FastQCFastQC Report
Fri 10 Feb 2017
ERR1632018.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632018.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292829
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT3350.11440123758234327No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC2950.1007413883187799No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACA251.2313796E-437.01
TATTGAG1054.0017767E-1122.9047645
GCTTTAT1059.731593E-1021.1428591
TACAGAA450.00381910320.5555572
GGTATCA2400.020.0416681
CTCAATA609.2144206E-418.532
TAGACTC500.007022671418.55
GGGTAGA500.007022671418.51
GTCTAGG500.007022671418.51
GCCGATC500.007022671418.517
ATAAAGC500.007022671418.536
CTTAGAA500.007022671418.52
GACTATG500.007022671418.57
TTTATTG1351.1405064E-917.8148163
AGGGCTT752.060644E-417.2666665
GATCAGA650.001576134817.0769231
GTTTTGG650.001576134817.0769231
TCTTATA1951.8189894E-1216.12820637
CTTATAC1151.2344026E-616.08695637
ACAGTCT700.002586311215.8571438
Produced by FastQC (version 0.11.5)