FastQCFastQC Report
Fri 10 Feb 2017
ERR1632008.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632008.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365980
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34330.9380294005136893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30470.8325591562380459No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27560.7530466145691022No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11450.31285862615443466No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.13743920432810536No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3860.10547024427564348No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3760.10273785452756981No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.10137165965353298No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3710.10137165965353298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5900.030.4152551
AACCGCT250.005491509629.67
AGACGTT250.005491509629.65
ATACACC358.857799E-426.428574
AGGGACG400.001928686323.1258
ACGGAGT705.086258E-621.14285726
ACTGATC806.9334783E-720.81258
ACGCCGT555.1338907E-420.18181824
CGCCGTC555.1338907E-420.18181825
CTAGAAC1355.4569682E-1119.1851863
AGGACGC609.2204247E-418.521
AACGGAG801.6117689E-518.525
CATACAC609.2204247E-418.53
CGGAGTC801.6117689E-518.527
GTCTAGA1156.369555E-817.6956521
TAGACAT1054.775211E-717.6190475
CAACGGA852.7149124E-517.41176424
CGTCTTC752.0624185E-417.26666622
TGTCTCG752.0624185E-417.26666610
ACAACGC650.001577153117.0769238
Produced by FastQC (version 0.11.5)