FastQCFastQC Report
Fri 10 Feb 2017
ERR1632016.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632016.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153319
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8110.5289624899718887No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7330.47808816911146035No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT4970.3241607367645236No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT3830.24980596012235926No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT3540.23089114852040518No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.20675845785584301No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC3030.19762716949627898No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA2600.16958106953476085No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG2530.16501542535497885No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC2520.16436319047215284No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG2410.1571886067610668No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG2370.1545796672297628No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC2020.13175144633085267No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA1860.12131568820563661No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG1670.10892322543194254No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.10370534636933453No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGA1570.10240087660368252No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTCTA200.001836965237.021
TATCTTA200.001836965237.026
CTTAGGG200.001836965237.029
ACCCATA200.001836965237.029
GGTGTCT250.00548168129.615
AGGTGTC250.00548168129.614
CCCATAG250.00548168129.630
CTTCGTC358.8342326E-426.4285721
CAAACGC358.8342326E-426.4285710
TTCGTCT358.8342326E-426.4285722
CTCTTCG358.8342326E-426.4285719
CCAAACG358.8342326E-426.428579
GGTATCA2950.025.7118631
TAATACT400.001923603723.1254
TCTTCGT400.001923603723.12520
ATTACTG400.001923603723.1252
TCTAATA400.001923603723.1252
GGTCTTA400.001923603723.12529
CAATACC400.001923603723.1254
CTAGCAG400.001923603723.1259
Produced by FastQC (version 0.11.5)