##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632023.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1483453
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10308381863126	33.0	31.0	34.0	30.0	34.0
2	32.274818952808076	34.0	31.0	34.0	30.0	34.0
3	32.29362979480981	34.0	31.0	34.0	30.0	34.0
4	35.96991613485564	37.0	35.0	37.0	35.0	37.0
5	35.69246818065689	37.0	35.0	37.0	33.0	37.0
6	35.68871747200619	37.0	35.0	37.0	33.0	37.0
7	35.680736767528195	37.0	35.0	37.0	33.0	37.0
8	35.64370222716864	37.0	35.0	37.0	33.0	37.0
9	37.338518308298276	39.0	37.0	39.0	34.0	39.0
10	37.18399841450993	39.0	37.0	39.0	34.0	39.0
11	37.31549364893933	39.0	37.0	39.0	34.0	39.0
12	37.25746619542379	39.0	37.0	39.0	34.0	39.0
13	37.30077326346032	39.0	37.0	39.0	34.0	39.0
14	38.51166636219685	40.0	38.0	41.0	35.0	41.0
15	38.50314570127938	40.0	38.0	41.0	34.0	41.0
16	38.38229050735008	40.0	38.0	41.0	34.0	41.0
17	38.42642537377322	40.0	38.0	41.0	34.0	41.0
18	38.425018520977744	40.0	38.0	41.0	34.0	41.0
19	38.48571811847089	40.0	38.0	41.0	34.0	41.0
20	38.45215318584411	40.0	38.0	41.0	34.0	41.0
21	38.41480653583228	40.0	38.0	41.0	34.0	41.0
22	38.39156751174456	40.0	38.0	41.0	34.0	41.0
23	38.33637735742218	40.0	38.0	41.0	34.0	41.0
24	38.26140430468643	40.0	38.0	41.0	34.0	41.0
25	38.17552224438523	40.0	38.0	41.0	34.0	41.0
26	38.065706833988	40.0	38.0	41.0	33.0	41.0
27	37.97595946753958	40.0	37.0	41.0	33.0	41.0
28	37.87824959739203	40.0	37.0	41.0	33.0	41.0
29	37.729284985773056	40.0	37.0	41.0	33.0	41.0
30	37.66421046032466	40.0	37.0	41.0	33.0	41.0
31	37.571531420274184	39.0	37.0	41.0	33.0	41.0
32	37.4774933887356	39.0	37.0	40.0	32.0	41.0
33	37.31699892076122	39.0	36.0	40.0	32.0	41.0
34	37.31247097144298	39.0	36.0	40.0	32.0	41.0
35	37.272328142516145	39.0	36.0	40.0	32.0	41.0
36	37.24643045650924	39.0	36.0	40.0	32.0	41.0
37	37.21825430263042	39.0	36.0	40.0	32.0	41.0
38	37.13495203420668	39.0	36.0	40.0	31.0	41.0
39	36.950268731129334	39.0	36.0	40.0	31.0	41.0
40	36.95513238370208	39.0	36.0	40.0	31.0	41.0
41	36.958419983646266	39.0	36.0	40.0	31.0	41.0
42	36.82364995722817	39.0	35.0	40.0	31.0	41.0
43	35.88004810398442	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	3.0
15	10.0
16	13.0
17	35.0
18	55.0
19	139.0
20	262.0
21	481.0
22	833.0
23	1416.0
24	2241.0
25	3517.0
26	4908.0
27	7256.0
28	10067.0
29	14232.0
30	19369.0
31	25739.0
32	34492.0
33	46959.0
34	65999.0
35	96724.0
36	151221.0
37	256726.0
38	445944.0
39	294810.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.69943166382757	17.91374583488658	12.489644093880965	26.897178407404887
2	19.504898368873164	20.500548382725977	33.15035933056188	26.844193917838986
3	20.34449355658723	21.403104783232095	29.783147831444612	28.469253828736065
4	15.556542741832738	15.432979676471046	33.03353729440703	35.97694028728918
5	16.093533128450986	35.08038340277717	33.04688453223662	15.779198936535233
6	36.017656103698606	33.703797828444856	15.273217284268528	15.00532878358802
7	30.69028813181139	29.5551662236687	20.11610748705891	19.638438157461007
8	28.375283881592473	32.25562252393571	19.524716994741322	19.84437659973049
9	27.364601372608366	13.870072054861193	18.154737629031725	40.61058894349871
10	17.616938318908655	26.069447431094883	31.590552582387172	24.723061667609286
11	37.88781983655701	20.43671083613704	20.531759347953727	21.143709979352227
12	23.30191789021964	24.215664399209142	27.31714452699209	25.165273183579124
13	31.71431787862507	18.078698819578378	23.615308338046436	26.591674963750116
14	23.905307414525435	19.28696089461547	23.245091014005837	33.56264067685326
15	26.67614005971204	26.364030407434548	20.718013984939194	26.24181554791423
16	26.903852026319676	24.64661839640353	22.20703992644189	26.242489650834912
17	25.77189840190421	24.922124260087784	23.351464454890046	25.95451288311797
18	25.330495809439196	23.54425789020616	24.8214806940294	26.303765606325243
19	26.494941194631714	24.006827314380704	24.460970452046677	25.037261038940905
20	26.801860254419925	23.186915931950658	24.181891842882788	25.829331970746626
21	27.07406301379282	24.127222096015174	23.019468766452324	25.77924612373968
22	27.269755091667886	23.51635002928977	23.013334429874085	26.200560449168258
23	25.89566369814211	23.82610032134486	23.53569678311345	26.74253919739958
24	26.679577984607533	23.865602752497043	23.452108020948423	26.002711241946997
25	26.494806374047574	23.076632694126474	23.71696305848584	26.711597873340104
26	26.38593875235683	23.58767011829832	23.655080410366892	26.371310718977952
27	26.3671312808697	23.347689478534203	23.645103687140747	26.64007555345535
28	25.624606913734375	23.763678390889364	24.191464104356523	26.420250591019734
29	25.605664621663106	24.15216390408055	24.193621233702718	26.048550240553624
30	25.22122372599604	24.90790068846131	23.90004941174409	25.97082617379856
31	26.259274813559983	24.009658546647586	23.62171231579295	26.10935432399948
32	25.14302778719649	23.915351548043652	24.054081929120773	26.887538735639076
33	25.291263019455286	23.44570404320191	24.55858055496197	26.70445238238084
34	25.876451764902562	23.240102652392764	24.525212460388026	26.358233122316648
35	25.742103052809895	23.21617199870842	24.827480210023506	26.214244738458177
36	25.052630585532537	23.530775831792447	24.909653356055095	26.506940226619918
37	25.629730095931585	22.175020037709317	25.230526346301502	26.964723520057593
38	24.46980120030766	22.400844516139035	26.58918078294358	26.540173500609725
39	24.617429739937833	21.64510773175827	26.906278796834144	26.831183731469753
40	24.083540226754742	21.65400589031132	27.906310479671415	26.35614340326252
41	23.21617199870842	21.737662062768422	28.774622451806696	26.271543486716464
42	23.51486700286426	21.28560864415657	28.67525968129762	26.524264671681543
43	22.09898122825597	21.038415103141116	29.409492582508513	27.4531110860944
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	31.5
2	33.0
3	109.5
4	186.0
5	186.0
6	251.0
7	316.0
8	359.5
9	403.0
10	570.0
11	737.0
12	737.0
13	1228.5
14	1720.0
15	3036.0
16	4352.0
17	4204.0
18	4056.0
19	4056.0
20	4544.5
21	5033.0
22	5569.5
23	6106.0
24	7370.5
25	8635.0
26	8635.0
27	10447.0
28	12259.0
29	16612.5
30	20966.0
31	24101.5
32	27237.0
33	27237.0
34	32115.5
35	36994.0
36	42166.0
37	47338.0
38	55997.5
39	64657.0
40	64657.0
41	72154.5
42	79652.0
43	81015.0
44	82378.0
45	89697.0
46	97016.0
47	97016.0
48	103469.0
49	109922.0
50	115830.5
51	121739.0
52	128694.5
53	135650.0
54	135650.0
55	125033.5
56	114417.0
57	110055.5
58	105694.0
59	99681.0
60	93668.0
61	93668.0
62	87261.5
63	80855.0
64	72693.0
65	64531.0
66	57006.0
67	49481.0
68	49481.0
69	42946.5
70	36412.0
71	31905.0
72	27398.0
73	22157.5
74	16917.0
75	16917.0
76	13576.5
77	10236.0
78	8599.0
79	6962.0
80	5667.0
81	4372.0
82	4372.0
83	3381.0
84	2390.0
85	1864.0
86	1338.0
87	1080.0
88	822.0
89	822.0
90	629.5
91	437.0
92	257.5
93	78.0
94	51.5
95	25.0
96	25.0
97	15.0
98	5.0
99	2.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1483453.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.01687275407567
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.29812962294433	52.393607372490536
2	7.290595407268636	8.751174737189803
3	2.040511497166694	3.6739535663604577
4	0.9396525680858605	2.2558003444739803
5	0.5402697911195165	1.6212651653245531
6	0.3418499248029482	1.2310058062733327
7	0.2367282140438831	0.9945380969699925
8	0.17751041975005155	0.8522896219729124
9	0.12186134867356171	0.658236335128308
>10	0.8139804126852659	9.54702071596483
>50	0.1020789156163944	4.307817949357469
>100	0.08971360715856413	10.519203887867322
>500	0.006327351719345754	2.5100655623793973
>1k	7.909189649182193E-4	0.6840208382472237
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1894	0.1276750931778762	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1677	0.11304705979899599	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1548	0.10435113212215015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	1.348205841371449E-4	0.0	0.0	0.0	0.0
10	1.348205841371449E-4	6.741029206857245E-5	0.0	6.741029206857245E-5	0.0
11	1.348205841371449E-4	6.741029206857245E-5	0.0	1.348205841371449E-4	0.0
12	1.348205841371449E-4	6.741029206857245E-5	0.0	1.348205841371449E-4	0.0
13	1.348205841371449E-4	6.741029206857245E-5	0.0	1.348205841371449E-4	0.0
14	1.348205841371449E-4	6.741029206857245E-5	0.0	2.696411682742898E-4	0.0
15	1.348205841371449E-4	6.741029206857245E-5	0.0	2.696411682742898E-4	0.0
16	1.348205841371449E-4	6.741029206857245E-5	0.0	8.089235048228694E-4	0.0
17	1.348205841371449E-4	6.741029206857245E-5	0.0	0.0012807955493028766	0.0
18	1.348205841371449E-4	6.741029206857245E-5	0.0	0.0014156161334400214	6.741029206857245E-5
19	1.348205841371449E-4	6.741029206857245E-5	0.0	0.0016178470096457388	6.741029206857245E-5
20	1.348205841371449E-4	6.741029206857245E-5	0.0	0.001820077885851456	6.741029206857245E-5
21	1.348205841371449E-4	6.741029206857245E-5	0.0	0.002291949930331463	6.741029206857245E-5
22	1.348205841371449E-4	6.741029206857245E-5	0.0	0.003437924895497195	6.741029206857245E-5
23	1.348205841371449E-4	6.741029206857245E-5	0.0	0.004853541028937216	6.741029206857245E-5
24	1.348205841371449E-4	6.741029206857245E-5	0.0	0.007415132127542969	6.741029206857245E-5
25	1.348205841371449E-4	6.741029206857245E-5	0.0	0.008965568845120135	6.741029206857245E-5
26	1.348205841371449E-4	6.741029206857245E-5	0.0	0.011796801112000177	6.741029206857245E-5
27	1.348205841371449E-4	6.741029206857245E-5	0.0	0.0315480166880919	6.741029206857245E-5
28	1.348205841371449E-4	6.741029206857245E-5	0.0	0.10219400277595583	6.741029206857245E-5
29	1.348205841371449E-4	1.348205841371449E-4	0.0	0.21402767731771752	6.741029206857245E-5
30	1.348205841371449E-4	1.348205841371449E-4	0.0	0.369408400535777	6.741029206857245E-5
31	1.348205841371449E-4	1.348205841371449E-4	0.0	0.8497067315243557	6.741029206857245E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	895	0.0	24.184357	1
CCGTATA	55	5.144062E-4	20.181818	2
TACTATA	110	1.7553248E-9	20.181818	2
TGCGTTA	65	6.903858E-5	19.923077	37
TTAGAGT	180	0.0	18.5	4
ATGCGTT	70	1.2195003E-4	18.5	36
CTTATAC	1275	0.0	17.701962	37
TAGAGTG	210	0.0	17.61905	5
GGTTAGA	95	3.6078163E-6	17.526316	1
CGGCCTT	490	0.0	16.989796	24
TCTTATA	2300	0.0	16.65	37
GACGGAT	80	3.383394E-4	16.1875	7
GCGCAAG	370	0.0	16.0	1
CGCAAGA	370	0.0	16.0	2
ATTAGAG	210	0.0	15.857144	3
CCCGTAT	70	0.0025929695	15.857143	1
GTCTTAT	70	0.0025929695	15.857143	1
GTATCAA	1380	0.0	15.818842	2
AGACGGA	375	0.0	15.786666	6
ACGGACC	355	0.0	15.633802	8
>>END_MODULE