FastQCFastQC Report
Fri 10 Feb 2017
ERR1632021.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632021.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421698
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT5170.12259958548534733No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT4590.10884566680420585No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCGCA451.3212716E-424.66666833
GGTATCA3800.022.8815781
GTATTAT502.6981713E-422.21
CGAGCCG502.6981713E-422.215
GAATGAT500.007027675418.51
TAGATCT500.007027675418.54
CAAGACG500.007027675418.54
AATACTG609.2236017E-418.55
TTATCCT752.0633583E-417.2666664
CGTCCTT752.0633583E-417.26666628
GAGCCGC650.001577691817.07692316
TCTATGG650.001577691817.0769232
GTATCAA5100.017.0490192
GTCTAGG803.3762155E-416.18751
TCGTCCT803.3762155E-416.187527
TCTTATA5300.016.05660437
AAATCCC1059.3149865E-615.85714415
GTGTTAT700.002588845315.8571421
CATAGAC700.002588845315.8571423
GGATACC855.354155E-415.23529425
Produced by FastQC (version 0.11.5)