FastQCFastQC Report
Fri 10 Feb 2017
ERR1632011.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632011.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57261
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT890.15542865126351268TruSeq Adapter, Index 11 (95% over 23bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA200.001827367837.01
GACGGAG250.005453368629.59999821
CGAGACG250.005453368629.59999818
GCTACAT250.005453368629.59999827
CCACGAG250.005453368629.59999815
CTCTTAT652.6097077E-622.7692337
TCTGTCT609.091526E-418.537
CATCTCC500.00695553418.4999984
TCTCTTA1107.494291E-716.81818437
GTCTCTT2058.367351E-1114.43902437
TGTCTCT3300.014.01515137
CTGTCTC4350.010.63218436
CCTGTCT2952.873965E-68.77966136
Produced by FastQC (version 0.11.5)