FastQCFastQC Report
Fri 10 Feb 2017
ERR1632014.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632014.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78707
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTC250.005465672429.636
TGCTAAT250.005465672429.61
GGTATCA453.9462448E-628.7777791
GTATCAA508.9514215E-625.9000022
TTATGGT500.00697822918.53
CAACGCA752.0366115E-417.2666686
TCTTATA1005.739181E-616.65000237
ATCAACG803.3327664E-416.18754
AACGCAG803.3327664E-416.18757
TCAACGC855.285742E-415.2352945
GGAGTGG800.00623014613.8756
ACGCAGA950.001227135413.6315798
CAGAGTA1000.001806270612.95000111
CTCTTAT1501.8898085E-512.33333437
TGTCTCT4800.012.33333437
TATCAAC1050.002606030812.3333333
AGAGTAC1050.002606030812.33333312
AGTACAT1050.002606030812.33333314
GAGTACA1100.003692261411.77272713
CGCAGAG1100.003692261411.7727279
Produced by FastQC (version 0.11.5)