FastQCFastQC Report
Fri 10 Feb 2017
ERR1632004.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632004.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110207
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT5250.47637627373941765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4610.41830373751213623No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3720.33754661682107306No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT3490.31667679911439384No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC2680.2431787454517408No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC2170.19690219314562593No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGAC2140.19418004300997216No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAA1780.16151424138212636No Hit
TAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACCTGTC1720.15606994111081873No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA1710.15516255773226745No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCT1680.15244040759661365No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGC1630.14790349070385728No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTACTGTC1610.14608872394675476No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG1570.14245919043254968No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGG1490.13520012340413948No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG1410.1279410563757293No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.12703367299717805No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT1320.11977460596876786Illumina PCR Primer Index 10 (95% over 21bp)
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC1310.11886722259021659No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCCTGTCTCTTA1310.11886722259021659No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCC1250.11342292231890896No Hit
GCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACCTGTCTCTTA1170.10616385529049878No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT1130.1025343217762937No Hit
GCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTT1130.1025343217762937No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCTC200.001834724737.05
GGTATCA1500.034.5333331
GCCGTCT303.578583E-430.83333436
CCGTCTT250.00547507329.59999837
AACGCCC250.00547507329.59999812
AACTGAC250.00547507329.59999830
CGTAGTA358.818395E-426.4285722
CTTATAC1250.023.6837
AATACCA400.001920188123.1250025
TGCCGTC450.003804226220.55555735
CGCAGCT1750.020.08571431
GATACCG2150.019.79069726
ATACCGC2150.019.79069727
TAGAGTG759.141762E-619.7333345
TACCGCA1850.019.028
CGCGGGA701.20655335E-418.520
GGATCTT609.164777E-418.56
GGATACC2400.018.525
TGGATAC2400.018.524
GCAGCTG609.164777E-418.532
Produced by FastQC (version 0.11.5)