FastQCFastQC Report
Fri 10 Feb 2017
ERR1632009.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632009.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences371385
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT3800.10231969519501326No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCGA308.296831E-637.020
TCGCGGT352.3839879E-531.71428517
GTCATAA303.5948292E-430.8333321
TATAGAG250.005491611529.67
GTCGAAA405.9294704E-527.75000222
TCTTAAT358.8580465E-426.428572
TAGTGGG358.8580465E-426.428577
CGAAATA451.3208567E-424.66666824
GGTCGAA451.3208567E-424.66666821
ATCGCGG551.8981664E-523.54545416
GAATAAG400.001928739423.1250021
GGTATCA1600.023.1250021
ACACTAG400.001928739423.1250026
TTATGTT450.003821004920.5555574
TAATACC555.13409E-420.1818184
TCGAAAT555.13409E-420.18181823
CATCGCG759.2381015E-619.73333415
CCTTATC500.007026134518.52
TACCCAC500.007026134518.529
AGGGTTG500.007026134518.56
Produced by FastQC (version 0.11.5)