##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632016.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	153319
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.727646279978345	34.0	31.0	34.0	31.0	34.0
2	32.92093608750383	34.0	31.0	34.0	31.0	34.0
3	32.98462030146296	34.0	33.0	34.0	31.0	34.0
4	36.38185091215048	37.0	37.0	37.0	35.0	37.0
5	36.273690801531444	37.0	37.0	37.0	35.0	37.0
6	36.31682309433273	37.0	37.0	37.0	35.0	37.0
7	36.28218942205467	37.0	37.0	37.0	35.0	37.0
8	36.29116417404236	37.0	37.0	37.0	35.0	37.0
9	38.06903906234713	39.0	38.0	39.0	35.0	39.0
10	38.03204429979324	39.0	38.0	39.0	35.0	39.0
11	38.10402494146192	39.0	38.0	39.0	37.0	39.0
12	38.05300712892727	39.0	38.0	39.0	35.0	39.0
13	38.10077681174545	39.0	38.0	39.0	37.0	39.0
14	39.43074896131595	40.0	39.0	41.0	37.0	41.0
15	39.44667001480573	40.0	39.0	41.0	37.0	41.0
16	39.43451887893868	40.0	39.0	41.0	37.0	41.0
17	39.43462323651993	40.0	39.0	41.0	37.0	41.0
18	39.44685264057292	40.0	39.0	41.0	37.0	41.0
19	39.473966044652	41.0	39.0	41.0	37.0	41.0
20	39.44597864582994	41.0	39.0	41.0	37.0	41.0
21	39.402644160214976	41.0	39.0	41.0	37.0	41.0
22	39.35944664392541	40.0	39.0	41.0	36.0	41.0
23	39.322536671906285	40.0	39.0	41.0	36.0	41.0
24	39.25791976206472	40.0	39.0	41.0	36.0	41.0
25	39.19452905380285	40.0	39.0	41.0	36.0	41.0
26	39.07918131477508	40.0	39.0	41.0	35.0	41.0
27	38.97801968444876	40.0	39.0	41.0	35.0	41.0
28	38.93132618918725	40.0	38.0	41.0	35.0	41.0
29	38.87372080433606	40.0	38.0	41.0	35.0	41.0
30	38.78323625904161	40.0	38.0	41.0	35.0	41.0
31	38.65770713349291	40.0	38.0	41.0	35.0	41.0
32	38.550492763453974	40.0	38.0	41.0	35.0	41.0
33	38.46005387460132	40.0	38.0	41.0	35.0	41.0
34	38.375674247810124	40.0	38.0	41.0	35.0	41.0
35	38.28213072091522	40.0	38.0	41.0	34.0	41.0
36	38.23362401267945	40.0	38.0	41.0	34.0	41.0
37	38.14624410542724	40.0	38.0	41.0	34.0	41.0
38	38.06249714647239	40.0	38.0	41.0	34.0	41.0
39	37.99856508325778	40.0	37.0	41.0	34.0	41.0
40	37.877549423098245	40.0	37.0	41.0	33.0	41.0
41	37.80514482875573	40.0	37.0	41.0	33.0	41.0
42	37.692784325491296	40.0	37.0	41.0	33.0	41.0
43	36.842315694727986	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	8.0
20	21.0
21	28.0
22	42.0
23	76.0
24	126.0
25	178.0
26	303.0
27	401.0
28	616.0
29	846.0
30	1183.0
31	1579.0
32	1984.0
33	2740.0
34	4224.0
35	5867.0
36	9104.0
37	17324.0
38	38568.0
39	68096.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.29233819683144	20.22515148155154	12.826198970773357	25.656311350843662
2	21.243942368525754	20.01382737951591	33.14070663127205	25.601523620686283
3	20.347119404639997	22.378178829760174	29.344047378341887	27.930654387257942
4	15.765821587670152	16.03715129892577	34.61540970134165	33.58161741206243
5	16.011061903612728	34.36560374121929	32.89416184556382	16.729172509604158
6	33.24571644740704	34.00622232078216	15.855177766617315	16.892883465193485
7	28.995101716029975	29.558632654791644	21.660720458651568	19.78554517052681
8	27.62540846209537	31.148781299121442	20.107749202642854	21.118061036140332
9	27.454522922794954	13.201234028398307	21.832910467717635	37.5113325810891
10	19.512258754622717	25.538909071934984	32.00581793515481	22.943014238287493
11	35.77377885324063	21.232202140634886	20.17427716069111	22.819741845433377
12	22.658639829375353	27.950873668625547	26.23745263144163	23.153033870557465
13	32.16952889074414	18.184960768071797	24.003548157762573	25.641962183421498
14	23.798094169672382	21.149368310515985	25.248664549077414	29.80387297073422
15	27.4329991716617	26.4598647264853	21.35677900325465	24.750357098598347
16	25.76784351580691	23.959196185730406	24.30748961315949	25.965470685303195
17	23.963761829910187	26.008518187569706	24.986466126181362	25.041253856338745
18	24.429457536247952	23.347399865639616	26.24136604073859	25.98177655737384
19	24.72491993816813	24.494681024530554	27.141450179038472	23.638948858262836
20	25.759364462330176	23.625251925723493	27.44930504373235	23.166078568213987
21	25.964818450420363	23.920062092760844	25.40846209536978	24.706657361449004
22	26.372465252186615	23.627860865254796	25.71109908100105	24.288574801557537
23	24.796665775278996	22.952145526647055	26.073741675852308	26.177447022221646
24	24.311403022456446	24.62904141039271	25.86763545287929	25.19192011427155
25	24.52011818496077	24.11834149713995	26.60987874953528	24.751661568364
26	25.27671064903893	24.576210384883804	25.34519531173566	24.8018836543416
27	24.12029820178843	24.809710472935514	26.100483306048172	24.969508019227884
28	24.17899934124277	23.652645790802186	26.89360092356459	25.274753944390454
29	23.75635113717152	23.948108192722366	27.750637559597962	24.544903110508155
30	23.35979232841331	25.23105420724111	26.689451405240057	24.719702059105526
31	25.805020904127996	24.578819324415107	25.40846209536978	24.20769767608711
32	22.79691362453447	24.61730118250184	26.589659468167675	25.996125724796016
33	22.99193185449944	24.18682615983668	27.236024237048245	25.58521774861563
34	24.509682426835553	22.554282248123194	27.579099785414723	25.35693553962653
35	24.143778657570163	24.33162230382405	27.294073141619762	24.230525896986023
36	23.7935285254926	24.120950436671254	26.152662096674256	25.93285894116189
37	23.76939583482804	22.926056131334015	27.317553597401496	25.98699443643645
38	23.82157462545412	22.974973747545967	27.649541152759934	25.553910474239984
39	22.838004422152505	22.989322914968138	28.84247875344869	25.330193909430665
40	22.380787769291477	22.85235358957468	29.798002856788784	24.96885578434506
41	22.435575499448863	22.09184771619956	29.744519596397055	25.72805718795453
42	21.35873570790313	23.810486632446075	29.711255617372927	25.119522042277865
43	21.09262387571012	22.16946366725585	30.47110925586522	26.266803201168802
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	82.0
1	61.0
2	40.0
3	75.5
4	111.0
5	111.0
6	147.5
7	184.0
8	166.5
9	149.0
10	216.0
11	283.0
12	283.0
13	589.0
14	895.0
15	1184.5
16	1474.0
17	1272.5
18	1071.0
19	1071.0
20	1211.0
21	1351.0
22	985.5
23	620.0
24	613.5
25	607.0
26	607.0
27	768.0
28	929.0
29	1350.0
30	1771.0
31	2413.5
32	3056.0
33	3056.0
34	3907.5
35	4759.0
36	5303.5
37	5848.0
38	6347.5
39	6847.0
40	6847.0
41	7992.0
42	9137.0
43	9856.0
44	10575.0
45	11059.0
46	11543.0
47	11543.0
48	11829.0
49	12115.0
50	12053.5
51	11992.0
52	12348.0
53	12704.0
54	12704.0
55	12442.5
56	12181.0
57	11570.5
58	10960.0
59	10442.5
60	9925.0
61	9925.0
62	8358.5
63	6792.0
64	5798.5
65	4805.0
66	4086.5
67	3368.0
68	3368.0
69	2682.0
70	1996.0
71	1918.0
72	1840.0
73	1490.0
74	1140.0
75	1140.0
76	894.5
77	649.0
78	503.0
79	357.0
80	327.0
81	297.0
82	297.0
83	258.5
84	220.0
85	258.5
86	297.0
87	240.0
88	183.0
89	183.0
90	165.0
91	147.0
92	81.0
93	15.0
94	9.0
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	153319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.81729596462278
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.63644919727743	38.55816956802484
2	7.556640704922729	7.226762501712117
3	3.317283428587017	4.758705705098521
4	1.860515870309495	3.558593520698674
5	1.2753536207766698	3.0491980772115657
6	0.8579651630679416	2.4615344477853367
7	0.6574550216196309	2.200640494654935
8	0.48558918609250745	1.8575649462884574
9	0.4078403557349992	1.755164069684775
>10	2.7253011062158143	23.800703109203685
>50	0.16368174812106995	5.297451718312799
>100	0.05319656813934773	4.468461182240949
>500	0.0027280291353511655	1.0070506590833492
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	811	0.5289624899718887	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	733	0.47808816911146035	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	497	0.3241607367645236	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	383	0.24980596012235926	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	354	0.23089114852040518	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	317	0.20675845785584301	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	303	0.19762716949627898	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	260	0.16958106953476085	No Hit
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTG	253	0.16501542535497885	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	252	0.16436319047215284	No Hit
CCATGGTAGGCACGGCGACTACCATCGAAAGTTGATAGGGCAG	241	0.1571886067610668	No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCG	237	0.1545796672297628	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	202	0.13175144633085267	No Hit
CGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA	186	0.12131568820563661	No Hit
TATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGG	167	0.10892322543194254	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	159	0.10370534636933453	No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGA	157	0.10240087660368252	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	6.522348828260033E-4	0.0
16	0.0	0.0	0.0	0.0032611744141300167	0.0
17	0.0	0.0	0.0	0.006522348828260033	0.0
18	0.0	0.0	0.0	0.007174583711086036	0.0
19	0.0	0.0	0.0	0.00782681859391204	0.0
20	0.0	0.0	0.0	0.009131288359564046	0.0
21	0.0	0.0	0.0	0.00978352324239005	0.0
22	0.0	0.0	0.0	0.011087993008042056	0.0
23	0.0	0.0	0.0	0.01565363718782408	0.0
24	0.0	0.0	0.0	0.02543716043021413	0.0
25	0.0	0.0	0.0	0.028698334844344146	0.0
26	0.0	0.0	0.0	0.03717738832108219	0.0
27	0.0	0.0	0.0	0.06261454875129632	0.0
28	0.0	0.0	0.0	0.15262296258128477	0.0
29	0.0	0.0	0.0	0.25111042988801124	0.0
30	0.0	0.0	0.0	0.42199596918842414	0.0
31	0.0	0.0	0.0	0.8022489058759841	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCTCTA	20	0.0018369652	37.0	21
TATCTTA	20	0.0018369652	37.0	26
CTTAGGG	20	0.0018369652	37.0	29
ACCCATA	20	0.0018369652	37.0	29
GGTGTCT	25	0.005481681	29.6	15
AGGTGTC	25	0.005481681	29.6	14
CCCATAG	25	0.005481681	29.6	30
CTTCGTC	35	8.8342326E-4	26.42857	21
CAAACGC	35	8.8342326E-4	26.42857	10
TTCGTCT	35	8.8342326E-4	26.42857	22
CTCTTCG	35	8.8342326E-4	26.42857	19
CCAAACG	35	8.8342326E-4	26.42857	9
GGTATCA	295	0.0	25.711863	1
TAATACT	40	0.0019236037	23.125	4
TCTTCGT	40	0.0019236037	23.125	20
ATTACTG	40	0.0019236037	23.125	2
TCTAATA	40	0.0019236037	23.125	2
GGTCTTA	40	0.0019236037	23.125	29
CAATACC	40	0.0019236037	23.125	4
CTAGCAG	40	0.0019236037	23.125	9
>>END_MODULE