FastQCFastQC Report
Fri 10 Feb 2017
ERR1632020.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632020.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1599010
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25390.158785748681997No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA25000.15634673954509354No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC23300.14571516125602715No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG21970.1373975147122282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19390.12126253119117453No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG18090.11313250073482968No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT16890.10562785723666518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13650.021.8205131
CGTATTA450.003826452720.55555515
CTATATA759.26949E-619.7333342
CTTATAC10550.019.46445537
CTAGTAC500.00703605518.53
TCGCCAT7750.016.70967913
ACGTTAA803.3836E-416.187520
ATTACGT803.3836E-416.187517
AATTACG803.3836E-416.187516
AGTCGGT1851.8189894E-1116.011
CCGCAAT700.002593088715.8571429
TATGGGC3500.015.3285724
CGCTAAG855.365781E-415.23529435
TTACGGC855.365781E-415.23529418
TCTTATA17650.015.09348437
AGGTCGC8900.014.96629110
GTCGCCA8550.014.92982512
TAATACT1252.9603125E-614.8000014
ATTTACG750.004105339314.822
ATAGCGT750.004105339314.86
Produced by FastQC (version 0.11.5)