FastQCFastQC Report
Fri 10 Feb 2017
ERR1632023.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632023.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483453
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.1276750931778762No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA16770.11304705979899599No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC15480.10435113212215015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8950.024.1843571
CCGTATA555.144062E-420.1818182
TACTATA1101.7553248E-920.1818182
TGCGTTA656.903858E-519.92307737
TTAGAGT1800.018.54
ATGCGTT701.2195003E-418.536
CTTATAC12750.017.70196237
TAGAGTG2100.017.619055
GGTTAGA953.6078163E-617.5263161
CGGCCTT4900.016.98979624
TCTTATA23000.016.6537
GACGGAT803.383394E-416.18757
GCGCAAG3700.016.01
CGCAAGA3700.016.02
ATTAGAG2100.015.8571443
CCCGTAT700.002592969515.8571431
GTCTTAT700.002592969515.8571431
GTATCAA13800.015.8188422
AGACGGA3750.015.7866666
ACGGACC3550.015.6338028
Produced by FastQC (version 0.11.5)