##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632010.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	642935
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.64032289422725	34.0	31.0	34.0	31.0	34.0
2	32.820239993156385	34.0	31.0	34.0	31.0	34.0
3	32.89332358636565	34.0	31.0	34.0	31.0	34.0
4	36.327409458187844	37.0	37.0	37.0	35.0	37.0
5	36.18223148529789	37.0	35.0	37.0	35.0	37.0
6	36.20358823209189	37.0	36.0	37.0	35.0	37.0
7	36.18328757961536	37.0	36.0	37.0	35.0	37.0
8	36.166651372222695	37.0	36.0	37.0	35.0	37.0
9	37.926795088150435	39.0	38.0	39.0	35.0	39.0
10	37.88324169628345	39.0	38.0	39.0	35.0	39.0
11	37.95725228833397	39.0	38.0	39.0	35.0	39.0
12	37.92346504701097	39.0	38.0	39.0	35.0	39.0
13	37.93745090872328	39.0	38.0	39.0	35.0	39.0
14	39.30443824025757	40.0	39.0	41.0	36.0	41.0
15	39.311065659825644	40.0	39.0	41.0	36.0	41.0
16	39.29577017894499	40.0	39.0	41.0	36.0	41.0
17	39.28529011486387	40.0	39.0	41.0	36.0	41.0
18	39.27680247614455	40.0	39.0	41.0	36.0	41.0
19	39.29672828512991	40.0	39.0	41.0	36.0	41.0
20	39.27612589141982	40.0	39.0	41.0	36.0	41.0
21	39.24625973076594	40.0	39.0	41.0	36.0	41.0
22	39.20932287089675	40.0	39.0	41.0	36.0	41.0
23	39.15996173796729	40.0	39.0	41.0	36.0	41.0
24	39.10438224703897	40.0	39.0	41.0	36.0	41.0
25	39.05011237527899	40.0	39.0	41.0	36.0	41.0
26	38.96189350400896	40.0	38.0	41.0	35.0	41.0
27	38.8780094410788	40.0	38.0	41.0	35.0	41.0
28	38.80012754010903	40.0	38.0	41.0	35.0	41.0
29	38.738768304727536	40.0	38.0	41.0	35.0	41.0
30	38.66715297813931	40.0	38.0	41.0	35.0	41.0
31	38.59254668045759	40.0	38.0	41.0	35.0	41.0
32	38.5075287548508	40.0	38.0	41.0	34.0	41.0
33	38.43516685201459	40.0	38.0	41.0	34.0	41.0
34	38.36005972609984	40.0	38.0	41.0	34.0	41.0
35	38.324990862217795	40.0	38.0	41.0	34.0	41.0
36	38.292746545140645	40.0	38.0	41.0	34.0	41.0
37	38.24293902182958	40.0	38.0	41.0	34.0	41.0
38	38.18984811839455	40.0	38.0	41.0	34.0	41.0
39	38.13530916811186	40.0	38.0	41.0	34.0	41.0
40	38.06017249022063	40.0	37.0	41.0	33.0	41.0
41	38.011508161789294	40.0	37.0	41.0	33.0	41.0
42	37.958658340267675	40.0	37.0	41.0	33.0	41.0
43	37.054294757634906	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.0
16	3.0
17	5.0
18	11.0
19	22.0
20	53.0
21	81.0
22	186.0
23	331.0
24	518.0
25	822.0
26	1148.0
27	1741.0
28	2498.0
29	3487.0
30	4824.0
31	6592.0
32	8742.0
33	12081.0
34	17796.0
35	25729.0
36	41543.0
37	75032.0
38	178628.0
39	261060.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75782155272306	17.97009028906499	12.4182071282478	26.85388102996415
2	20.539556875889474	19.911188533833123	32.51495096704954	27.03430362322785
3	21.008189008220114	21.011610816023392	28.952382433683034	29.02781774207346
4	16.129779837775203	15.357851104699542	32.07898154556837	36.43338751195688
5	16.920217440332227	33.972018944372294	32.45740238126716	16.650361234028324
6	36.67244744803129	32.31819701836111	14.952055806574538	16.057299727033058
7	30.80342491853764	28.31779262289345	20.185088694813626	20.693693763755277
8	28.06660082278924	31.317940382775866	19.48517346232512	21.13028533210978
9	27.02730447090297	13.848833863454315	19.02571799637599	40.098143669266726
10	18.53639948050736	25.365550172256917	31.702427150489555	24.395623196746172
11	37.727919618623964	20.364422531049016	20.014464914804762	21.893192935522254
12	23.055207758171512	24.02077970556899	27.256410057004203	25.66760247925529
13	31.779106752626628	18.13744779798891	23.329730066025338	26.75371538335913
14	24.158274164573402	20.12971762308787	22.78410725812096	32.92790095421776
15	27.297316213925203	26.015538118161246	20.398173998926797	26.288971668986754
16	27.07132136219058	24.26575003693997	22.521561277578606	26.141367323290847
17	25.738060612659137	24.753668722343626	23.61793960509227	25.89033105990497
18	25.492779207851495	23.3239752074471	24.79784115034957	26.385404434351837
19	26.262063816715532	23.9308794823738	24.369026417911606	25.43803028299906
20	26.814530240226464	23.178081765652827	24.124678233413952	25.882709760706756
21	27.036947747439477	23.66942225885976	23.44482723759011	25.84880275611065
22	27.09107452541859	23.43860576885688	23.081493463569412	26.388826242155115
23	26.118503425696222	23.419785825938856	23.77176541952141	26.689945328843507
24	26.28181697994354	24.015958067300737	23.59554231765264	26.106682635103084
25	26.536586124569357	23.218365775700498	23.53830480530691	26.70674329442323
26	26.477948781758652	23.840668185741947	23.649824632350082	26.03155840014931
27	26.596467761126707	23.27420345758125	23.546081641223452	26.583247140068593
28	25.807429989034663	23.511552489754017	24.317854837580782	26.363162683630538
29	25.435386158787438	23.669888869014752	24.637171720313873	26.257553251883937
30	25.498378529711403	24.328275797708944	24.298568284507766	25.87477738807189
31	26.4245996873712	23.684509320537845	23.787941238227813	26.102949753863143
32	25.333820681717434	23.493199156990986	24.17989376842138	26.993086392870197
33	25.28218249123162	23.281980293497785	24.617418557085863	26.81841865818473
34	26.18942816925506	23.025655781688663	24.39531212330951	26.38960392574677
35	25.845847558462363	22.982727647429364	24.951044817905387	26.220379976202885
36	25.201147860981283	23.267515378693023	25.036590012987315	26.494746747338375
37	25.833249084277572	21.873595309012575	25.463538304805304	26.829617301904545
38	24.647903753878698	21.805936836538685	26.873012046318834	26.673147363263784
39	24.65256985542862	21.431871028953157	27.273674632738924	26.6418844828793
40	24.119234448272376	21.488486394425564	27.94077161765964	26.45150753964242
41	23.197523855444175	21.59938407459541	28.793423907548977	26.409668162411442
42	23.453070683661643	21.557233623927768	28.452176347531243	26.53751934487934
43	22.310809024240395	21.34025990185633	29.056747571682983	27.292183502220286
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	22.0
2	25.0
3	51.0
4	77.0
5	77.0
6	119.5
7	162.0
8	151.0
9	140.0
10	216.5
11	293.0
12	293.0
13	482.0
14	671.0
15	1019.5
16	1368.0
17	1308.5
18	1249.0
19	1249.0
20	1697.0
21	2145.0
22	2324.0
23	2503.0
24	3116.0
25	3729.0
26	3729.0
27	4569.5
28	5410.0
29	7038.0
30	8666.0
31	10176.0
32	11686.0
33	11686.0
34	13859.0
35	16032.0
36	18610.5
37	21189.0
38	24471.0
39	27753.0
40	27753.0
41	30499.5
42	33246.0
43	34393.0
44	35540.0
45	38477.0
46	41414.0
47	41414.0
48	43861.5
49	46309.0
50	48505.0
51	50701.0
52	52787.5
53	54874.0
54	54874.0
55	52320.0
56	49766.0
57	48661.5
58	47557.0
59	44934.0
60	42311.0
61	42311.0
62	39567.0
63	36823.0
64	33538.0
65	30253.0
66	26770.5
67	23288.0
68	23288.0
69	20032.5
70	16777.0
71	14665.5
72	12554.0
73	9961.5
74	7369.0
75	7369.0
76	5834.0
77	4299.0
78	3560.5
79	2822.0
80	2314.0
81	1806.0
82	1806.0
83	1375.0
84	944.0
85	793.0
86	642.0
87	479.0
88	316.0
89	316.0
90	245.0
91	174.0
92	99.0
93	24.0
94	15.5
95	7.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	642935.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.57763261409298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.11434302321625	59.330219904459504
2	6.117478251636441	8.145744391243094
3	1.6669910037876063	3.3295294386350793
4	0.7991367438884754	2.1281853017211767
5	0.4570864701278098	1.521586754052095
6	0.30799563539177643	1.2303372155914694
7	0.2139091079399414	0.9969093400863642
8	0.1561110692500228	0.8314804332416995
9	0.12153969541574573	0.7282642670476452
>10	0.8967345472678443	11.843324295745246
>50	0.10285658806065454	4.8441434270985475
>100	0.045350334792849514	4.9029676654393555
>500	4.6752922466374795E-4	0.16730756563857924
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	1.5553671833077993E-4
11	0.0	0.0	0.0	0.0	1.5553671833077993E-4
12	0.0	0.0	0.0	0.0	1.5553671833077993E-4
13	0.0	0.0	0.0	0.0	1.5553671833077993E-4
14	0.0	0.0	0.0	0.0	1.5553671833077993E-4
15	0.0	0.0	0.0	0.0	1.5553671833077993E-4
16	0.0	0.0	0.0	7.776835916538997E-4	1.5553671833077993E-4
17	0.0	0.0	0.0	9.332203099846796E-4	1.5553671833077993E-4
18	0.0	0.0	0.0	0.0010887570283154597	1.5553671833077993E-4
19	0.0	0.0	0.0	0.0012442937466462395	1.5553671833077993E-4
20	0.0	0.0	0.0	0.0012442937466462395	1.5553671833077993E-4
21	0.0	0.0	0.0	0.0013998304649770195	4.666101549923398E-4
22	0.0	0.0	0.0	0.0018664406199693593	4.666101549923398E-4
23	0.0	0.0	0.0	0.0038884179582694984	4.666101549923398E-4
24	0.0	0.0	0.0	0.006843615606554317	4.666101549923398E-4
25	0.0	0.0	0.0	0.009021129663185237	4.666101549923398E-4
26	0.0	0.0	0.0	0.015864745269739553	4.666101549923398E-4
27	0.0	0.0	0.0	0.05054943345750348	4.666101549923398E-4
28	0.0	0.0	0.0	0.1387387527510557	4.666101549923398E-4
29	0.0	0.0	0.0	0.24434818449765527	4.666101549923398E-4
30	0.0	0.0	0.0	0.39957382939177366	4.666101549923398E-4
31	0.0	0.0	0.0	0.8033471501784784	4.666101549923398E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACCGA	35	8.865126E-4	26.428572	1
CTAGTGC	35	8.865126E-4	26.428572	3
GGTATCA	470	0.0	23.223406	1
ACCGTCG	75	3.7354584E-7	22.199999	8
TACCGTC	75	3.7354584E-7	22.199999	7
ATTACTC	60	3.723E-5	21.583332	3
AAGACGG	95	7.1304385E-9	21.421053	5
CTCTATG	95	7.1304385E-9	21.421053	1
CCGTCGT	80	6.950304E-7	20.8125	9
TGTCCGA	100	1.2871169E-8	20.35	12
CGTCGTA	85	1.2435612E-6	19.588236	10
ATACCGT	95	1.673252E-7	19.473684	6
GTCCGAG	130	6.9485395E-10	18.5	13
GTATCAA	615	0.0	17.747967	2
CGGACCA	115	6.391929E-8	17.695652	9
GGTTTAA	85	2.7200933E-5	17.411764	1
CTCTAAT	85	2.7200933E-5	17.411764	11
TAGGACA	150	2.5102054E-10	17.266666	4
ACGGACC	120	1.0390613E-7	16.958332	8
CAAGACG	155	4.0017767E-10	16.709679	4
>>END_MODULE