##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632009.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	371385
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.85905192724531	34.0	31.0	34.0	31.0	34.0
2	33.037470549429834	34.0	33.0	34.0	31.0	34.0
3	33.12748226234231	34.0	33.0	34.0	31.0	34.0
4	36.49115069267741	37.0	37.0	37.0	35.0	37.0
5	36.364333508353866	37.0	37.0	37.0	35.0	37.0
6	36.38537097621067	37.0	37.0	37.0	35.0	37.0
7	36.36199092585861	37.0	37.0	37.0	35.0	37.0
8	36.34705763560726	37.0	37.0	37.0	35.0	37.0
9	38.16186706517495	39.0	39.0	39.0	37.0	39.0
10	38.13095574673183	39.0	38.0	39.0	37.0	39.0
11	38.19928376213363	39.0	39.0	39.0	37.0	39.0
12	38.167658898447705	39.0	39.0	39.0	37.0	39.0
13	38.18440432435344	39.0	39.0	39.0	37.0	39.0
14	39.62028353326063	41.0	39.0	41.0	37.0	41.0
15	39.6255449196925	41.0	39.0	41.0	37.0	41.0
16	39.62639848136031	41.0	39.0	41.0	37.0	41.0
17	39.60999232602286	41.0	39.0	41.0	37.0	41.0
18	39.60406047632511	41.0	39.0	41.0	37.0	41.0
19	39.62100246375056	41.0	39.0	41.0	37.0	41.0
20	39.603231148269316	41.0	39.0	41.0	37.0	41.0
21	39.584452791577476	41.0	39.0	41.0	37.0	41.0
22	39.55258559177134	41.0	39.0	41.0	37.0	41.0
23	39.512091226086135	41.0	39.0	41.0	37.0	41.0
24	39.47581350889239	41.0	39.0	41.0	37.0	41.0
25	39.41573030682446	41.0	39.0	41.0	37.0	41.0
26	39.3483231686794	40.0	39.0	41.0	36.0	41.0
27	39.278236870094375	40.0	39.0	41.0	36.0	41.0
28	39.239061889952474	40.0	39.0	41.0	36.0	41.0
29	39.18606567308857	40.0	39.0	41.0	36.0	41.0
30	39.12471693794849	40.0	39.0	41.0	36.0	41.0
31	39.065912732070494	40.0	39.0	41.0	35.0	41.0
32	38.988736755657875	40.0	39.0	41.0	35.0	41.0
33	38.931480808325595	40.0	38.0	41.0	35.0	41.0
34	38.86955315912059	40.0	38.0	41.0	35.0	41.0
35	38.85051900319076	40.0	38.0	41.0	35.0	41.0
36	38.81599956918023	40.0	38.0	41.0	35.0	41.0
37	38.77144472716992	40.0	38.0	41.0	35.0	41.0
38	38.734833124655005	40.0	38.0	41.0	35.0	41.0
39	38.686255503049395	40.0	38.0	41.0	35.0	41.0
40	38.628253698991614	40.0	38.0	41.0	35.0	41.0
41	38.60247452104959	40.0	38.0	41.0	35.0	41.0
42	38.564764328123104	40.0	38.0	41.0	35.0	41.0
43	37.70088991208584	40.0	37.0	41.0	33.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	4.0
18	3.0
19	11.0
20	10.0
21	27.0
22	49.0
23	103.0
24	149.0
25	241.0
26	365.0
27	562.0
28	812.0
29	1213.0
30	1745.0
31	2462.0
32	3306.0
33	5001.0
34	7588.0
35	12035.0
36	19947.0
37	37003.0
38	93138.0
39	185609.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.031086338974376	17.16062845833838	12.117344534647334	27.690940668039904
2	20.038773779231793	19.280800247721366	32.89874389111031	27.781682081936538
3	20.618764893574053	20.129245930772647	28.736486395519474	30.515502780133826
4	16.18616799278377	14.770655788467494	31.835157585793716	37.20801863295502
5	17.35557440392046	33.59371003136906	32.27432448806495	16.77639107664553
6	37.617028151379294	32.10361215450274	14.531281554182318	15.748078139935645
7	31.943401052815812	28.113144041897222	19.299379350269934	20.64407555501703
8	29.01813481966154	31.45926733712993	18.87340630343175	20.649191539776783
9	27.83284192953404	13.418958762470211	17.93771961710893	40.810479690886815
10	18.278067234810237	25.203764287733755	31.238202943037546	25.27996553441846
11	38.3798484052937	19.612262207682054	19.75497125624352	22.252918130780728
12	23.826487337937717	23.14552284017933	27.043633964753553	25.984355857129394
13	33.12842468058753	17.559675269598934	21.800826635432234	27.511073414381304
14	24.666316625604157	18.674690684868803	22.771248165650203	33.88774452387684
15	27.57192670678676	25.143988044751403	19.99623032701913	27.287854921442705
16	27.439449627744793	23.870377101929265	21.54206551153116	27.148107758794783
17	26.168262046124642	24.08336362534836	23.178103585228268	26.570270743298735
18	26.155606715403152	23.033778962532143	23.786367246927043	27.02424707513766
19	26.974972064030588	23.328890504463022	23.60865409211465	26.08748333939174
20	27.4359492171197	23.05181954036916	23.12344332700567	26.388787915505475
21	27.556040227795954	23.05289658979226	22.46805875304603	26.923004429365754
22	27.777104621888338	23.210145805565652	22.387549308668902	26.625200263877108
23	27.213538511248437	22.974002719549794	22.501178022806524	27.31128074639525
24	27.325820913607174	22.987196574982836	22.673775192859164	27.01320731855083
25	27.169379484901114	22.362777171937477	22.98961993618482	27.47822340697659
26	27.123604884419134	22.7758256256984	22.886761716278254	27.21380777360421
27	26.42971579358348	23.131521197678957	23.001198217483203	27.43756479125436
28	26.62735436272332	23.00362157868519	23.19560563835373	27.17341842023776
29	26.276505513146738	23.188604817103545	23.670584433943212	26.86430523580651
30	26.333589132571323	23.700741817790163	22.94492238512595	27.02074666451257
31	26.78594989027559	23.192374490084415	22.899147784643965	27.12252783499603
32	26.0503251342946	23.152792923785288	23.63746516418272	27.159416777737388
33	25.889844770251898	22.812445306083983	23.93580785438292	27.361902069281204
34	26.612006408444067	22.40962882184256	23.82137135317797	27.156993416535403
35	25.863726321741588	22.346890692946673	24.720976883826758	27.06840610148498
36	25.88876772082879	22.31538699732084	24.379013691990792	27.41683158985958
37	25.64239266529343	21.322347429217658	25.25761675888902	27.77764314659989
38	24.864224457099777	21.494944599270298	26.258734197665497	27.38209674596443
39	24.744671971135077	20.992770305747406	27.026670436339646	27.23588728677787
40	24.026010743567998	20.68392638367193	28.166188725985165	27.123874146774913
41	23.544838913795658	20.709506307470686	28.43814370531928	27.307511073414382
42	23.122097015226785	20.656192361026967	28.82238108701213	27.39932953673412
43	22.330196426888538	20.289726294815353	29.41637384385476	27.96370343444135
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	4.0
2	5.0
3	12.0
4	19.0
5	19.0
6	20.5
7	22.0
8	28.0
9	34.0
10	43.5
11	53.0
12	53.0
13	99.0
14	145.0
15	239.5
16	334.0
17	364.5
18	395.0
19	395.0
20	588.0
21	781.0
22	960.0
23	1139.0
24	1508.0
25	1877.0
26	1877.0
27	2309.5
28	2742.0
29	3472.0
30	4202.0
31	5128.5
32	6055.0
33	6055.0
34	7085.0
35	8115.0
36	9587.0
37	11059.0
38	12540.5
39	14022.0
40	14022.0
41	15481.5
42	16941.0
43	18046.0
44	19151.0
45	20511.5
46	21872.0
47	21872.0
48	23291.5
49	24711.0
50	26462.0
51	28213.0
52	29951.5
53	31690.0
54	31690.0
55	31021.5
56	30353.0
57	30351.5
58	30350.0
59	29032.5
60	27715.0
61	27715.0
62	26231.0
63	24747.0
64	22260.0
65	19773.0
66	17312.0
67	14851.0
68	14851.0
69	12722.5
70	10594.0
71	9136.0
72	7678.0
73	6254.5
74	4831.0
75	4831.0
76	3860.5
77	2890.0
78	2393.0
79	1896.0
80	1493.0
81	1090.0
82	1090.0
83	827.5
84	565.0
85	424.0
86	283.0
87	199.5
88	116.0
89	116.0
90	85.0
91	54.0
92	33.0
93	12.0
94	7.5
95	3.0
96	3.0
97	2.5
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	371385.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.6617005730005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.83667815060782	74.99539312753953
2	5.2463523718848615	8.56852112986625
3	1.188518592461927	2.9116934826920966
4	0.51803776435635	1.6921537919349934
5	0.2805214438279075	1.1453929075090181
6	0.1644179154773576	0.8055987949529327
7	0.13177307859052081	0.7532569581209103
8	0.10086256504660111	0.6589286868688262
9	0.07682349360842032	0.5646183418820374
>10	0.431600561721891	6.259375930956668
>50	0.02078494503410745	1.1210486661378158
>100	0.003629117382259071	0.524018181538942
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	380	0.10231969519501326	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	2.692623557763507E-4	0.0
15	0.0	0.0	0.0	2.692623557763507E-4	0.0
16	0.0	0.0	0.0	5.385247115527014E-4	0.0
17	0.0	0.0	0.0	5.385247115527014E-4	0.0
18	0.0	0.0	0.0	5.385247115527014E-4	0.0
19	0.0	0.0	0.0	5.385247115527014E-4	0.0
20	0.0	0.0	0.0	8.077870673290521E-4	0.0
21	0.0	0.0	0.0	8.077870673290521E-4	0.0
22	0.0	0.0	0.0	0.0018848364904344547	0.0
23	0.0	0.0	0.0	0.0024233612019871563	0.0
24	0.0	0.0	0.0	0.004308197692421611	0.0
25	0.0	0.0	0.0	0.006193034182856065	0.0
26	0.0	0.0	0.0	0.009693444807948625	0.0
27	0.0	0.0	0.0	0.03473484389514924	0.0
28	0.0	0.0	0.0	0.1278996189937666	0.0
29	0.0	0.0	0.0	0.24502874375647912	0.0
30	0.0	0.0	0.0	0.38962262880837945	0.0
31	0.0	0.0	0.0	0.8468301089166229	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCGA	30	8.296831E-6	37.0	20
TCGCGGT	35	2.3839879E-5	31.714285	17
GTCATAA	30	3.5948292E-4	30.833332	1
TATAGAG	25	0.0054916115	29.6	7
GTCGAAA	40	5.9294704E-5	27.750002	22
TCTTAAT	35	8.8580465E-4	26.42857	2
TAGTGGG	35	8.8580465E-4	26.42857	7
CGAAATA	45	1.3208567E-4	24.666668	24
GGTCGAA	45	1.3208567E-4	24.666668	21
ATCGCGG	55	1.8981664E-5	23.545454	16
GAATAAG	40	0.0019287394	23.125002	1
GGTATCA	160	0.0	23.125002	1
ACACTAG	40	0.0019287394	23.125002	6
TTATGTT	45	0.0038210049	20.555557	4
TAATACC	55	5.13409E-4	20.181818	4
TCGAAAT	55	5.13409E-4	20.181818	23
CATCGCG	75	9.2381015E-6	19.733334	15
CCTTATC	50	0.0070261345	18.5	2
TACCCAC	50	0.0070261345	18.5	29
AGGGTTG	50	0.0070261345	18.5	6
>>END_MODULE