##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632022.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1886506
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.658877840833796	34.0	31.0	34.0	31.0	34.0
2	32.81389616571588	34.0	31.0	34.0	31.0	34.0
3	32.8288179311383	34.0	31.0	34.0	31.0	34.0
4	36.31257202468479	37.0	37.0	37.0	35.0	37.0
5	36.20874569177092	37.0	35.0	37.0	35.0	37.0
6	36.23547075917066	37.0	36.0	37.0	35.0	37.0
7	36.22646575203047	37.0	36.0	37.0	35.0	37.0
8	36.202160502007416	37.0	36.0	37.0	35.0	37.0
9	37.97926113142497	39.0	38.0	39.0	35.0	39.0
10	37.89028235266678	39.0	38.0	39.0	35.0	39.0
11	38.00869968078554	39.0	38.0	39.0	35.0	39.0
12	37.97172285696414	39.0	38.0	39.0	35.0	39.0
13	37.99685450245056	39.0	38.0	39.0	35.0	39.0
14	39.37051353136433	40.0	39.0	41.0	37.0	41.0
15	39.38645305130225	40.0	39.0	41.0	37.0	41.0
16	39.281607373631466	40.0	39.0	41.0	36.0	41.0
17	39.33087517346883	40.0	39.0	41.0	36.0	41.0
18	39.353207463957176	40.0	39.0	41.0	36.0	41.0
19	39.397698708617945	40.0	39.0	41.0	37.0	41.0
20	39.364301518256504	40.0	39.0	41.0	37.0	41.0
21	39.33565755953069	40.0	39.0	41.0	36.0	41.0
22	39.31950176675823	40.0	39.0	41.0	36.0	41.0
23	39.281805623729795	40.0	39.0	41.0	36.0	41.0
24	39.22698788130014	40.0	39.0	41.0	36.0	41.0
25	39.16499072889246	40.0	39.0	41.0	36.0	41.0
26	39.09228011996781	40.0	39.0	41.0	36.0	41.0
27	39.018489737111885	40.0	39.0	41.0	36.0	41.0
28	38.957116489425424	40.0	39.0	41.0	35.0	41.0
29	38.82826187671812	40.0	38.0	41.0	35.0	41.0
30	38.78121511407862	40.0	38.0	41.0	35.0	41.0
31	38.715981820359964	40.0	38.0	41.0	35.0	41.0
32	38.63975624779354	40.0	38.0	41.0	35.0	41.0
33	38.502904310932486	40.0	38.0	41.0	35.0	41.0
34	38.504893702961986	40.0	38.0	41.0	34.0	41.0
35	38.48797406422243	40.0	38.0	41.0	34.0	41.0
36	38.459897291606815	40.0	38.0	41.0	34.0	41.0
37	38.43703757104404	40.0	38.0	41.0	34.0	41.0
38	38.36964260914092	40.0	38.0	41.0	34.0	41.0
39	38.18617433498754	40.0	38.0	41.0	34.0	41.0
40	38.19447910581785	40.0	38.0	41.0	34.0	41.0
41	38.20551220086234	40.0	38.0	41.0	34.0	41.0
42	38.07767852315339	40.0	37.0	41.0	33.0	41.0
43	37.1923444717377	39.0	36.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	0.0
14	3.0
15	5.0
16	3.0
17	9.0
18	27.0
19	48.0
20	120.0
21	248.0
22	392.0
23	743.0
24	1239.0
25	2001.0
26	3018.0
27	4423.0
28	6507.0
29	9159.0
30	12883.0
31	18071.0
32	24363.0
33	33927.0
34	49404.0
35	73391.0
36	118305.0
37	213164.0
38	499588.0
39	815461.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.88827811838393	16.861965983675642	11.318808421494552	27.930947476445873
2	19.664660488755402	19.54252994689654	32.592104133249514	28.200705431098548
3	20.416632653169405	19.96839660197211	28.711756018798773	30.90321472605971
4	15.433293082555794	14.248934273201359	32.329502265033874	37.98827037920897
5	16.985527742821915	33.93421489250498	32.5009832992845	16.579274065388606
6	38.07165203821244	32.62051644680695	14.239551848761678	15.068279666218926
7	32.605038096883874	28.290766104109927	19.199991942776755	19.90420385622945
8	29.211038819913636	31.520069376932806	18.87796805310982	20.390923750043733
9	27.857107266025128	13.05010426682979	17.578422756142835	41.51436571100224
10	17.66217547148008	25.645982573074242	31.393327134925624	25.29851482052005
11	39.13032876651334	19.582763055087025	19.644199382350227	21.64270879604942
12	24.30032027462409	23.225741132018662	27.05710981041142	25.416828782945828
13	32.815055981799155	17.634505270590182	22.581534328541757	26.968904419068902
14	24.595628108259397	18.05342787141944	22.63867700394274	34.712267016378426
15	27.313138680714506	25.234693131111165	20.337968710409616	27.114199477764718
16	27.366729816920802	23.814766557858814	21.61185811229861	27.206645512921774
17	26.21963566508667	24.160591060934873	22.89592505934251	26.723848214635947
18	26.61974040898889	22.92592761433041	23.629397415115562	26.82493456156514
19	26.976060505505945	23.046308890615773	23.219645206535255	26.757985397343028
20	27.207175593398592	23.186515176734133	23.41423774957514	26.19207148029214
21	27.745313293464214	23.063748538303088	23.14665312487742	26.04428504335528
22	27.951885655280183	22.88177191061147	22.206025318763896	26.960317115344452
23	27.013007114740162	23.085587853947985	22.86025064325266	27.041154388059198
24	26.92050807153542	22.839842544895166	22.648907557145325	27.590741826424086
25	27.330578328401817	22.512517850460057	23.12110324589479	27.035800575243336
26	27.4220702187006	22.348086886551116	22.675040524652452	27.554802370095828
27	26.662411887372738	22.88065874161015	23.126828115044425	27.330101255972682
28	26.486425169069168	22.84376514042362	23.29788508491359	27.371924605593623
29	26.524696979495427	23.165046917423005	22.94728985754617	27.362966245535397
30	25.976169702084174	23.45749231648349	23.738593993340068	26.827743988092273
31	27.21661102588595	23.08924540923803	22.636026601558648	27.058116963317367
32	26.291673601886238	23.12693413113979	23.260673435440967	27.320718831533004
33	26.355548299342807	22.85494983848448	23.73218001957057	27.057321842602143
34	26.99122080714294	22.144695007596052	23.565946782040452	27.298137403220558
35	25.934823424892368	22.671541993505453	24.16615690594146	27.227477675660715
36	26.198750494300043	22.138015993588148	24.493693632567297	27.169539879544512
37	26.188307908906733	21.995954425800925	24.470158059396578	27.345579605895765
38	25.57871536056604	21.807616832387495	25.43813801811391	27.175529788932558
39	25.637713317635885	21.80745780824445	25.739170720898848	26.815658153220824
40	25.09093530579813	21.962930412095165	26.109272909813168	26.83686137229354
41	25.163079258693056	21.03263917527959	26.881176100155525	26.923105465871828
42	24.552850613780183	21.136799988974325	27.22366109622763	27.08668830101786
43	23.423249117681046	20.771786572637456	27.623659824034487	28.18130448564701
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	10.0
1	11.5
2	13.0
3	63.0
4	113.0
5	113.0
6	143.5
7	174.0
8	174.0
9	174.0
10	246.0
11	318.0
12	318.0
13	544.5
14	771.0
15	1291.0
16	1811.0
17	1839.0
18	1867.0
19	1867.0
20	2498.0
21	3129.0
22	3648.5
23	4168.0
24	5300.0
25	6432.0
26	6432.0
27	8229.5
28	10027.0
29	12802.0
30	15577.0
31	19559.0
32	23541.0
33	23541.0
34	30162.0
35	36783.0
36	44174.0
37	51565.0
38	58326.5
39	65088.0
40	65088.0
41	72964.0
42	80840.0
43	90036.5
44	99233.0
45	112250.5
46	125268.0
47	125268.0
48	131675.5
49	138083.0
50	146774.5
51	155466.0
52	160453.0
53	165440.0
54	165440.0
55	163019.5
56	160599.0
57	157182.0
58	153765.0
59	150204.5
60	146644.0
61	146644.0
62	137166.5
63	127689.0
64	113874.5
65	100060.0
66	89499.5
67	78939.0
68	78939.0
69	64369.5
70	49800.0
71	41885.0
72	33970.0
73	26637.5
74	19305.0
75	19305.0
76	15565.5
77	11826.0
78	9702.0
79	7578.0
80	6283.5
81	4989.0
82	4989.0
83	3837.5
84	2686.0
85	2083.5
86	1481.0
87	1101.0
88	721.0
89	721.0
90	564.5
91	408.0
92	257.5
93	107.0
94	69.5
95	32.0
96	32.0
97	23.0
98	14.0
99	8.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1886506.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.9255428229815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.23189248719032	46.264824178659445
2	8.54745102925582	9.389467750694557
3	2.5461022398028055	4.195381428119342
4	1.244756726606686	2.734757555657192
5	0.7111681420654555	1.9530648120678185
6	0.4594830150603752	1.5142412412078774
7	0.34793575509555413	1.3377392151303082
8	0.25420726635929985	1.116997767546463
9	0.1914522211806475	0.9464055455711322
>10	1.2277530109901051	13.010851495555686
>50	0.1323522216881135	5.101035998931562
>100	0.1005960623571615	10.412882693528038
>500	0.004267843665993427	1.5640183947806214
>1k	5.819786817263764E-4	0.4583319225499093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA	1927	0.10214650788282678	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	5.300804768179905E-5	0.0	0.0	0.0
5	0.0	5.300804768179905E-5	0.0	5.300804768179905E-5	0.0
6	0.0	5.300804768179905E-5	0.0	5.300804768179905E-5	0.0
7	0.0	5.300804768179905E-5	0.0	5.300804768179905E-5	0.0
8	0.0	5.300804768179905E-5	0.0	5.300804768179905E-5	0.0
9	0.0	5.300804768179905E-5	0.0	1.5902414304539716E-4	0.0
10	0.0	5.300804768179905E-5	0.0	3.180482860907943E-4	0.0
11	0.0	2.120321907271962E-4	0.0	3.180482860907943E-4	0.0
12	0.0	2.120321907271962E-4	0.0	3.180482860907943E-4	0.0
13	0.0	2.120321907271962E-4	0.0	3.180482860907943E-4	0.0
14	0.0	2.120321907271962E-4	0.0	3.7105633377259336E-4	0.0
15	0.0	2.120321907271962E-4	0.0	3.7105633377259336E-4	0.0
16	0.0	2.120321907271962E-4	0.0	6.360965721815886E-4	0.0
17	0.0	2.120321907271962E-4	0.0	9.541448582723829E-4	0.0
18	0.0	2.120321907271962E-4	0.0	0.00111316900131778	0.0
19	0.0	2.120321907271962E-4	0.0	0.0013252011920449763	0.0
20	0.0	2.120321907271962E-4	0.0	0.0017492655734993687	0.0
21	0.0	2.120321907271962E-4	0.0	0.002120321907271962	0.0
22	0.0	2.120321907271962E-4	0.0	0.0028094265271353495	0.0
23	0.0	2.6504023840899526E-4	0.0	0.0035515391946805363	0.0
24	0.0	2.6504023840899526E-4	0.0	0.004558692100634718	0.0
25	0.0	2.6504023840899526E-4	0.0	0.005618853054270699	0.0
26	0.0	2.6504023840899526E-4	0.0	0.00726210253240647	0.0
27	0.0	2.6504023840899526E-4	0.0	0.01834078449790247	0.0
28	0.0	3.180482860907943E-4	0.0	0.06843338955720257	0.0
29	0.0	3.7105633377259336E-4	0.0	0.1501187910348549	0.0
30	0.0	3.7105633377259336E-4	0.0	0.25433261277727187	0.0
31	0.0	3.7105633377259336E-4	0.0	0.560295063996616	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTAGA	560	0.0	20.8125	1
TAGAACT	650	0.0	20.776922	4
TCTAGAC	465	0.0	20.688171	3
GGTATCA	995	0.0	20.26633	1
ATACCGC	130	3.274181E-11	19.923077	27
GTTCTAG	535	0.0	19.364485	1
TATACTG	125	4.129106E-10	19.24	5
CTAGAAC	660	0.0	18.780302	3
CTAGACA	525	0.0	18.67619	4
TATACTT	90	2.1537053E-6	18.5	5
GCAATTA	625	0.0	18.352001	17
CTTATAC	1105	0.0	17.914028	37
TTATACT	125	8.587449E-9	17.76	4
TGTTATA	95	3.6088695E-6	17.526316	2
TCTAGAA	730	0.0	17.486301	2
TCTTATA	2115	0.0	17.144207	37
CGACGGT	130	1.3946192E-8	17.076923	7
ACTGATC	535	0.0	16.943924	8
CAATTAC	680	0.0	16.867647	18
TTAGGTA	110	7.814142E-7	16.81818	4
>>END_MODULE