FastQCFastQC Report
Fri 10 Feb 2017
ERR1632022.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632022.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1886506
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA19270.10214650788282678No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA5600.020.81251
TAGAACT6500.020.7769224
TCTAGAC4650.020.6881713
GGTATCA9950.020.266331
ATACCGC1303.274181E-1119.92307727
GTTCTAG5350.019.3644851
TATACTG1254.129106E-1019.245
CTAGAAC6600.018.7803023
CTAGACA5250.018.676194
TATACTT902.1537053E-618.55
GCAATTA6250.018.35200117
CTTATAC11050.017.91402837
TTATACT1258.587449E-917.764
TGTTATA953.6088695E-617.5263162
TCTAGAA7300.017.4863012
TCTTATA21150.017.14420737
CGACGGT1301.3946192E-817.0769237
ACTGATC5350.016.9439248
CAATTAC6800.016.86764718
TTAGGTA1107.814142E-716.818184
Produced by FastQC (version 0.11.5)