FastQCFastQC Report
Fri 10 Feb 2017
ERR1632013.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632013.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27510
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT670.24354780079970922TruSeq Adapter, Index 12 (95% over 22bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC280.10178117048346055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTA701.16523675E-418.537
GTCTCTT950.001191878613.63157936
TGTCTCT1200.006867157810.79166735
CTGTCTC1600.00533586749.2537
Produced by FastQC (version 0.11.5)