FastQCFastQC Report
Fri 10 Feb 2017
ERR1632019.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632019.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166601
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG2210.132652264992407No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG1910.11464517019705764No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC1830.10984327825163114No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA1800.10804256877209621No Hit
CGCCTGGAGTCCAGATACTTGCTGTAGTCACTGGTGAATGTGC1710.1026404403334914No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGACTT250.0054830329.610
TAGAACA652.9504008E-928.4615384
GTAGAAC659.291398E-825.6153853
GGTATCA809.640644E-1025.43751
CTGTAGA701.9024628E-723.7857151
TGTAGAA855.1139068E-821.7647082
AGAACAG1101.722583E-920.1818185
GTATCAA1052.2249878E-819.380952
AGAGCAG1152.9958755E-919.30434810
GAACAGA1103.7931386E-818.56
TCAACGG500.007010276418.53
ATCAACG1800.017.4722232
GAAGAGA1107.70955E-716.81818219
CTTATAC1251.6327613E-716.27999937
CAACGCA1951.8189894E-1216.1282064
TAATTTG700.002580038715.8571434
TATTCTG700.002580038715.8571435
TCATCTC700.002580038715.8571435
AACAGAG1059.247498E-615.8571427
AACGCAG2003.6379788E-1215.7255
Produced by FastQC (version 0.11.5)