FastQCFastQC Report
Fri 10 Feb 2017
ERR1632017.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632017.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170420
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10510.6167116535617886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10120.5938270156084967No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGG7520.44126276258655084No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT6830.4007745569768807No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3620.2124163830536322No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCC3340.19598638657434575No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT3280.19246567304307005RNA PCR Primer, Index 46 (95% over 21bp)
CCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC3200.1877713883347025No Hit
TGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTG3200.1877713883347025No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT2840.16664710714704845No Hit
CCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC2580.1513906818448539No Hit
CCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCACG2510.14728318272503227No Hit
GGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC2430.1425888980166647No Hit
GGCCGGTGGTGCGCCCTCGGCGGACTGGAGAGGCCTCGGGATC2390.14024175566248093No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC2300.13496068536556743No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGGG2290.13437389977702147No Hit
CTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGT2230.1308531862457458No Hit
GGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCG2210.12967961506865391No Hit
CACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC1970.11559676094355122No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC1960.11500997535500528No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC1920.1126628330008215No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT1850.10855533388099989No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA1840.10796854829245392No Hit
TAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGA1830.10738176270390798No Hit
TCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC1740.10210069240699447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCTA200.001837540137.029
ATCTATC303.5867386E-430.83333231
AATCTAT303.5867386E-430.83333230
GTGCATG250.005483377329.61
TAGCGGG250.005483377329.628
CCAATCT250.005483377329.628
TTGGTAA250.005483377329.633
TTCGACC250.005483377329.632
GTACTAT453.981975E-628.7777771
GGCCTAA453.981975E-628.77777710
CTATATC405.9109352E-527.7500024
AATACTG358.8383E-426.428575
TGTCCCC358.8383E-426.4285710
GGTATCA5400.025.6944451
TTACACC451.3167615E-424.66666624
GCCTAAA551.8903584E-523.54545411
AATTGGA400.001924480523.12500221
ACAAGGT400.001924480523.1250025
TAACTGA400.001924480523.1250021
TCTATCA400.001924480523.12500232
Produced by FastQC (version 0.11.5)