1560	0.0	20.278847	1
TAACGCA	65	6.9014735E-5	19.923077	4
GATATAT	80	1.6164748E-5	18.5	1
CGAGCTA	60	9.236161E-4	18.5	22
GTATAAC	85	2.7227874E-5	17.411764	1
TTAGTAC	75	2.0670732E-4	17.266666	3
ATACCGC	125	1.6572267E-7	16.28	27
TATCCGG	80	3.382251E-4	16.1875	10
AGCCGTA	70	0.0025923138	15.857142	34
CGACGTT	70	0.0025923138	15.857142	24
CTATCCG	70	0.0025923138	15.857142	9
TAGACAC	105	9.341635E-6	15.857142	5
TAAGATA	95	7.058837E-5	15.578948	4
CTTATAC	820	0.0	15.341464	37
CGTTATT	85	5.363655E-4	15.235294	28
GCGAAAG	110	1.451575E-5	15.136364	18
TCTAGCG	110	1.451575E-5	15.136364	28
TCTTATA	1355	0.0	15.018451	37
GTATCAA	2115	0.0	14.957447	2
>>END_MODULE