##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632017.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	170420
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.67316629503579	34.0	31.0	34.0	31.0	34.0
2	32.82579509447248	34.0	31.0	34.0	31.0	34.0
3	32.80434221335524	34.0	31.0	34.0	31.0	34.0
4	36.28845205961741	37.0	37.0	37.0	35.0	37.0
5	36.21293862222744	37.0	35.0	37.0	35.0	37.0
6	36.265579157375896	37.0	36.0	37.0	35.0	37.0
7	36.25164886750382	37.0	36.0	37.0	35.0	37.0
8	36.255715291632434	37.0	36.0	37.0	35.0	37.0
9	38.02264992371787	39.0	38.0	39.0	35.0	39.0
10	37.95958807651684	39.0	38.0	39.0	35.0	39.0
11	38.06046238704378	39.0	38.0	39.0	35.0	39.0
12	38.01297969721864	39.0	38.0	39.0	35.0	39.0
13	38.06071470484685	39.0	38.0	39.0	35.0	39.0
14	39.39177913390447	40.0	39.0	41.0	37.0	41.0
15	39.401584321089075	40.0	39.0	41.0	37.0	41.0
16	39.25015256425302	40.0	39.0	41.0	36.0	41.0
17	39.31527402886985	40.0	39.0	41.0	36.0	41.0
18	39.362052575988734	40.0	39.0	41.0	37.0	41.0
19	39.398362868207954	40.0	39.0	41.0	37.0	41.0
20	39.360820326252785	40.0	39.0	41.0	37.0	41.0
21	39.34917263232015	40.0	39.0	41.0	36.0	41.0
22	39.31100222978524	40.0	39.0	41.0	36.0	41.0
23	39.2397899307593	40.0	39.0	41.0	36.0	41.0
24	39.12816570825021	40.0	39.0	41.0	36.0	41.0
25	39.04142706255134	40.0	39.0	41.0	35.0	41.0
26	38.88161013965497	40.0	38.0	41.0	35.0	41.0
27	38.804142706255135	40.0	38.0	41.0	35.0	41.0
28	38.64096936979228	40.0	38.0	41.0	35.0	41.0
29	38.49975355005281	40.0	38.0	41.0	35.0	41.0
30	38.353374017134136	40.0	38.0	41.0	34.0	41.0
31	38.2515725853773	40.0	38.0	41.0	34.0	41.0
32	38.07318389860345	40.0	37.0	41.0	34.0	41.0
33	37.860972890505806	40.0	37.0	41.0	33.0	41.0
34	37.85829128036615	40.0	37.0	41.0	33.0	41.0
35	37.65115009975355	40.0	37.0	41.0	33.0	41.0
36	37.644138011970426	40.0	37.0	41.0	33.0	41.0
37	37.491720455345614	40.0	36.0	41.0	33.0	41.0
38	37.378359347494424	40.0	36.0	41.0	33.0	41.0
39	37.11652388217345	40.0	35.0	41.0	32.0	41.0
40	37.00462387043774	40.0	35.0	41.0	31.0	41.0
41	36.958203262527874	40.0	35.0	41.0	31.0	41.0
42	36.73219105738763	39.0	35.0	41.0	31.0	41.0
43	35.79256542659312	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	0.0
17	3.0
18	7.0
19	6.0
20	26.0
21	47.0
22	88.0
23	131.0
24	226.0
25	353.0
26	500.0
27	717.0
28	930.0
29	1278.0
30	1753.0
31	2200.0
32	2790.0
33	3820.0
34	5334.0
35	7793.0
36	11687.0
37	21743.0
38	42114.0
39	66872.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.53784767046122	18.63513672104213	13.437976763290694	25.389038845205963
2	21.52094824551109	18.893909165590895	33.16042717990847	26.424715408989556
3	20.17779603332942	22.463325900715876	29.055275202441027	28.30360286351367
4	15.166060321558502	15.95822086609553	32.43750733481986	36.43821147752611
5	14.929585729374487	35.56742166412393	33.23846966318507	16.26452294331651
6	33.14399718342918	33.586433517192816	15.233540664241286	18.036028635136724
7	29.038258420373197	29.931932871728673	20.821499823964324	20.20830888393381
8	28.5453585259946	30.028752493838752	18.54125102687478	22.88463795329187
9	27.29491843680319	12.96385400774557	19.257129444900833	40.484098110550406
10	20.15080389625631	24.32989085788053	29.938387513202674	25.580917732660485
11	36.427649336932284	21.12310761647694	18.606971012791927	23.84227203379885
12	21.833118178617532	26.927003872784883	25.868442671047998	25.37143527754958
13	32.68161013965497	17.798380471775612	23.153972538434456	26.36603685013496
14	24.363337636427648	20.16371317920432	24.214880882525524	31.258068301842506
15	29.4431404764699	24.603919727731487	20.420725266987443	25.53221452881117
16	27.299612721511558	22.668114071118413	23.47670461213473	26.5555685952353
17	23.3575871376599	25.969369792277902	24.40675976998005	26.26628330008215
18	24.136251613660367	22.47212768454407	25.190705316277434	28.200915385518133
19	26.051519774674336	24.345147283182726	26.832531393028987	22.770801549113955
20	26.53972538434456	22.415796268043657	26.524468959042363	24.520009388569417
21	27.110667761999764	22.608261941086727	24.80988146931111	25.471188827602393
22	27.385283417439265	22.357704494777607	24.14916089660838	26.107851191174746
23	25.958807651684072	22.340687712709776	25.267574228376947	26.4329304072292
24	23.973125220044594	24.988264288229082	24.48773618119939	26.550874310526932
25	26.266870085670696	22.83476117826546	24.950123224973595	25.948245511090246
26	23.804130970543362	24.304072291984507	25.4048820560967	26.486914681375424
27	25.311583147517897	23.950240582091304	24.611547940382582	26.126628330008216
28	25.304541720455347	23.178617533153385	25.969369792277902	25.547470954113365
29	25.220631381293273	23.405703555920667	26.59781715761061	24.77584790517545
30	25.05809177326605	23.467316042717993	26.101983335289287	25.372608848726674
31	24.768806478112896	24.1749794625044	25.217697453350546	25.838516606032158
32	24.998826428822905	23.4878535383171	25.935923013730783	25.57739701912921
33	25.12146461682901	23.42858819387396	26.15362046708133	25.2963267222157
34	25.718225560380237	23.436216406525055	25.287524938387513	25.558033094707195
35	25.443023119352187	23.100575049876777	27.019715995775144	24.43668583499589
36	24.623283652153503	23.910925947658725	25.251144231897666	26.214646168290106
37	25.178969604506513	22.33834057035559	26.57258537730313	25.910104447834758
38	24.723037202206314	22.832414035911277	27.24621523295388	25.19833352892853
39	23.743105269334585	22.732660485858467	27.89461330829715	25.629620936509802
40	23.909752376481634	22.46449947189297	28.836404177913387	24.789343973712004
41	22.957399366271563	22.86234010092712	28.217345381997415	25.962915150803894
42	22.550170167820678	22.474474826898252	29.31698157493252	25.65837343034855
43	22.04494777608262	22.12416383053632	29.31111371904706	26.519774674333995
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	21.0
1	24.5
2	28.0
3	52.0
4	76.0
5	76.0
6	105.0
7	134.0
8	139.0
9	144.0
10	220.5
11	297.0
12	297.0
13	655.0
14	1013.0
15	1464.5
16	1916.0
17	1677.5
18	1439.0
19	1439.0
20	1608.0
21	1777.0
22	1227.5
23	678.0
24	642.5
25	607.0
26	607.0
27	811.5
28	1016.0
29	1384.0
30	1752.0
31	2281.5
32	2811.0
33	2811.0
34	3810.5
35	4810.0
36	5609.0
37	6408.0
38	7062.0
39	7716.0
40	7716.0
41	8765.5
42	9815.0
43	10856.0
44	11897.0
45	12792.0
46	13687.0
47	13687.0
48	14249.0
49	14811.0
50	14459.5
51	14108.0
52	13071.0
53	12034.0
54	12034.0
55	11446.0
56	10858.0
57	9710.5
58	8563.0
59	7859.5
60	7156.0
61	7156.0
62	6481.0
63	5806.0
64	5161.0
65	4516.0
66	4063.5
67	3611.0
68	3611.0
69	3186.5
70	2762.0
71	2568.5
72	2375.0
73	2958.5
74	3542.0
75	3542.0
76	3270.5
77	2999.0
78	2999.5
79	3000.0
80	2619.0
81	2238.0
82	2238.0
83	2129.0
84	2020.0
85	1545.5
86	1071.0
87	908.0
88	745.0
89	745.0
90	439.5
91	134.0
92	76.5
93	19.0
94	14.0
95	9.0
96	9.0
97	5.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	170420.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.15256425302195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89704869458993	35.17251496303251
2	9.27757345774474	8.378124633259008
3	3.5828925652055252	4.853303602863514
4	2.1182861375716384	3.8258420373195636
5	1.3866327047784897	3.1305011148926183
6	0.9655746013593419	2.615890153737824
7	0.7173582502696592	2.2673395141415327
8	0.5393182497498342	1.9481281539725386
9	0.4067629208956582	1.652975002933928
>10	2.8681334390310464	24.07053162774322
>50	0.17804000051982483	5.327426358408637
>100	0.05718073009395834	4.704846848961389
>500	0.0025991240951799242	0.8420373195634314
>1k	0.0025991240951799242	1.2105386691702853
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1051	0.6167116535617886	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1012	0.5938270156084967	No Hit
GTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGG	752	0.44126276258655084	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	683	0.4007745569768807	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	362	0.2124163830536322	No Hit
CTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCC	334	0.19598638657434575	No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	328	0.19246567304307005	RNA PCR Primer, Index 46 (95% over 21bp)
CCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC	320	0.1877713883347025	No Hit
TGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGTGGTG	320	0.1877713883347025	No Hit
CCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGT	284	0.16664710714704845	No Hit
CCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCC	258	0.1513906818448539	No Hit
CCACCGGCCCGTCTCGCCCGCCGCGCCGGGGAGGTGGAGCACG	251	0.14728318272503227	No Hit
GGCCTCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC	243	0.1425888980166647	No Hit
GGCCGGTGGTGCGCCCTCGGCGGACTGGAGAGGCCTCGGGATC	239	0.14024175566248093	No Hit
GTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCACC	230	0.13496068536556743	No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGCCCGCCGCGCCGGGG	229	0.13437389977702147	No Hit
CTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGACGGGCCGGT	223	0.1308531862457458	No Hit
GGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCG	221	0.12967961506865391	No Hit
CACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGAGAC	197	0.11559676094355122	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	196	0.11500997535500528	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	192	0.1126628330008215	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	185	0.10855533388099989	No Hit
AGGTGGGATCCCGAGGCCTCTCCAGTCCGCCGAGGGCGCACCA	184	0.10796854829245392	No Hit
TAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGCGA	183	0.10738176270390798	No Hit
TCTCCAGTCCGCCGAGGGCGCACCACCGGCCCGTCTCGCCCGC	174	0.10210069240699447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	5.867855885459453E-4	0.0
15	0.0	0.0	0.0	5.867855885459453E-4	0.0
16	0.0	0.0	0.0	0.0023471423541837812	0.0
17	0.0	0.0	0.0	0.0023471423541837812	0.0
18	0.0	0.0	0.0	0.0023471423541837812	0.0
19	0.0	0.0	0.0	0.0046942847083675625	0.0
20	0.0	0.0	0.0	0.005281070296913508	0.0
21	0.0	0.0	0.0	0.007041427062551344	0.0
22	0.0	0.0	0.0	0.008214998239643235	0.0
23	5.867855885459453E-4	0.0	0.0	0.012909282948010798	0.0
24	5.867855885459453E-4	0.0	0.0	0.01760356765637836	0.0
25	5.867855885459453E-4	0.0	0.0	0.02229785236474592	0.0
26	5.867855885459453E-4	0.0	0.0	0.030512850604389158	0.0
27	5.867855885459453E-4	0.0	0.0	0.06454641474005399	0.0
28	5.867855885459453E-4	0.0	0.0	0.14669639713648633	0.0
29	5.867855885459453E-4	0.0	0.0	0.31627743222626453	0.0
30	5.867855885459453E-4	0.0	0.0	0.47588311231076164	0.0
31	5.867855885459453E-4	0.0	0.0	0.9423776552047882	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATCTA	20	0.0018375401	37.0	29
ATCTATC	30	3.5867386E-4	30.833332	31
AATCTAT	30	3.5867386E-4	30.833332	30
GTGCATG	25	0.0054833773	29.6	1
TAGCGGG	25	0.0054833773	29.6	28
CCAATCT	25	0.0054833773	29.6	28
TTGGTAA	25	0.0054833773	29.6	33
TTCGACC	25	0.0054833773	29.6	32
GTACTAT	45	3.981975E-6	28.777777	1
GGCCTAA	45	3.981975E-6	28.777777	10
CTATATC	40	5.9109352E-5	27.750002	4
AATACTG	35	8.8383E-4	26.42857	5
TGTCCCC	35	8.8383E-4	26.42857	10
GGTATCA	540	0.0	25.694445	1
TTACACC	45	1.3167615E-4	24.666666	24
GCCTAAA	55	1.8903584E-5	23.545454	11
AATTGGA	40	0.0019244805	23.125002	21
ACAAGGT	40	0.0019244805	23.125002	5
TAACTGA	40	0.0019244805	23.125002	1
TCTATCA	40	0.0019244805	23.125002	32
>>END_MODULE