FastQCFastQC Report
Fri 10 Feb 2017
ERR1632015.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632015.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2961545
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10800.023.467591
TAACACG1054.803587E-717.6190474
TTATACG904.4497658E-516.4444454
GTATCAA16050.015.7912762
TCTTATA33800.015.54437837
CTTATAC21000.014.53571437
CCGATCG3900.014.2307718
CTCTTAT53700.014.02141537
GGACCGT2102.044544E-913.2142856
TCGCACG4350.013.18390822
CGATCGC4450.012.4719119
GTTATTC4450.012.471913
TAGATAA4700.012.2021284
TATACTG2901.2732926E-1112.1206895
TTAATAC2756.184564E-1112.1090913
TATACAG3103.6379788E-1211.9354845
CTAGATA4700.011.8085113
TAATACC3002.1827873E-1111.7166674
TACACCG1603.767349E-511.56255
ATAGACT1951.962957E-611.3846164
Produced by FastQC (version 0.11.5)