##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632007.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	188191
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.73669304058111	34.0	31.0	34.0	31.0	34.0
2	32.88101450122482	34.0	31.0	34.0	31.0	34.0
3	32.92543214075062	34.0	31.0	34.0	31.0	34.0
4	36.34003751507777	37.0	37.0	37.0	35.0	37.0
5	36.24019214521417	37.0	35.0	37.0	35.0	37.0
6	36.30277218357945	37.0	37.0	37.0	35.0	37.0
7	36.27491750402517	37.0	37.0	37.0	35.0	37.0
8	36.28803183999235	37.0	37.0	37.0	35.0	37.0
9	38.063855338459334	39.0	38.0	39.0	37.0	39.0
10	38.0068813067575	39.0	38.0	39.0	35.0	39.0
11	38.0959131945736	39.0	38.0	39.0	37.0	39.0
12	38.04656970843452	39.0	38.0	39.0	35.0	39.0
13	38.09336790813588	39.0	38.0	39.0	37.0	39.0
14	39.344692360421064	40.0	39.0	41.0	37.0	41.0
15	39.36678693455054	40.0	39.0	41.0	37.0	41.0
16	39.350611878357626	40.0	39.0	41.0	37.0	41.0
17	39.37723376782099	40.0	39.0	41.0	37.0	41.0
18	39.457152573715	40.0	39.0	41.0	37.0	41.0
19	39.464506804257375	41.0	39.0	41.0	37.0	41.0
20	39.47004904591612	41.0	39.0	41.0	37.0	41.0
21	39.439962591197244	41.0	39.0	41.0	37.0	41.0
22	39.37751539659176	40.0	39.0	41.0	36.0	41.0
23	39.313474076868715	40.0	39.0	41.0	36.0	41.0
24	39.239804241435564	40.0	39.0	41.0	36.0	41.0
25	39.16434898587074	40.0	39.0	41.0	35.0	41.0
26	39.029751688444186	40.0	38.0	41.0	35.0	41.0
27	38.90042563140639	40.0	38.0	41.0	35.0	41.0
28	38.84408393600119	40.0	38.0	41.0	35.0	41.0
29	38.71326471510327	40.0	38.0	41.0	35.0	41.0
30	38.572487525970956	40.0	38.0	41.0	35.0	41.0
31	38.36893368970886	40.0	37.0	41.0	35.0	41.0
32	38.189201396453605	40.0	37.0	41.0	35.0	41.0
33	38.028752703370515	40.0	37.0	41.0	34.0	41.0
34	37.887816101726436	40.0	36.0	41.0	34.0	41.0
35	37.7728637394987	40.0	36.0	41.0	33.0	41.0
36	37.63835677582881	40.0	36.0	41.0	33.0	41.0
37	37.49464108272978	40.0	35.0	41.0	33.0	41.0
38	37.38159635689273	40.0	35.0	41.0	33.0	41.0
39	37.20772513031973	40.0	35.0	41.0	33.0	41.0
40	36.98229989744462	40.0	35.0	41.0	32.0	41.0
41	36.804087336801445	39.0	35.0	41.0	32.0	41.0
42	36.59670228650679	39.0	35.0	41.0	31.0	41.0
43	35.71979000058451	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	0.0
14	0.0
15	0.0
16	1.0
17	3.0
18	8.0
19	4.0
20	10.0
21	23.0
22	63.0
23	88.0
24	140.0
25	251.0
26	429.0
27	622.0
28	929.0
29	1335.0
30	1840.0
31	2423.0
32	3109.0
33	3911.0
34	5564.0
35	8092.0
36	12952.0
37	27782.0
38	45652.0
39	72956.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.287356993692576	19.00675377674809	14.650009830438224	24.055879399121103
2	19.38774967984654	21.210897439303686	34.05688901169556	25.34446386915421
3	19.548756316720777	23.495278732776807	33.09828844099877	23.85767650950364
4	13.641991381096865	17.299977150873314	31.77888421869271	37.27914724933711
5	14.24244517538033	36.69676020638607	31.54879882672391	17.51199579150969
6	30.847915150033746	34.44638691542104	16.286113576100878	18.419584358444347
7	26.610730587541383	32.90221105153807	19.4616108102938	21.025447550626758
8	31.028051288318782	30.12471372169764	18.68102087772529	20.16621411225829
9	27.8700894304191	12.062213389588237	19.658219574793694	40.40947760519897
10	19.530689565388357	27.314802514466685	28.480107975407964	24.674399944736997
11	35.591500124873136	21.095589055799692	19.028540153354836	24.28437066597234
12	22.771014554362324	29.686329314366787	24.95549734046793	22.58715879080296
13	34.00906525816856	17.992890201975655	24.07925990084542	23.91878463901037
14	23.8311077575442	21.599863967989968	26.076167298117337	28.4928609763485
15	27.617154911765173	26.96196948844525	21.80231785792094	23.618557741868635
16	25.6229043896892	24.26364703944397	24.348135670675006	25.765312900191827
17	21.702419350553427	28.99341626326445	25.24243986162994	24.061724524552183
18	23.408664601389013	22.482477908082746	28.108145447975723	26.000712042552514
19	23.752995626783427	25.215339734631304	29.839896700692382	21.191767937892887
20	25.901876285263377	22.29968489460176	29.2160624046846	22.582376415450263
21	26.778645099925075	22.04621900090865	27.70270629307459	23.472429606091683
22	26.56556370921032	25.134570728674593	26.468322077038753	21.83154348507633
23	24.53252280927356	23.04467269954461	27.252631634881585	25.170172856300248
24	22.93733494162845	25.005446594151685	28.907864881955035	23.149353582264826
25	22.602037291900253	24.8035240792599	28.75057787035512	23.84386075848473
26	23.057957075524335	25.35084036962448	27.94129368567041	23.64990886918078
27	22.558995913725948	26.297219314419923	28.51996110334713	22.623823668506997
28	21.853329861683076	24.82371633074908	28.915304132503678	24.407649675064164
29	21.123220557837517	25.90028216014581	30.286251733611063	22.69024554840561
30	21.872990738133065	25.296640115627206	30.572662879733887	22.257706266505835
31	22.09244862931809	25.999117917434944	27.16761162861136	24.74082182463561
32	20.9133274173579	26.211667933110512	29.24263115664405	23.632373492887545
33	21.35649420004145	25.49696850540143	29.99133858686122	23.155198707695902
34	21.665223097810205	25.196210233220505	29.203840778783256	23.934725890186034
35	21.43248083064546	25.467742878246035	31.492473072569886	21.60730321853861
36	21.590830592323755	26.062351547098427	28.060321694448724	24.286496166129094
37	20.18587498870828	25.507064631146015	29.761253194892422	24.54580718525328
38	20.95530604545382	23.580830114086222	30.78149327013513	24.682370570324828
39	21.66734859796696	23.76096625237126	30.65077501049466	23.920910139167123
40	19.98288972373812	23.856082384386077	32.478173770265315	23.682854121610493
41	20.541896264964848	23.232248088378295	31.09713004341334	25.128725603243513
42	19.292102172792536	24.1855349086832	32.82250479565973	23.699858122864537
43	18.46315711165784	23.271569841278275	32.53768777465447	25.727585272409414
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	38.0
1	36.0
2	34.0
3	103.0
4	172.0
5	172.0
6	286.0
7	400.0
8	393.5
9	387.0
10	580.5
11	774.0
12	774.0
13	1572.0
14	2370.0
15	4012.5
16	5655.0
17	4879.5
18	4104.0
19	4104.0
20	4296.5
21	4489.0
22	3123.0
23	1757.0
24	1792.0
25	1827.0
26	1827.0
27	1996.5
28	2166.0
29	2391.5
30	2617.0
31	2987.5
32	3358.0
33	3358.0
34	3770.5
35	4183.0
36	4620.5
37	5058.0
38	6097.0
39	7136.0
40	7136.0
41	7997.5
42	8859.0
43	9603.5
44	10348.0
45	11118.0
46	11888.0
47	11888.0
48	13092.0
49	14296.0
50	14977.0
51	15658.0
52	14366.0
53	13074.0
54	13074.0
55	14081.0
56	15088.0
57	14663.0
58	14238.0
59	13275.5
60	12313.0
61	12313.0
62	10477.5
63	8642.0
64	7347.5
65	6053.0
66	5316.5
67	4580.0
68	4580.0
69	3495.0
70	2410.0
71	2045.0
72	1680.0
73	1340.0
74	1000.0
75	1000.0
76	798.5
77	597.0
78	494.0
79	391.0
80	319.5
81	248.0
82	248.0
83	185.5
84	123.0
85	111.5
86	100.0
87	74.5
88	49.0
89	49.0
90	37.5
91	26.0
92	15.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	188191.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.440499316389165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.95099995905778	42.1918085934027
2	11.35299999411259	12.134199768486804
3	3.794999998031998	6.084200844015778
4	1.8200000505322032	3.8904684582517786
5	1.0339999998426677	2.762873814236925
6	0.5939999996947837	1.9046193946574554
7	0.4619999997604456	1.7282657469958926
8	0.3089999998397598	1.321049142416075
9	0.2569999998667256	1.236078748547079
>10	1.2939999993289608	12.297727696310153
>50	0.08099999995799523	2.960603660592658
>100	0.039999999979256906	3.8979500181160374
>500	0.004999999997407113	1.865607830167684
>1k	0.004999999997407113	5.7245462838029875
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3178	1.6887098745423532	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2522	1.3401278488344288	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2033	1.0802854546710523	No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT	1508	0.8013135590968749	No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT	1471	0.7816526826468854	RNA PCR Primer, Index 29 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	831	0.4415726575659835	No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC	735	0.3905606538038482	RNA PCR Primer, Index 29 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC	715	0.37993315302007	No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG	624	0.33157802445387935	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	586	0.31138577296470077	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	433	0.23008539196879765	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	370	0.1966087644998964	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	329	0.1748223878931511	No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC	326	0.17322826277558437	RNA PCR Primer, Index 29 (95% over 24bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	282	0.14984776105127237	No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTC	282	0.14984776105127237	No Hit
CTCCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCAT	279	0.14825363593370564	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	258	0.13709476011073857	No Hit
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC	250	0.1328437597972273	No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG	223	0.11849663373912675	No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCC	218	0.1158397585431822	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	5.313750391889091E-4	0.0
4	0.0	0.0	0.0	5.313750391889091E-4	0.0
5	0.0	0.0	0.0	5.313750391889091E-4	0.0
6	0.0	0.0	0.0	5.313750391889091E-4	0.0
7	0.0	0.0	0.0	5.313750391889091E-4	0.0
8	0.0	0.0	0.0	5.313750391889091E-4	0.0
9	0.0	0.0	0.0	0.0015941251175667275	0.0
10	0.0	5.313750391889091E-4	0.0	0.0021255001567556365	0.0
11	0.0	5.313750391889091E-4	0.0	0.0021255001567556365	0.0
12	0.0	5.313750391889091E-4	0.0	0.0021255001567556365	0.0
13	0.0	5.313750391889091E-4	0.0	0.0021255001567556365	0.0
14	0.0	5.313750391889091E-4	0.0	0.0021255001567556365	0.0
15	0.0	5.313750391889091E-4	0.0	0.0021255001567556365	0.0
16	0.0	5.313750391889091E-4	0.0	0.004251000313511273	0.0
17	0.0	5.313750391889091E-4	5.313750391889091E-4	0.004782375352700183	0.0
18	0.0	5.313750391889091E-4	5.313750391889091E-4	0.004782375352700183	0.0
19	0.0	5.313750391889091E-4	5.313750391889091E-4	0.004782375352700183	0.0
20	0.0	5.313750391889091E-4	5.313750391889091E-4	0.005845125431078	0.0
21	0.0	5.313750391889091E-4	5.313750391889091E-4	0.006907875509455819	0.0
22	0.0	5.313750391889091E-4	5.313750391889091E-4	0.009564750705400365	0.0
23	0.0	5.313750391889091E-4	5.313750391889091E-4	0.011158875822967092	0.0
24	0.0	5.313750391889091E-4	5.313750391889091E-4	0.021255001567556366	0.0
25	0.0	5.313750391889091E-4	5.313750391889091E-4	0.02603737692025655	0.0
26	0.0	5.313750391889091E-4	5.313750391889091E-4	0.029757002194578913	0.0
27	0.0	5.313750391889091E-4	5.313750391889091E-4	0.06323362966348019	0.0
28	0.0	5.313750391889091E-4	5.313750391889091E-4	0.14400263562019439	0.0
29	0.0	5.313750391889091E-4	5.313750391889091E-4	0.28853664627957765	0.0
30	0.0	5.313750391889091E-4	5.313750391889091E-4	0.49099053621055205	0.0
31	0.0	5.313750391889091E-4	5.313750391889091E-4	1.0510598275156622	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCATCCA	35	5.5979945E-7	37.0	14
CAATGCG	30	8.275065E-6	37.0	19
AATGCGA	35	2.3777844E-5	31.714285	20
ATCCAAT	35	2.3777844E-5	31.714285	16
GTCATCC	30	3.5881484E-4	30.833332	13
ATAATTC	25	0.0054848134	29.599998	15
GTATCAG	25	0.0054848134	29.599998	17
TGAGGCC	25	0.0054848134	29.599998	26
CGAGTCC	40	5.9141654E-5	27.75	27
TCCAATG	40	5.9141654E-5	27.75	17
ATGCGAC	40	5.9141654E-5	27.75	21
ACGAGTC	40	5.9141654E-5	27.75	26
GGTATCA	825	0.0	27.58182	1
ATCGTCA	35	8.8417414E-4	26.42857	10
CATCGTC	35	8.8417414E-4	26.42857	9
TATACAC	225	0.0	26.311111	3
TGTCTCG	50	9.03844E-6	25.899998	10
AGGCATC	50	9.03844E-6	25.899998	6
GTCACTA	50	9.03844E-6	25.899998	1
GTACTAG	50	9.03844E-6	25.899998	1
>>END_MODULE