##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632011.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	57261
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.59024466914654	34.0	31.0	34.0	31.0	34.0
2	32.77284713854107	34.0	31.0	34.0	31.0	34.0
3	32.86559787639056	34.0	31.0	34.0	31.0	34.0
4	36.294126892649444	37.0	37.0	37.0	35.0	37.0
5	36.14543930423849	37.0	35.0	37.0	35.0	37.0
6	36.174202336668934	37.0	36.0	37.0	35.0	37.0
7	36.159567593999405	37.0	36.0	37.0	35.0	37.0
8	36.13840135519813	37.0	36.0	37.0	35.0	37.0
9	37.87963884668448	39.0	38.0	39.0	35.0	39.0
10	37.853565253837694	39.0	38.0	39.0	35.0	39.0
11	37.93070327098724	39.0	38.0	39.0	35.0	39.0
12	37.89296379734898	39.0	38.0	39.0	35.0	39.0
13	37.904193080805435	39.0	38.0	39.0	35.0	39.0
14	39.286163357258864	40.0	39.0	41.0	36.0	41.0
15	39.27648836031505	40.0	39.0	41.0	36.0	41.0
16	39.27324007614258	40.0	39.0	41.0	36.0	41.0
17	39.25809888056443	40.0	39.0	41.0	36.0	41.0
18	39.23277623513386	40.0	39.0	41.0	36.0	41.0
19	39.2610677424425	40.0	39.0	41.0	36.0	41.0
20	39.23944744241281	40.0	39.0	41.0	36.0	41.0
21	39.22668133633712	40.0	39.0	41.0	36.0	41.0
22	39.17773004313582	40.0	39.0	41.0	36.0	41.0
23	39.14868758841096	40.0	39.0	41.0	36.0	41.0
24	39.125740032482845	40.0	39.0	41.0	36.0	41.0
25	39.062573130053615	40.0	39.0	41.0	36.0	41.0
26	38.966993241473254	40.0	38.0	41.0	36.0	41.0
27	38.87260089764412	40.0	38.0	41.0	35.0	41.0
28	38.82902848361014	40.0	38.0	41.0	35.0	41.0
29	38.77735282303837	40.0	38.0	41.0	35.0	41.0
30	38.69073191177241	40.0	38.0	41.0	35.0	41.0
31	38.641291629555894	40.0	38.0	41.0	35.0	41.0
32	38.57307766193395	40.0	38.0	41.0	35.0	41.0
33	38.52080822898657	40.0	38.0	41.0	34.0	41.0
34	38.44878713260334	40.0	38.0	41.0	34.0	41.0
35	38.42742879097466	40.0	38.0	41.0	34.0	41.0
36	38.398089450061995	40.0	38.0	41.0	34.0	41.0
37	38.336249803531196	40.0	38.0	41.0	34.0	41.0
38	38.30423848692828	40.0	38.0	41.0	34.0	41.0
39	38.23247934894606	40.0	38.0	41.0	34.0	41.0
40	38.176874312359196	40.0	38.0	41.0	34.0	41.0
41	38.125774960269645	40.0	38.0	41.0	34.0	41.0
42	38.080805434763626	40.0	37.0	41.0	34.0	41.0
43	37.20703445626168	39.0	36.0	40.0	32.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	9.0
21	11.0
22	9.0
23	20.0
24	40.0
25	66.0
26	91.0
27	155.0
28	218.0
29	299.0
30	420.0
31	591.0
32	801.0
33	1110.0
34	1539.0
35	2340.0
36	3634.0
37	6490.0
38	15861.0
39	23552.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.746371876146064	18.181659419150904	12.334747908698764	26.737220796004262
2	20.579451983025095	19.859939574928834	32.45315310595344	27.107455336092627
3	21.180209915998674	20.92523707235291	28.69143046750843	29.203122544139994
4	16.342711444089346	15.403153979148113	32.488080892754226	35.76605368400831
5	17.35212448263216	33.555124779518344	32.68542288817869	16.407327849670807
6	36.67068336214876	32.6504951013779	14.568379874609244	16.110441661864098
7	30.900612982658355	27.715198826426363	20.224934946997084	21.1592532439182
8	28.029548907633465	31.221948621225614	19.278391924695693	21.470110546445227
9	26.756431078744697	13.681214089869195	18.901171827247165	40.66118300413895
10	18.10481828818917	25.139274549868144	32.2435863851487	24.512320776793977
11	37.15268681999965	20.36813887288032	19.964722935331203	22.514451371788827
12	23.05408567786102	24.142086236705612	27.372906515778627	25.430921569654743
13	32.80592375264141	18.036709103927627	22.086585983479157	27.0707811599518
14	24.50184244075374	19.87041791096907	22.95803426415885	32.66970538411834
15	27.512617662981786	25.84656223258413	20.249384397757638	26.391435706676447
16	27.09523061071235	24.07746983112415	22.37124744590559	26.456052112257904
17	25.411711286914308	24.824924468661045	23.385899652468524	26.37746459195613
18	25.52697298335691	23.75264141387681	24.290529330608965	26.429856272157316
19	26.283159567594	24.231152093047626	24.40579102705157	25.079897312306805
20	26.559089083320238	23.338747140287456	24.07921622046419	26.022947555928116
21	27.02537503711077	23.693264176315466	23.349225476327693	25.932135310246068
22	27.411327081259497	23.515132463631442	23.09774541136201	25.975795043747052
23	26.518922128499327	23.35970381236793	23.440037722009745	26.681336337122996
24	26.43509544017743	23.523864410331637	23.630394160074047	26.41064598941688
25	26.64990132900229	23.703742512355706	23.122194862122562	26.524161296519445
26	26.21330399399242	23.495922180891007	23.548313861092193	26.742459964024377
27	26.064860900089066	23.825989766158468	23.75264141387681	26.356507919875654
28	25.865772515324565	23.35795742302789	23.892352561079967	26.883917500567577
29	25.64922023715967	23.32477602556714	24.57169801435532	26.454305722917866
30	26.09978868688986	23.92204117986064	24.093187335184506	25.884982798065
31	26.18536176455179	23.799793926057873	23.429559385969508	26.585284923420826
32	25.687640802640544	23.52735718901172	24.163042908786085	26.621959099561654
33	25.389008225493793	23.667068336214875	23.93950507326103	27.004418365030297
34	26.098042297549817	22.93882398141842	24.215434588987268	26.7476991320445
35	25.502523532596356	22.985976493599484	24.809206964600687	26.70229300920347
36	25.23532596357032	23.1326731981628	24.47390021131311	27.158100626953775
37	25.68065524528038	22.184383786521366	24.875569759522186	27.259391208676064
38	25.065926197586492	22.549379158589616	25.785438605682753	26.599256038141146
39	24.718394718918635	22.294406314943853	26.719756902603862	26.267442063533643
40	24.211941810307188	21.136550182497686	28.37533399696128	26.276174010233845
41	23.541328303732033	21.789699795672448	27.818235797488693	26.850736103106826
42	22.90214980527759	22.013237631197498	28.42248650914235	26.66212605438256
43	22.4690452489478	21.686662824610117	28.813677721311187	27.03061420513089
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.5
2	5.0
3	3.0
4	1.0
5	1.0
6	3.0
7	5.0
8	6.0
9	7.0
10	9.5
11	12.0
12	12.0
13	19.5
14	27.0
15	32.5
16	38.0
17	53.5
18	69.0
19	69.0
20	93.5
21	118.0
22	136.0
23	154.0
24	219.0
25	284.0
26	284.0
27	345.0
28	406.0
29	525.5
30	645.0
31	812.0
32	979.0
33	979.0
34	1217.0
35	1455.0
36	1732.5
37	2010.0
38	2235.0
39	2460.0
40	2460.0
41	2744.0
42	3028.0
43	3169.0
44	3310.0
45	3539.0
46	3768.0
47	3768.0
48	3917.0
49	4066.0
50	4259.5
51	4453.0
52	4649.5
53	4846.0
54	4846.0
55	4740.0
56	4634.0
57	4577.5
58	4521.0
59	4336.0
60	4151.0
61	4151.0
62	3777.0
63	3403.0
64	3037.5
65	2672.0
66	2291.5
67	1911.0
68	1911.0
69	1632.5
70	1354.0
71	1148.5
72	943.0
73	773.0
74	603.0
75	603.0
76	498.5
77	394.0
78	316.5
79	239.0
80	199.5
81	160.0
82	160.0
83	111.5
84	63.0
85	50.5
86	38.0
87	25.5
88	13.0
89	13.0
90	12.5
91	12.0
92	7.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	57261.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	90.5974397932275
#Duplication Level	Percentage of deduplicated	Percentage of total
1	94.24214970025253	85.38097483452961
2	3.753108313896332	6.800440090113689
3	1.077548817394992	2.9286949232461885
4	0.42022476241879836	1.5228515045144164
5	0.23131638298282473	1.047833604023681
6	0.0925265531931299	0.502960129931367
7	0.06939491489484742	0.440090113689946
8	0.03469745744742371	0.2514800649656835
9	0.015421092198854982	0.12574003248284174
>10	0.06168436879541993	0.8435060512390633
>50	0.0019276365248568727	0.15542865126351268
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	89	0.15542865126351268	TruSeq Adapter, Index 11 (95% over 23bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0017463893400394685	0.0
22	0.0	0.0	0.0	0.0017463893400394685	0.0
23	0.0	0.0	0.0	0.0017463893400394685	0.0
24	0.0	0.0	0.0	0.003492778680078937	0.0
25	0.0	0.0	0.0	0.003492778680078937	0.0
26	0.0	0.0	0.0	0.006985557360157874	0.0
27	0.0	0.0	0.0	0.029688618780670962	0.0
28	0.0	0.0	0.0	0.08208029898185501	0.0
29	0.0	0.0	0.0	0.16765337664378896	0.0
30	0.0	0.0	0.0	0.2602120116658808	0.0
31	0.0	0.0	0.0	0.48549623653097224	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20	0.0018273678	37.0	1
GACGGAG	25	0.0054533686	29.599998	21
CGAGACG	25	0.0054533686	29.599998	18
GCTACAT	25	0.0054533686	29.599998	27
CCACGAG	25	0.0054533686	29.599998	15
CTCTTAT	65	2.6097077E-6	22.76923	37
TCTGTCT	60	9.091526E-4	18.5	37
CATCTCC	50	0.006955534	18.499998	4
TCTCTTA	110	7.494291E-7	16.818184	37
GTCTCTT	205	8.367351E-11	14.439024	37
TGTCTCT	330	0.0	14.015151	37
CTGTCTC	435	0.0	10.632184	36
CCTGTCT	295	2.873965E-6	8.779661	36
>>END_MODULE