FastQCFastQC Report
Fri 10 Feb 2017
ERR1632012.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632012.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3758
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT330.8781266631186802RNA PCR Primer, Index 17 (95% over 21bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC280.7450771687067589No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC170.45236828100053217No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC100.2660989888238425RNA PCR Primer, Index 17 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG80.21287919105907396No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG50.13304949441192124No Hit
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA40.10643959552953698TruSeq Adapter, Index 25 (96% over 27bp)

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)