##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632013.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27510
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.84354780079971	34.0	31.0	34.0	31.0	34.0
2	33.03067975281716	34.0	33.0	34.0	31.0	34.0
3	33.11846601235914	34.0	33.0	34.0	31.0	34.0
4	36.49418393311523	37.0	37.0	37.0	35.0	37.0
5	36.357470010905125	37.0	37.0	37.0	35.0	37.0
6	36.36383133406034	37.0	37.0	37.0	35.0	37.0
7	36.35216284987278	37.0	37.0	37.0	35.0	37.0
8	36.34173027989822	37.0	37.0	37.0	35.0	37.0
9	38.14547437295529	39.0	39.0	39.0	37.0	39.0
10	38.11726644856416	39.0	38.0	39.0	37.0	39.0
11	38.18124318429662	39.0	39.0	39.0	37.0	39.0
12	38.14765539803708	39.0	38.0	39.0	37.0	39.0
13	38.16095965103599	39.0	39.0	39.0	37.0	39.0
14	39.59716466739368	41.0	39.0	41.0	37.0	41.0
15	39.5917121046892	41.0	39.0	41.0	37.0	41.0
16	39.59785532533624	41.0	39.0	41.0	37.0	41.0
17	39.56052344601963	41.0	39.0	41.0	37.0	41.0
18	39.58269720101781	41.0	39.0	41.0	37.0	41.0
19	39.59476553980371	41.0	39.0	41.0	37.0	41.0
20	39.567102871683026	41.0	39.0	41.0	37.0	41.0
21	39.53209741912032	41.0	39.0	41.0	37.0	41.0
22	39.47957106506725	40.0	39.0	41.0	37.0	41.0
23	39.447546346782985	40.0	39.0	41.0	37.0	41.0
24	39.42119229371138	41.0	39.0	41.0	37.0	41.0
25	39.35096328607779	40.0	39.0	41.0	36.0	41.0
26	39.282806252271904	40.0	39.0	41.0	36.0	41.0
27	39.194002181025084	40.0	39.0	41.0	36.0	41.0
28	39.13169756452199	40.0	39.0	41.0	36.0	41.0
29	39.05888767720829	40.0	39.0	41.0	35.0	41.0
30	39.0192657215558	40.0	39.0	41.0	35.0	41.0
31	38.948600508905855	40.0	38.0	41.0	35.0	41.0
32	38.852053798618684	40.0	38.0	41.0	35.0	41.0
33	38.804870956015996	40.0	38.0	41.0	35.0	41.0
34	38.70003635041803	40.0	38.0	41.0	35.0	41.0
35	38.69392948018902	40.0	38.0	41.0	35.0	41.0
36	38.657724463831336	40.0	38.0	41.0	35.0	41.0
37	38.58429661941112	40.0	38.0	41.0	35.0	41.0
38	38.55528898582334	40.0	38.0	41.0	35.0	41.0
39	38.49214830970556	40.0	38.0	41.0	35.0	41.0
40	38.41210468920393	40.0	38.0	41.0	34.0	41.0
41	38.348418756815704	40.0	38.0	41.0	34.0	41.0
42	38.283569611050524	40.0	37.0	41.0	34.0	41.0
43	37.45176299527444	39.0	36.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	1.0
22	5.0
23	6.0
24	15.0
25	23.0
26	24.0
27	47.0
28	69.0
29	90.0
30	146.0
31	178.0
32	295.0
33	417.0
34	656.0
35	944.0
36	1550.0
37	2834.0
38	7099.0
39	13109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.14031261359506	16.77935296255907	11.850236277717194	27.23009814612868
2	22.141039621955652	18.86950199927299	30.268993093420576	28.720465285350784
3	22.057433660487096	20.116321337695382	27.65903307888041	30.167211922937113
4	16.88113413304253	15.110868774990912	30.421664849145763	37.58633224282079
5	18.56052344601963	32.06106870229007	32.101054162122864	17.27735368956743
6	39.00763358778626	30.69429298436932	13.900399854598328	16.39767357324609
7	32.30461650308978	26.84478371501272	18.86223191566703	21.988367866230462
8	30.229007633587784	29.287531806615775	18.40421664849146	22.079243911304978
9	27.662668120683385	13.187931661214103	17.986186841148672	41.163213376953834
10	19.12395492548164	23.605961468556888	31.33406034169393	25.93602326426754
11	39.36750272628135	19.323882224645583	18.85496183206107	22.453653217011997
12	24.096692111959285	23.02798982188295	26.165030897855324	26.710287168302436
13	33.653217011995636	17.266448564158487	21.13413304252999	27.946201381315884
14	25.776081424936386	18.72410032715376	21.603053435114504	33.89676481279535
15	28.905852417302796	23.914940021810253	19.265721555797892	27.913486005089062
16	28.469647400945107	22.511813885859688	20.89058524173028	28.127953471464924
17	27.03017084696474	23.478735005452563	22.24645583424209	27.2446383133406
18	27.044711014176663	21.857506361323157	23.049800072700837	28.047982551799343
19	27.739003998545986	22.399127589967286	22.96982915303526	26.892039258451472
20	28.153398764085786	21.828426026899308	22.642675390766996	27.37549981824791
21	28.302435478007997	22.573609596510362	21.893856779352962	27.23009814612868
22	28.098873137041075	22.31188658669575	21.802980734278442	27.78625954198473
23	28.237004725554343	22.231915667030172	21.78480552526354	27.746274082151945
24	28.18247909850963	22.351872046528534	21.893856779352962	27.57179207560887
25	28.320610687022903	21.959287531806616	21.697564521992003	28.02253725917848
26	28.16066884769175	22.42457288258815	21.664849145765178	27.749909123954925
27	27.117411850236277	22.573609596510362	22.482733551435842	27.826245001817522
28	27.688113413304254	21.824790985096328	22.948018902217378	27.539076699382044
29	27.22282806252272	21.78480552526354	23.25336241366776	27.739003998545986
30	27.150127226463106	23.042529989094877	22.5663395129044	27.241003271537622
31	27.78989458378771	22.104689203925844	21.922937113776808	28.18247909850963
32	26.92838967648128	22.41730279898219	22.751726644856415	27.902580879680116
33	26.775717920756087	22.191930207197384	22.842602689930935	28.18974918211559
34	27.182842602689934	22.079243911304978	22.500908760450745	28.237004725554343
35	26.953834969102147	22.159214830970555	23.1697564521992	27.717193747728096
36	26.68847691748455	22.56997455470738	22.90439840058161	27.83715012722646
37	26.990185387131955	21.504907306434024	23.90766993820429	27.59723736822973
38	26.423118865866957	21.417666303162484	24.25299890948746	27.906215921483096
39	25.776081424936386	20.865139949109416	25.561613958560525	27.797164667393677
40	25.12904398400582	20.134496546710288	26.423118865866957	28.313340603416936
41	24.921846601235913	20.603416939294803	26.670301708469644	27.804434750999636
42	24.46019629225736	21.428571428571427	27.124681933842236	26.98655034532897
43	24.10032715376227	20.04362050163577	27.491821155943292	28.364231188658668
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1.0
1	0.5
2	0.0
3	0.5
4	1.0
5	1.0
6	1.0
7	1.0
8	0.5
9	0.0
10	2.0
11	4.0
12	4.0
13	4.5
14	5.0
15	10.0
16	15.0
17	11.5
18	8.0
19	8.0
20	22.5
21	37.0
22	40.5
23	44.0
24	53.5
25	63.0
26	63.0
27	85.0
28	107.0
29	138.5
30	170.0
31	219.5
32	269.0
33	269.0
34	345.5
35	422.0
36	545.0
37	668.0
38	777.0
39	886.0
40	886.0
41	994.0
42	1102.0
43	1246.5
44	1391.0
45	1499.0
46	1607.0
47	1607.0
48	1741.5
49	1876.0
50	1975.0
51	2074.0
52	2176.5
53	2279.0
54	2279.0
55	2350.0
56	2421.0
57	2369.5
58	2318.0
59	2249.0
60	2180.0
61	2180.0
62	2063.0
63	1946.0
64	1767.5
65	1589.0
66	1418.5
67	1248.0
68	1248.0
69	1085.5
70	923.0
71	799.5
72	676.0
73	576.5
74	477.0
75	477.0
76	386.0
77	295.0
78	241.0
79	187.0
80	150.5
81	114.0
82	114.0
83	89.0
84	64.0
85	44.5
86	25.0
87	17.5
88	10.0
89	10.0
90	6.0
91	2.0
92	2.5
93	3.0
94	2.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	27510.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	93.95129043984007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.07676236168072	90.2653580516176
2	3.0178751063994427	5.670665212649945
3	0.5029791843999072	1.4176663031624863
4	0.1625009672676623	0.6106870229007634
5	0.10833397817844154	0.5089058524173028
6	0.019345353246150273	0.10905125408942204
7	0.02321442389538033	0.15267175572519084
8	0.02321442389538033	0.17448200654307525
9	0.011607211947690165	0.09814612868047984
>10	0.05029791843999071	0.7488186114140313
>50	0.003869070649230055	0.24354780079970922
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	67	0.24354780079970922	TruSeq Adapter, Index 12 (95% over 22bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	28	0.10178117048346055	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0036350418029807343	0.0	0.0	0.0	0.0
15	0.0036350418029807343	0.0	0.0	0.0	0.0
16	0.0036350418029807343	0.0	0.0	0.0	0.0
17	0.0036350418029807343	0.0	0.0	0.0	0.0
18	0.0036350418029807343	0.0	0.0	0.0	0.0
19	0.0036350418029807343	0.0	0.0	0.0	0.0
20	0.0036350418029807343	0.0	0.0	0.0	0.0
21	0.0036350418029807343	0.0	0.0	0.0036350418029807343	0.0
22	0.0036350418029807343	0.0	0.0	0.0036350418029807343	0.0
23	0.0036350418029807343	0.0	0.0	0.0036350418029807343	0.0
24	0.0036350418029807343	0.0	0.0	0.0036350418029807343	0.0
25	0.0036350418029807343	0.0	0.0	0.0036350418029807343	0.0
26	0.0036350418029807343	0.0	0.0	0.007270083605961469	0.0
27	0.0036350418029807343	0.0	0.0	0.029080334423845874	0.0
28	0.0036350418029807343	0.0	0.0	0.0945110868774991	0.0
29	0.0036350418029807343	0.0	0.0	0.19629225736095965	0.0
30	0.0036350418029807343	0.0	0.0	0.2653580516175936	0.0
31	0.0036350418029807343	0.0	0.0	0.4580152671755725	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTCTTA	70	1.16523675E-4	18.5	37
GTCTCTT	95	0.0011918786	13.631579	36
TGTCTCT	120	0.0068671578	10.791667	35
CTGTCTC	160	0.0053358674	9.25	37
>>END_MODULE