FastQCFastQC Report
Fri 10 Feb 2017
ERR1632007.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632007.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188191
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31781.6887098745423532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT25221.3401278488344288No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20331.0802854546710523No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT15080.8013135590968749No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT14710.7816526826468854RNA PCR Primer, Index 29 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8310.4415726575659835No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC7350.3905606538038482RNA PCR Primer, Index 29 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC7150.37993315302007No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG6240.33157802445387935No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT5860.31138577296470077No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC4330.23008539196879765No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC3700.1966087644998964No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA3290.1748223878931511No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC3260.17322826277558437RNA PCR Primer, Index 29 (95% over 24bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2820.14984776105127237No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC2820.14984776105127237No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT2790.14825363593370564No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2580.13709476011073857No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC2500.1328437597972273No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG2230.11849663373912675No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCC2180.1158397585431822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCATCCA355.5979945E-737.014
CAATGCG308.275065E-637.019
AATGCGA352.3777844E-531.71428520
ATCCAAT352.3777844E-531.71428516
GTCATCC303.5881484E-430.83333213
ATAATTC250.005484813429.59999815
GTATCAG250.005484813429.59999817
TGAGGCC250.005484813429.59999826
CGAGTCC405.9141654E-527.7527
TCCAATG405.9141654E-527.7517
ATGCGAC405.9141654E-527.7521
ACGAGTC405.9141654E-527.7526
GGTATCA8250.027.581821
ATCGTCA358.8417414E-426.4285710
CATCGTC358.8417414E-426.428579
TATACAC2250.026.3111113
TGTCTCG509.03844E-625.89999810
AGGCATC509.03844E-625.8999986
GTCACTA509.03844E-625.8999981
GTACTAG509.03844E-625.8999981
Produced by FastQC (version 0.11.5)