##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632012.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3758
Sequences flagged as poor quality	0
Sequence length	43
%GC	55
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.44491750931346	34.0	31.0	34.0	31.0	34.0
2	32.6673762639702	34.0	31.0	34.0	31.0	34.0
3	32.76051091005854	34.0	31.0	34.0	31.0	34.0
4	36.26583288983502	37.0	37.0	37.0	35.0	37.0
5	36.0691857370942	37.0	35.0	37.0	35.0	37.0
6	36.07211282597126	37.0	35.0	37.0	35.0	37.0
7	36.01436934539649	37.0	35.0	37.0	35.0	37.0
8	35.99680681213411	37.0	35.0	37.0	35.0	37.0
9	37.83794571580628	39.0	38.0	39.0	35.0	39.0
10	37.727514635444386	39.0	37.0	39.0	35.0	39.0
11	37.7879191059074	39.0	37.0	39.0	35.0	39.0
12	37.7365620010644	39.0	37.0	39.0	35.0	39.0
13	37.76051091005854	39.0	37.0	39.0	35.0	39.0
14	39.165247472059605	40.0	38.0	41.0	36.0	41.0
15	39.108834486428954	40.0	38.0	41.0	36.0	41.0
16	38.987759446514104	40.0	38.0	41.0	36.0	41.0
17	38.967003725385844	40.0	38.0	41.0	36.0	41.0
18	39.05268759978712	40.0	38.0	41.0	36.0	41.0
19	38.99361362426823	40.0	38.0	41.0	36.0	41.0
20	38.965141032464075	40.0	38.0	41.0	35.0	41.0
21	38.95290047897818	40.0	38.0	41.0	35.0	41.0
22	38.93214475784992	40.0	38.0	41.0	35.0	41.0
23	38.83049494411921	40.0	38.0	41.0	35.0	41.0
24	38.761309207025015	40.0	38.0	41.0	35.0	41.0
25	38.74534326769558	40.0	38.0	41.0	35.0	41.0
26	38.59473124002129	40.0	38.0	41.0	35.0	41.0
27	38.47179350718467	40.0	38.0	41.0	34.0	41.0
28	38.47924427887174	40.0	38.0	41.0	34.0	41.0
29	38.31798829164449	40.0	38.0	41.0	34.0	41.0
30	38.32756785524215	40.0	38.0	41.0	34.0	41.0
31	38.24241617881852	40.0	38.0	41.0	34.0	41.0
32	38.20276742948377	40.0	38.0	41.0	33.0	41.0
33	38.11734965407131	40.0	38.0	41.0	33.0	41.0
34	37.91883980840873	40.0	37.0	41.0	33.0	41.0
35	37.92735497605109	40.0	37.0	41.0	33.0	41.0
36	37.95050558807876	40.0	37.0	41.0	33.0	41.0
37	37.88823842469399	40.0	37.0	41.0	33.0	41.0
38	37.79403938265035	40.0	37.0	41.0	33.0	41.0
39	37.835284725918044	40.0	37.0	41.0	33.0	41.0
40	37.71633847791379	40.0	37.0	41.0	33.0	41.0
41	37.650079829696644	40.0	37.0	41.0	33.0	41.0
42	37.586216072378924	39.0	36.0	41.0	33.0	41.0
43	36.66684406599255	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	0.0
21	0.0
22	0.0
23	2.0
24	5.0
25	3.0
26	9.0
27	13.0
28	14.0
29	27.0
30	27.0
31	51.0
32	62.0
33	88.0
34	155.0
35	176.0
36	290.0
37	487.0
38	1112.0
39	1236.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.40074507716871	17.589143161255986	12.480042575838212	26.530069185737094
2	20.729111229377327	18.04151144225652	32.30441724321448	28.924960085151675
3	20.463012240553486	20.463012240553486	27.647684938797234	31.426290580095795
4	16.684406599254924	14.741883980840873	29.61681745609367	38.956891963810534
5	18.201170835550826	31.665779670037253	32.517296434273554	17.615753060138374
6	39.409260244811065	29.96274614156466	13.943587014369346	16.684406599254924
7	33.581692389568914	27.88717402873869	16.657796700372536	21.87333688131985
8	31.426290580095795	29.909526343799893	17.535923363491218	21.128259712613094
9	27.354976051091008	12.905800957956359	17.003725385843534	42.7354976051091
10	20.223523150612028	23.390101117615753	30.840872804683343	25.545502927088876
11	39.116551357104846	19.85098456625865	17.48270356572645	23.54976051091006
12	23.390101117615753	23.017562533262375	24.906865353911655	28.685470995210217
13	33.74135178286323	17.216604576902608	20.11708355508249	28.924960085151675
14	25.918041511442258	19.292176689728578	20.7823310271421	34.00745077168707
15	28.658861096327833	24.906865353911655	18.147951037786054	28.286322511974454
16	30.361894624800428	21.979776476849388	20.729111229377327	26.92921766897286
17	25.784992017030333	24.667376263970198	21.87333688131985	27.674294837679618
18	27.035657264502394	21.208089409260246	23.36349121873337	28.392762107503994
19	27.354976051091008	24.58754656732304	21.7935071846727	26.26397019691325
20	29.350718467269825	21.287919105907395	21.953166577967004	27.408195848855776
21	29.882916444917505	21.68706758914316	20.143693453964875	28.286322511974454
22	29.643427354976048	21.660457690260777	20.48962213943587	28.2064928153273
23	27.541245343267697	22.85790313996807	21.633847791378393	27.96700372538584
24	29.24427887174029	22.591804151144228	20.409792442788717	27.754124534326767
25	29.536987759446514	23.49654071314529	20.06386375731772	26.902607770090476
26	29.45715806279936	23.44332091538052	21.341138903672167	25.75838211814795
27	27.62107503991485	22.72485364555615	21.261309207025015	28.392762107503994
28	27.301756253326236	22.937732836615222	21.55401809473124	28.2064928153273
29	26.609898882384247	22.05960617349654	24.401277275146356	26.92921766897286
30	27.142096860031934	22.43214475784992	23.762639701969132	26.66311868014902
31	28.81852048962214	22.299095263437998	20.915380521554017	27.96700372538584
32	28.07344332091538	22.086216072378924	22.19265566790846	27.647684938797234
33	25.997871208089407	22.11282597126131	22.48536455561469	29.40393826503459
34	26.63650878126663	23.283661522086216	22.51197445449707	27.56785524215008
35	26.450239489089945	21.820117083555083	24.45449707291112	27.27514635444385
36	26.157530601383716	22.032996274614156	23.017562533262375	28.791910590739754
37	27.00904736562001	19.611495476317188	25.598722724853644	27.780734433209155
38	25.359233634912187	21.23469930814263	24.58754656732304	28.81852048962214
39	26.742948376796168	19.05268759978712	26.902607770090476	27.301756253326236
40	24.161788185204898	19.478445981905267	28.845130388504526	27.51463544438531
41	24.693986162852582	19.425226184140502	27.301756253326236	28.579031399680684
42	22.85790313996807	18.520489622139436	31.21341138903672	27.408195848855776
43	22.75146354443853	19.50505588078765	28.286322511974454	29.45715806279936
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.5
11	1.0
12	1.0
13	1.5
14	2.0
15	2.5
16	3.0
17	4.5
18	6.0
19	6.0
20	6.5
21	7.0
22	5.5
23	4.0
24	7.0
25	10.0
26	10.0
27	11.0
28	12.0
29	20.0
30	28.0
31	28.0
32	28.0
33	28.0
34	35.5
35	43.0
36	54.5
37	66.0
38	101.0
39	136.0
40	136.0
41	146.5
42	157.0
43	172.0
44	187.0
45	191.0
46	195.0
47	195.0
48	216.5
49	238.0
50	270.5
51	303.0
52	300.5
53	298.0
54	298.0
55	330.0
56	362.0
57	364.0
58	366.0
59	326.5
60	287.0
61	287.0
62	279.0
63	271.0
64	226.0
65	181.0
66	179.0
67	177.0
68	177.0
69	155.5
70	134.0
71	111.5
72	89.0
73	71.5
74	54.0
75	54.0
76	45.5
77	37.0
78	37.5
79	38.0
80	27.0
81	16.0
82	16.0
83	13.0
84	10.0
85	9.5
86	9.0
87	5.5
88	2.0
89	2.0
90	1.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3758.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.60936668440661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	98.07959922070692	93.77328366152209
2	1.5864180350681882	3.0335284725918044
3	0.13915947676036738	0.3991484832357637
4	0.027831895352073477	0.10643959552953698
5	0.027831895352073477	0.13304949441192124
6	0.0	0.0
7	0.0	0.0
8	0.027831895352073477	0.21287919105907396
9	0.0	0.0
>10	0.11132758140829391	2.3416711016498137
>50	0.0	0.0
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	33	0.8781266631186802	RNA PCR Primer, Index 17 (95% over 21bp)
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	28	0.7450771687067589	No Hit
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	17	0.45236828100053217	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	10	0.2660989888238425	RNA PCR Primer, Index 17 (95% over 22bp)
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	8	0.21287919105907396	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	5	0.13304949441192124	No Hit
ATCCTGAGATGTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	4	0.10643959552953698	TruSeq Adapter, Index 25 (96% over 27bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.07982969664715274	0.0
28	0.0	0.0	0.0	0.18626929217668972	0.0
29	0.0	0.0	0.0	0.37253858435337944	0.0
30	0.0	0.0	0.0	0.4523682810005322	0.0
31	0.0	0.0	0.0	0.8515167642362959	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE