##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632015.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2961545
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.3502202397735	33.0	31.0	34.0	31.0	34.0
2	32.54004008043099	34.0	31.0	34.0	31.0	34.0
3	32.62587399482365	34.0	31.0	34.0	31.0	34.0
4	36.15296644150266	37.0	35.0	37.0	35.0	37.0
5	35.92253671647738	37.0	35.0	37.0	35.0	37.0
6	35.92882735193961	37.0	35.0	37.0	35.0	37.0
7	35.90545678016036	37.0	35.0	37.0	35.0	37.0
8	35.88680266550061	37.0	35.0	37.0	35.0	37.0
9	37.619799800441996	39.0	37.0	39.0	35.0	39.0
10	37.52141567999136	39.0	37.0	39.0	35.0	39.0
11	37.605965467348966	39.0	37.0	39.0	35.0	39.0
12	37.553594829725704	39.0	37.0	39.0	35.0	39.0
13	37.592877366374644	39.0	37.0	39.0	35.0	39.0
14	38.881106989763786	40.0	38.0	41.0	36.0	41.0
15	38.86691372239827	40.0	38.0	41.0	36.0	41.0
16	38.865082245922316	40.0	38.0	41.0	36.0	41.0
17	38.83544197369954	40.0	38.0	41.0	36.0	41.0
18	38.81915149018502	40.0	38.0	41.0	36.0	41.0
19	38.83991193785676	40.0	38.0	41.0	35.0	41.0
20	38.8077034115639	40.0	38.0	41.0	35.0	41.0
21	38.7769981546794	40.0	38.0	41.0	35.0	41.0
22	38.74150992134173	40.0	38.0	41.0	35.0	41.0
23	38.687627910431885	40.0	38.0	41.0	35.0	41.0
24	38.6269849014619	40.0	38.0	41.0	35.0	41.0
25	38.558605390092	40.0	38.0	41.0	34.0	41.0
26	38.453118895711526	40.0	38.0	41.0	34.0	41.0
27	38.36351802859656	40.0	38.0	41.0	34.0	41.0
28	38.28770287130535	40.0	38.0	41.0	34.0	41.0
29	38.213749580033394	40.0	38.0	41.0	34.0	41.0
30	38.1367124254401	40.0	38.0	41.0	34.0	41.0
31	38.062713887514796	40.0	38.0	41.0	33.0	41.0
32	37.97256870991324	40.0	37.0	41.0	33.0	41.0
33	37.88278483021531	40.0	37.0	41.0	33.0	41.0
34	37.80164306130753	40.0	37.0	41.0	33.0	41.0
35	37.772535281415614	40.0	37.0	41.0	33.0	41.0
36	37.73567411604416	40.0	37.0	41.0	33.0	41.0
37	37.67556191109708	40.0	37.0	41.0	33.0	41.0
38	37.62281714442968	40.0	37.0	41.0	33.0	41.0
39	37.56804944716356	40.0	37.0	41.0	33.0	41.0
40	37.49278704189874	39.0	37.0	41.0	33.0	41.0
41	37.4462670666831	39.0	36.0	41.0	32.0	41.0
42	37.38684673033839	39.0	36.0	41.0	32.0	41.0
43	36.465244998809744	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	0.0
13	0.0
14	5.0
15	7.0
16	19.0
17	32.0
18	78.0
19	146.0
20	313.0
21	587.0
22	1120.0
23	1852.0
24	3149.0
25	4968.0
26	7441.0
27	11065.0
28	15831.0
29	22345.0
30	30659.0
31	40910.0
32	54714.0
33	75631.0
34	107413.0
35	156910.0
36	247594.0
37	441936.0
38	916060.0
39	820754.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.220244838420484	17.46331728878001	11.711150767589213	27.605287105210287
2	20.78820345461575	19.332915758497677	32.10243302060242	27.77644776628415
3	21.408015073213473	20.433388653557518	27.677850581368844	30.48074569186016
4	16.43412475582846	14.735383051751704	31.38959563336029	37.440896559059546
5	17.701537542059974	33.33169004691808	31.658205429936064	17.308566981085885
6	37.800371090089804	31.81518430413855	14.402178592592719	15.982266013178931
7	31.834701144166306	27.728094626284594	19.420167513915878	21.017036715633225
8	28.507350048707686	31.149653305960236	18.681499014872305	21.661497630459777
9	27.562944341551454	13.492923457181977	18.175479352837794	40.76865284842878
10	18.768514407175985	24.582574298212588	31.08688201597477	25.562029278636654
11	38.71823659610102	19.775590105840028	19.620873564305118	21.885299733753836
12	23.63982313285802	23.115941172597413	27.091838888147908	26.15239680639666
13	32.49331007970502	17.73766733242277	22.07746294586103	27.691559642011182
14	24.4995770788558	18.887033626029655	22.551033328887456	34.06235596622709
15	27.506318492543585	25.466234684936413	19.879150916160317	27.14829590635969
16	27.60575983143933	23.71873464694948	21.768671419816346	26.906834101794843
17	26.258827740250446	24.064466351178186	22.937149359540378	26.739556549030997
18	26.58828415573628	22.942281815741445	23.75044107045478	26.718992958067496
19	27.02440786819042	23.263499288378195	23.34274846406183	26.36934437936955
20	27.175410132211397	22.93471819607671	23.152813818463	26.737057853248896
21	27.440947208298372	23.051245211536546	22.80947951153874	26.698328068626342
22	27.553253453856012	22.930227296900775	22.54154503814732	26.974974211095898
23	26.978452125495306	22.823593766091683	22.897507888618946	27.30044621979406
24	26.962885926096007	23.32056409745589	22.685861602643214	27.03068837380489
25	27.206036038621733	22.62616303314655	22.82659895426205	27.34120197396967
26	26.972070321403187	23.27379796693955	22.981619391229916	26.772512320427346
27	27.085389551737354	22.859723556454487	22.783783464374167	27.27110342743399
28	26.676413831294138	23.10317756441317	23.281125223489767	26.939283380802927
29	26.440152015248795	23.370099053028063	23.37793280196654	26.8118161297566
30	26.24572647047403	23.5538882576493	23.197013720878797	27.003371550997873
31	27.0155273683162	23.133972301619593	22.72425372567359	27.126246604390612
32	26.349996370137884	23.02254397620161	23.066271152388364	27.561188501272138
33	26.148716295041947	22.89372607878658	23.635534830637386	27.32202279553409
34	26.87877442348504	22.504841223077822	23.4685611733065	27.147823180130644
35	26.53672323061105	22.619578632099124	24.018780737756813	26.824917399533017
36	25.988732232669097	22.733640717936076	23.95070816077419	27.32691888862064
37	25.995451698353396	21.819084295528178	24.672932540278808	27.512531465839622
38	25.40724520478331	21.74722990871319	25.573914966681244	27.271609919822254
39	25.11374974886419	21.42364880493121	26.001023114624296	27.461578331580306
40	24.926854057594937	21.509347317025405	26.444237720514124	27.119560904865537
41	24.320785265798765	21.58684065242973	27.089542789321115	27.00283129245039
42	24.35394363415042	21.752700026506435	27.068742835243093	26.824613504100054
43	23.697023006572582	21.21379212539401	27.449625111217284	27.639559756816123
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	51.0
1	63.5
2	76.0
3	168.5
4	261.0
5	261.0
6	351.0
7	441.0
8	455.5
9	470.0
10	637.0
11	804.0
12	804.0
13	1410.0
14	2016.0
15	2889.0
16	3762.0
17	3989.0
18	4216.0
19	4216.0
20	5421.0
21	6626.0
22	7159.5
23	7693.0
24	10072.0
25	12451.0
26	12451.0
27	15850.5
28	19250.0
29	24417.5
30	29585.0
31	36335.5
32	43086.0
33	43086.0
34	52906.0
35	62726.0
36	74159.5
37	85593.0
38	97781.5
39	109970.0
40	109970.0
41	121171.0
42	132372.0
43	144112.5
44	155853.0
45	167907.5
46	179962.0
47	179962.0
48	191478.0
49	202994.0
50	212474.0
51	221954.0
52	228683.0
53	235412.0
54	235412.0
55	236520.0
56	237628.0
57	240107.0
58	242586.0
59	237755.0
60	232924.0
61	232924.0
62	216621.5
63	200319.0
64	179834.5
65	159350.0
66	140533.0
67	121716.0
68	121716.0
69	104779.0
70	87842.0
71	75722.5
72	63603.0
73	51162.0
74	38721.0
75	38721.0
76	31369.5
77	24018.0
78	19375.0
79	14732.0
80	12192.0
81	9652.0
82	9652.0
83	7344.0
84	5036.0
85	4133.0
86	3230.0
87	2436.0
88	1642.0
89	1642.0
90	1182.0
91	722.0
92	437.0
93	152.0
94	93.0
95	34.0
96	34.0
97	21.0
98	8.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2961545.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.94093996903251
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.43548596404065	48.58825029524542
2	9.563300321101714	11.273398202637711
3	3.0584028540212227	5.407954170599478
4	1.4235205902783215	3.356145666251051
5	0.8163805566952613	2.4059118692030372
6	0.5427038394753061	1.919248465408649
7	0.3767070613205303	1.5542427801042817
8	0.27487379917178106	1.2961056076835076
9	0.21443940823571106	1.1375334259034313
>10	1.1648351277769222	12.311087261817047
>50	0.07605486949230239	3.1055937730182936
>100	0.04864466498907445	5.676268075811967
>500	0.004191590966778874	1.61899723717789
>1k	4.59352434632211E-4	0.3492631691382904
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	3.376615921757056E-5	0.0	0.0	0.0
3	0.0	3.376615921757056E-5	0.0	0.0	0.0
4	0.0	3.376615921757056E-5	0.0	0.0	0.0
5	0.0	3.376615921757056E-5	0.0	0.0	0.0
6	0.0	3.376615921757056E-5	0.0	0.0	0.0
7	0.0	3.376615921757056E-5	0.0	0.0	0.0
8	0.0	3.376615921757056E-5	0.0	0.0	0.0
9	0.0	3.376615921757056E-5	0.0	3.376615921757056E-5	0.0
10	0.0	3.376615921757056E-5	0.0	6.753231843514112E-5	0.0
11	0.0	3.376615921757056E-5	0.0	6.753231843514112E-5	0.0
12	0.0	3.376615921757056E-5	0.0	6.753231843514112E-5	0.0
13	0.0	3.376615921757056E-5	0.0	6.753231843514112E-5	0.0
14	0.0	6.753231843514112E-5	0.0	6.753231843514112E-5	0.0
15	0.0	6.753231843514112E-5	0.0	1.3506463687028224E-4	0.0
16	0.0	6.753231843514112E-5	0.0	3.0389543295813505E-4	0.0
17	0.0	6.753231843514112E-5	0.0	4.3896006982841726E-4	0.0
18	0.0	6.753231843514112E-5	0.0	5.064923882635583E-4	0.0
19	0.0	6.753231843514112E-5	0.0	5.740247066986995E-4	0.0
20	0.0	6.753231843514112E-5	0.0	7.428555027865522E-4	0.0
21	0.0	6.753231843514112E-5	0.0	8.779201396568345E-4	0.0
22	0.0	6.753231843514112E-5	0.0	0.0012493478910501107	0.0
23	0.0	6.753231843514112E-5	0.0	0.001992203393836663	0.0
24	0.0	1.0129847765271167E-4	0.0	0.0034103820809746263	0.0
25	0.0	1.0129847765271167E-4	0.0	0.004524665335154455	0.0
26	0.0	1.0129847765271167E-4	0.0	0.007394788868647952	0.0
27	0.0	1.0129847765271167E-4	0.0	0.026202539552834754	0.0
28	0.0	1.0129847765271167E-4	0.0	0.08883876490142814	0.0
29	0.0	1.0129847765271167E-4	0.0	0.16714248812697427	0.0
30	0.0	1.0129847765271167E-4	0.0	0.2707370646064807	0.0
31	0.0	1.0129847765271167E-4	0.0	0.5954324516426392	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1080	0.0	23.46759	1
TAACACG	105	4.803587E-7	17.619047	4
TTATACG	90	4.4497658E-5	16.444445	4
GTATCAA	1605	0.0	15.791276	2
TCTTATA	3380	0.0	15.544378	37
CTTATAC	2100	0.0	14.535714	37
CCGATCG	390	0.0	14.23077	18
CTCTTAT	5370	0.0	14.021415	37
GGACCGT	210	2.044544E-9	13.214285	6
TCGCACG	435	0.0	13.183908	22
CGATCGC	445	0.0	12.47191	19
GTTATTC	445	0.0	12.47191	3
TAGATAA	470	0.0	12.202128	4
TATACTG	290	1.2732926E-11	12.120689	5
TTAATAC	275	6.184564E-11	12.109091	3
TATACAG	310	3.6379788E-12	11.935484	5
CTAGATA	470	0.0	11.808511	3
TAATACC	300	2.1827873E-11	11.716667	4
TACACCG	160	3.767349E-5	11.5625	5
ATAGACT	195	1.962957E-6	11.384616	4
>>END_MODULE