##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632020.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1599010
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.648371805054374	34.0	31.0	34.0	31.0	34.0
2	32.8082488539784	34.0	31.0	34.0	31.0	34.0
3	32.81034327490134	34.0	31.0	34.0	31.0	34.0
4	36.30540146715781	37.0	37.0	37.0	35.0	37.0
5	36.203606606587826	37.0	35.0	37.0	35.0	37.0
6	36.23431873471711	37.0	36.0	37.0	35.0	37.0
7	36.22755079705568	37.0	36.0	37.0	35.0	37.0
8	36.20595868693754	37.0	36.0	37.0	35.0	37.0
9	37.96492642322437	39.0	38.0	39.0	35.0	39.0
10	37.89114702222	39.0	38.0	39.0	35.0	39.0
11	37.997957486194586	39.0	38.0	39.0	35.0	39.0
12	37.966784447877124	39.0	38.0	39.0	35.0	39.0
13	37.98849350535644	39.0	38.0	39.0	35.0	39.0
14	39.36167878875054	40.0	39.0	41.0	37.0	41.0
15	39.389453474337245	40.0	39.0	41.0	37.0	41.0
16	39.263717550234205	40.0	39.0	41.0	36.0	41.0
17	39.32231630821571	40.0	39.0	41.0	36.0	41.0
18	39.3308240723948	40.0	39.0	41.0	36.0	41.0
19	39.39981801239517	40.0	39.0	41.0	37.0	41.0
20	39.378817518339474	40.0	39.0	41.0	37.0	41.0
21	39.34817168122776	40.0	39.0	41.0	37.0	41.0
22	39.33380028892878	40.0	39.0	41.0	36.0	41.0
23	39.290884985084524	40.0	39.0	41.0	36.0	41.0
24	39.243226746549425	40.0	39.0	41.0	36.0	41.0
25	39.181675536738354	40.0	39.0	41.0	36.0	41.0
26	39.095392148892124	40.0	39.0	41.0	36.0	41.0
27	39.031346270504876	40.0	39.0	41.0	36.0	41.0
28	38.96507839225521	40.0	39.0	41.0	35.0	41.0
29	38.83796411529634	40.0	38.0	41.0	35.0	41.0
30	38.79332024189968	40.0	38.0	41.0	35.0	41.0
31	38.72002676656181	40.0	38.0	41.0	35.0	41.0
32	38.64747625093026	40.0	38.0	41.0	35.0	41.0
33	38.5147660114696	40.0	38.0	41.0	35.0	41.0
34	38.52121562716931	40.0	38.0	41.0	35.0	41.0
35	38.48761358590628	40.0	38.0	41.0	34.0	41.0
36	38.46382073908231	40.0	38.0	41.0	34.0	41.0
37	38.4386795579765	40.0	38.0	41.0	34.0	41.0
38	38.36241424381336	40.0	38.0	41.0	34.0	41.0
39	38.18904572204051	40.0	38.0	41.0	34.0	41.0
40	38.205262631253085	40.0	38.0	41.0	34.0	41.0
41	38.21036141112313	40.0	38.0	41.0	34.0	41.0
42	38.08595693585406	40.0	38.0	41.0	34.0	41.0
43	37.22149204820483	39.0	36.0	41.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	2.0
11	1.0
12	4.0
13	1.0
14	0.0
15	3.0
16	2.0
17	13.0
18	16.0
19	57.0
20	127.0
21	228.0
22	432.0
23	734.0
24	1137.0
25	1737.0
26	2555.0
27	3940.0
28	5605.0
29	7851.0
30	11195.0
31	15221.0
32	20945.0
33	28860.0
34	41301.0
35	61198.0
36	97913.0
37	178538.0
38	422558.0
39	696835.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.649451848331154	17.970431704617233	12.62093420303813	26.759182244013484
2	18.783622366339173	20.63189098254545	34.30972914490841	26.274757506206964
3	19.92889350285489	21.635887205208224	30.380610502748578	28.05460878918831
4	15.361442392480349	15.461942076659934	33.324306914903595	35.852308615956126
5	15.504530928512017	35.62460522448265	33.48453105358941	15.386332793415928
6	35.24762196609152	33.9328084252131	15.55700089430335	15.26256871439203
7	29.978861920813504	30.03858637531973	20.410816692828686	19.57173501103808
8	27.67668745035991	33.1922251893359	19.67955172262838	19.451535637675814
9	27.008961795110725	14.412855454312357	18.66579946341799	39.91238328715893
10	17.367558676931353	26.75568007704767	32.18097447795824	23.69578676806274
11	36.45593210799182	21.144395594774267	21.073914484587338	21.325757812646575
12	22.554142875904464	24.881833134251817	27.847543167334788	24.716480822508927
13	31.218066178447913	18.871114001788605	24.062013370773165	25.848806448990313
14	23.59760101562842	19.95403405857374	23.931182419121832	32.51718250667601
15	26.307652860207252	26.809463355451186	21.439578239035402	25.44330554530616
16	26.1320442023502	25.424543936560745	22.781095803028123	25.662316058060924
17	25.044746436857807	25.805842364963322	23.99084433493224	25.158566863246634
18	24.15907342668276	24.531741515062443	25.574761883915677	25.73442317433912
19	25.839363105921787	24.709601565968946	25.415976135233677	24.03505919287559
20	25.958874553630057	23.9799626018599	25.401342080412252	24.659820764097788
21	25.76788137660177	24.898593504731053	23.920175608657857	25.41334951000932
22	26.090831201806118	24.274332243075403	24.033870957655047	25.600965597463432
23	25.14499596625412	24.866386076384764	24.345939049787056	25.642678907574062
24	26.17288197071938	24.557194764260387	24.177022032382535	25.092901232637693
25	25.895335238678935	23.885779325957937	24.552191668594944	25.66669376676819
26	25.66156559371111	24.18390128892252	24.419171862589977	25.735361254776397
27	25.4299222644011	24.08321398865548	24.435119230023577	26.051744516919843
28	24.858943971582416	24.58708826086141	24.962883284031996	25.591084483524178
29	25.01203869894497	24.944684523548947	24.7791445957186	25.26413218178748
30	24.58471179042032	25.704029368171554	24.470828825335676	25.240430016072445
31	25.47663867017717	24.69540528201825	24.706662247265495	25.121293800539085
32	24.317984252756393	24.895841802115058	25.0544399347096	25.731734010418943
33	24.721421379478553	24.273456701333952	25.194464074646188	25.810657844541314
34	25.06532166778194	24.163638751477475	25.10797305832984	25.663066522410745
35	24.82817493323994	24.35725855373012	25.238929087372814	25.575637425657128
36	24.608351417439543	24.481460403624745	25.451185420979233	25.459002757956483
37	25.4216046178573	22.992038824022366	25.44580709313888	26.140549464981454
38	24.144501910557157	23.707418965484894	26.748488127028597	25.39959099692935
39	24.191593548508138	23.107110024327554	27.084633617050553	25.61666281011376
40	23.687031350648212	22.64826361311061	28.055484330929765	25.609220705311415
41	22.895103845504405	23.034314982395358	28.563798850538767	25.506782321561467
42	23.33700227015466	22.08422711552773	28.46886511028699	26.109905504030618
43	22.137009774798155	22.236321223757198	29.12808550290492	26.498583498539723
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	36.0
2	45.0
3	131.5
4	218.0
5	218.0
6	303.5
7	389.0
8	396.5
9	404.0
10	644.5
11	885.0
12	885.0
13	1546.5
14	2208.0
15	3972.5
16	5737.0
17	5720.0
18	5703.0
19	5703.0
20	6410.5
21	7118.0
22	8189.0
23	9260.0
24	11037.0
25	12814.0
26	12814.0
27	14961.0
28	17108.0
29	23568.0
30	30028.0
31	33439.5
32	36851.0
33	36851.0
34	42061.5
35	47272.0
36	51969.0
37	56666.0
38	67107.0
39	77548.0
40	77548.0
41	86415.0
42	95282.0
43	93926.5
44	92571.0
45	100107.5
46	107644.0
47	107644.0
48	114152.0
49	120660.0
50	127161.5
51	133663.0
52	141650.0
53	149637.0
54	149637.0
55	133523.0
56	117409.0
57	110655.5
58	103902.0
59	94874.0
60	85846.0
61	85846.0
62	80427.0
63	75008.0
64	67798.5
65	60589.0
66	53463.0
67	46337.0
68	46337.0
69	39925.0
70	33513.0
71	29400.0
72	25287.0
73	20836.5
74	16386.0
75	16386.0
76	13200.0
77	10014.0
78	8352.0
79	6690.0
80	5269.5
81	3849.0
82	3849.0
83	2988.5
84	2128.0
85	1681.0
86	1234.0
87	941.5
88	649.0
89	649.0
90	488.5
91	328.0
92	200.0
93	72.0
94	48.0
95	24.0
96	24.0
97	15.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1599010.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.60356468888632
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.5395665862497	48.984479554129706
2	7.7479258827366975	8.771204478163666
3	2.1748380033838237	3.693107508371539
4	0.9628375497632791	2.1800015013165823
5	0.557708043229764	1.5784131651234077
6	0.35239546648116116	1.1968103749822003
7	0.25734431935813445	1.0196624079673908
8	0.19084143730487324	0.8641844513445148
9	0.12921108341420612	0.6582427126701391
>10	0.8693093662982645	9.525882558746265
>50	0.11130218845264285	4.4150471482185
>100	0.09494878339803192	11.074616410219077
>500	0.008217692969641057	3.1242413537149796
>1k	0.0035535969598447814	2.9141063750322207
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2539	0.158785748681997	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2500	0.15634673954509354	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2330	0.14571516125602715	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	2197	0.1373975147122282	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1939	0.12126253119117453	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	1809	0.11313250073482968	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1689	0.10562785723666518	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.253869581803741E-5	0.0
3	0.0	0.0	0.0	1.8761608745411224E-4	0.0
4	0.0	0.0	0.0	1.8761608745411224E-4	0.0
5	0.0	6.253869581803741E-5	0.0	1.8761608745411224E-4	0.0
6	0.0	6.253869581803741E-5	0.0	1.8761608745411224E-4	0.0
7	0.0	6.253869581803741E-5	0.0	1.8761608745411224E-4	0.0
8	0.0	6.253869581803741E-5	0.0	1.8761608745411224E-4	0.0
9	6.253869581803741E-5	6.253869581803741E-5	0.0	2.5015478327214966E-4	0.0
10	6.253869581803741E-5	6.253869581803741E-5	0.0	2.5015478327214966E-4	0.0
11	6.253869581803741E-5	1.2507739163607483E-4	0.0	2.5015478327214966E-4	0.0
12	6.253869581803741E-5	1.2507739163607483E-4	0.0	2.5015478327214966E-4	0.0
13	6.253869581803741E-5	1.2507739163607483E-4	0.0	3.126934790901871E-4	0.0
14	6.253869581803741E-5	1.2507739163607483E-4	0.0	4.3777087072626185E-4	0.0
15	6.253869581803741E-5	1.2507739163607483E-4	0.0	5.003095665442993E-4	0.0
16	6.253869581803741E-5	1.2507739163607483E-4	0.0	0.0011256965247246735	0.0
17	6.253869581803741E-5	1.2507739163607483E-4	0.0	0.001813622178723085	0.0
18	6.253869581803741E-5	1.2507739163607483E-4	0.0	0.0019386995703591597	0.0
19	6.253869581803741E-5	1.8761608745411224E-4	0.0	0.002126315657813272	0.0
20	6.253869581803741E-5	1.8761608745411224E-4	0.0	0.0023764704410854215	0.0
21	6.253869581803741E-5	1.8761608745411224E-4	0.0	0.0028142413118116836	0.0
22	6.253869581803741E-5	1.8761608745411224E-4	0.0	0.0043151700114445815	0.0
23	6.253869581803741E-5	1.8761608745411224E-4	0.0	0.005691021319441404	0.0
24	6.253869581803741E-5	1.8761608745411224E-4	0.0	0.007692259585618601	0.0
25	6.253869581803741E-5	1.8761608745411224E-4	0.0	0.009068110893615424	0.0
26	6.253869581803741E-5	1.8761608745411224E-4	0.0	0.011757274813791033	0.0
27	6.253869581803741E-5	1.8761608745411224E-4	0.0	0.02682910050593805	0.0
28	6.253869581803741E-5	3.752321749082245E-4	0.0	0.07667244107291386	0.0
29	6.253869581803741E-5	3.752321749082245E-4	0.0	0.15434550127891633	0.0
30	6.253869581803741E-5	3.752321749082245E-4	0.0	0.25603342067904516	0.0
31	6.253869581803741E-5	3.752321749082245E-4	0.0	0.571728757168498	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1365	0.0	21.820513	1
CGTATTA	45	0.0038264527	20.555555	15
CTATATA	75	9.26949E-6	19.733334	2
CTTATAC	1055	0.0	19.464455	37
CTAGTAC	50	0.007036055	18.5	3
TCGCCAT	775	0.0	16.709679	13
ACGTTAA	80	3.3836E-4	16.1875	20
ATTACGT	80	3.3836E-4	16.1875	17
AATTACG	80	3.3836E-4	16.1875	16
AGTCGGT	185	1.8189894E-11	16.0	11
CCGCAAT	70	0.0025930887	15.857142	9
TATGGGC	350	0.0	15.328572	4
CGCTAAG	85	5.365781E-4	15.235294	35
TTACGGC	85	5.365781E-4	15.235294	18
TCTTATA	1765	0.0	15.093484	37
AGGTCGC	890	0.0	14.966291	10
GTCGCCA	855	0.0	14.929825	12
TAATACT	125	2.9603125E-6	14.800001	4
ATTTACG	75	0.0041053393	14.8	22
ATAGCGT	75	0.0041053393	14.8	6
>>END_MODULE