##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632021.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	421698
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.79659614226295	34.0	31.0	34.0	31.0	34.0
2	32.953995513376874	34.0	33.0	34.0	31.0	34.0
3	32.952809830731944	34.0	33.0	34.0	31.0	34.0
4	36.3886999701208	37.0	37.0	37.0	35.0	37.0
5	36.3117491664651	37.0	37.0	37.0	35.0	37.0
6	36.35630474889613	37.0	37.0	37.0	35.0	37.0
7	36.34749986957491	37.0	37.0	37.0	35.0	37.0
8	36.33097382486993	37.0	37.0	37.0	35.0	37.0
9	38.1228438361102	39.0	39.0	39.0	37.0	39.0
10	38.05838538480145	39.0	38.0	39.0	37.0	39.0
11	38.163991766619716	39.0	39.0	39.0	37.0	39.0
12	38.14020934412779	39.0	38.0	39.0	37.0	39.0
13	38.15974465138559	39.0	39.0	39.0	37.0	39.0
14	39.56399129234666	41.0	39.0	41.0	37.0	41.0
15	39.594463336321255	41.0	39.0	41.0	37.0	41.0
16	39.47815261158459	41.0	39.0	41.0	37.0	41.0
17	39.53751737025075	41.0	39.0	41.0	37.0	41.0
18	39.55994574316217	41.0	39.0	41.0	37.0	41.0
19	39.62623488847469	41.0	39.0	41.0	37.0	41.0
20	39.61787819719325	41.0	39.0	41.0	37.0	41.0
21	39.59407206104843	41.0	39.0	41.0	37.0	41.0
22	39.55802493727739	41.0	39.0	41.0	37.0	41.0
23	39.521413428567364	41.0	39.0	41.0	37.0	41.0
24	39.480203842560314	41.0	39.0	41.0	37.0	41.0
25	39.42119716005293	41.0	39.0	41.0	37.0	41.0
26	39.363082110894524	41.0	39.0	41.0	37.0	41.0
27	39.30281623341823	40.0	39.0	41.0	36.0	41.0
28	39.24781241552011	40.0	39.0	41.0	36.0	41.0
29	39.12000294049296	40.0	39.0	41.0	36.0	41.0
30	39.08248319887692	40.0	39.0	41.0	36.0	41.0
31	39.031325735478944	40.0	39.0	41.0	35.0	41.0
32	38.97100531660098	40.0	39.0	41.0	35.0	41.0
33	38.8521999155794	40.0	38.0	41.0	35.0	41.0
34	38.86683835351365	40.0	38.0	41.0	35.0	41.0
35	38.845801972027374	40.0	38.0	41.0	35.0	41.0
36	38.82521614994617	40.0	38.0	41.0	35.0	41.0
37	38.80863319247423	40.0	38.0	41.0	35.0	41.0
38	38.758338906041764	40.0	38.0	41.0	35.0	41.0
39	38.593481591091255	40.0	38.0	41.0	35.0	41.0
40	38.6143614624684	40.0	38.0	41.0	35.0	41.0
41	38.63051283145759	40.0	38.0	41.0	35.0	41.0
42	38.5105383473481	40.0	38.0	41.0	35.0	41.0
43	37.72726927801413	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	11.0
19	11.0
20	23.0
21	44.0
22	75.0
23	111.0
24	223.0
25	351.0
26	477.0
27	732.0
28	1078.0
29	1508.0
30	2239.0
31	3121.0
32	4223.0
33	6071.0
34	8996.0
35	13493.0
36	21978.0
37	41557.0
38	101561.0
39	213814.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.43226195049537	19.0195353072578	12.55993625770101	25.988266484545814
2	20.0048375851913	20.692059246190404	33.787212649811	25.5158905188073
3	20.749209149675835	21.370269719088068	29.75636593012061	28.124155201115492
4	15.369292716588648	15.606666382102832	34.71394220508516	34.31009869622336
5	15.952648577892237	34.57711442786069	34.02458631532518	15.445650678921883
6	35.68217065293172	33.719154465992254	15.426679756603068	15.171995124472964
7	29.39710408870803	29.45496540178042	20.986582815190015	20.16134769432153
8	27.16968067195007	31.713690840364432	20.88342842508146	20.23320006260404
9	26.73856646225498	13.537887303235966	20.44520011951681	39.278346114992246
10	17.057941939492245	25.936570721227035	33.864044885202205	23.14144245407851
11	35.8154413822214	21.02926739040735	20.6856565599078	22.469634667463446
12	22.1947934303696	25.57209187617679	28.896271739491297	23.336842953962314
13	32.02789674126982	19.12956665670693	23.243411161542145	25.599125440481103
14	23.618086877338758	21.29509743939976	24.366964035874012	30.71985164738747
15	26.561425475103036	26.725761089689776	22.101835911007402	24.610977524199782
16	25.632324554539032	25.760141143662054	23.18839548681758	25.419138814981334
17	23.470587956309966	25.41866454192337	25.276145488003266	25.8346020137634
18	23.75633747373713	24.427196714236253	26.603398640733417	25.2130671712932
19	24.40016314993194	24.94202011866312	26.12722849053114	24.5305882408738
20	26.139796726567354	24.42387680283046	25.68662881967664	23.749697650925544
21	26.449259896893036	24.512565864670925	24.14737561003372	24.890798628402315
22	26.004866041574775	24.694212445873585	24.98043623635872	24.320485276192915
23	25.339460941242308	24.41178283985222	24.702749360917053	25.546006857988417
24	25.16729982119906	24.97000222908337	25.065093977206438	24.797603972511133
25	25.20713875806857	25.02335794810504	24.546950661373778	25.22255263245261
26	24.83388586144587	24.647022276605533	24.757527899112635	25.761563962835965
27	25.060825519684705	24.260726870888647	25.424592955147997	25.25385465427866
28	24.746382482250333	24.224682118482896	25.362937457611846	25.66599794165493
29	23.88083415145436	24.718400371830075	26.16066474111805	25.240100735597515
30	24.793572651518385	25.527035935669602	25.335666756778547	24.343724656033462
31	25.5431612196406	25.47818581069865	24.245075859975625	24.733577109685132
32	23.867791642360174	25.19717902385119	25.45589497697404	25.47913435681459
33	24.0050936926426	24.64085672685192	25.878235135096684	25.4758144454088
34	24.28515193337412	23.82392138449791	26.381675986132258	25.50925069599571
35	24.171326399461226	24.183894635497442	26.26737617916139	25.377402785879944
36	24.22302216278	24.922574923286334	25.684968863973744	25.16943404995992
37	24.683067029011283	23.921621634439813	26.194812401291923	25.200498935256988
38	23.882019834099285	23.806847554410975	27.012696289761866	25.298436321727873
39	23.781473945809562	23.356525285868084	27.777698732268114	25.08430203605424
40	22.669540761397965	22.877983770375955	28.870423857831906	25.582051610394167
41	22.571366238398095	22.759178369354373	29.223283012961883	25.44617237928565
42	22.551209633434354	22.94106208708602	29.351336738613888	25.156391540865737
43	21.66692751684855	22.30838182775351	29.452831173019554	26.571859482378386
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	12.0
2	21.0
3	48.0
4	75.0
5	75.0
6	95.0
7	115.0
8	107.5
9	100.0
10	162.5
11	225.0
12	225.0
13	392.5
14	560.0
15	830.5
16	1101.0
17	1107.0
18	1113.0
19	1113.0
20	1456.0
21	1799.0
22	1967.5
23	2136.0
24	2721.5
25	3307.0
26	3307.0
27	4319.0
28	5331.0
29	6514.0
30	7697.0
31	9264.5
32	10832.0
33	10832.0
34	12578.0
35	14324.0
36	16407.0
37	18490.0
38	19961.0
39	21432.0
40	21432.0
41	22408.0
42	23384.0
43	24668.0
44	25952.0
45	26769.0
46	27586.0
47	27586.0
48	28716.0
49	29846.0
50	30935.5
51	32025.0
52	33639.5
53	35254.0
54	35254.0
55	35316.0
56	35378.0
57	34745.0
58	34112.0
59	31331.5
60	28551.0
61	28551.0
62	25119.5
63	21688.0
64	18426.5
65	15165.0
66	12497.5
67	9830.0
68	9830.0
69	7815.0
70	5800.0
71	4846.5
72	3893.0
73	2886.0
74	1879.0
75	1879.0
76	1464.0
77	1049.0
78	864.0
79	679.0
80	554.0
81	429.0
82	429.0
83	330.5
84	232.0
85	190.5
86	149.0
87	119.0
88	89.0
89	89.0
90	70.0
91	51.0
92	30.5
93	10.0
94	7.5
95	5.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	421698.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.65425343374463
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.05946408401192	81.42247427927902
2	3.2537010480576836	5.5738666833594674
3	0.5953805508959911	1.5299062978790312
4	0.26969623761231326	0.9240251954628995
5	0.16523972732248052	0.7076742740701302
6	0.11100585724080043	0.5704874297240158
7	0.07359989201770933	0.44129006625067796
8	0.061971598634535476	0.4246504812109444
9	0.04355816374942244	0.335784779721142
>10	0.3325695871028097	5.433541789400506
>50	0.026607150397979687	1.521005505536262
>100	0.006928945150376359	0.9925587366753346
>500	2.771578060150529E-4	0.12273448143055403
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	517	0.12259958548534733	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	459	0.10884566680420585	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.3713652898519794E-4	0.0
10	0.0	0.0	0.0	2.3713652898519794E-4	0.0
11	0.0	0.0	0.0	2.3713652898519794E-4	0.0
12	0.0	0.0	0.0	2.3713652898519794E-4	0.0
13	0.0	0.0	0.0	4.742730579703959E-4	0.0
14	0.0	0.0	0.0	4.742730579703959E-4	0.0
15	0.0	0.0	0.0	0.0011856826449259898	0.0
16	0.0	0.0	0.0	0.0028456383478223752	0.0
17	0.0	0.0	0.0	0.004742730579703959	0.0
18	0.0	0.0	0.0	0.004742730579703959	0.0
19	0.0	0.0	0.0	0.005454140166659552	0.0
20	0.0	0.0	0.0	0.005928413224629949	0.0
21	0.0	0.0	0.0	0.0071140958695559385	0.0
22	0.0	0.0	0.0	0.00924832463042272	0.0
23	0.0	0.0	0.0	0.010671143804333908	0.0
24	0.0	0.0	0.0	0.013991055210126679	0.0
25	0.0	0.0	0.0	0.01612528397099346	0.0
26	0.0	0.0	0.0	0.019682331905771427	0.0
27	0.0	0.0	0.0	0.03628188893473529	0.0
28	0.0	0.0	0.0	0.08608056002162685	0.0
29	0.0	0.0	0.0	0.15674724565921583	0.0
30	0.0	0.0	0.0	0.2542103590721322	0.0
31	0.0	0.0	0.0	0.5176690427746871	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGCA	45	1.3212716E-4	24.666668	33
GGTATCA	380	0.0	22.881578	1
GTATTAT	50	2.6981713E-4	22.2	1
CGAGCCG	50	2.6981713E-4	22.2	15
GAATGAT	50	0.0070276754	18.5	1
TAGATCT	50	0.0070276754	18.5	4
CAAGACG	50	0.0070276754	18.5	4
AATACTG	60	9.2236017E-4	18.5	5
TTATCCT	75	2.0633583E-4	17.266666	4
CGTCCTT	75	2.0633583E-4	17.266666	28
GAGCCGC	65	0.0015776918	17.076923	16
TCTATGG	65	0.0015776918	17.076923	2
GTATCAA	510	0.0	17.049019	2
GTCTAGG	80	3.3762155E-4	16.1875	1
TCGTCCT	80	3.3762155E-4	16.1875	27
TCTTATA	530	0.0	16.056604	37
AAATCCC	105	9.3149865E-6	15.857144	15
GTGTTAT	70	0.0025888453	15.857142	1
CATAGAC	70	0.0025888453	15.857142	3
GGATACC	85	5.354155E-4	15.235294	25
>>END_MODULE