##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632004.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	110207
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72584318600452	34.0	31.0	34.0	31.0	34.0
2	32.89268376781874	34.0	31.0	34.0	31.0	34.0
3	32.96813269574528	34.0	31.0	34.0	31.0	34.0
4	36.3729799377535	37.0	37.0	37.0	35.0	37.0
5	36.25073724899507	37.0	37.0	37.0	35.0	37.0
6	36.28306731877286	37.0	37.0	37.0	35.0	37.0
7	36.26024662680229	37.0	37.0	37.0	35.0	37.0
8	36.26497409420454	37.0	37.0	37.0	35.0	37.0
9	38.05816327456514	39.0	38.0	39.0	35.0	39.0
10	37.98579037629189	39.0	38.0	39.0	35.0	39.0
11	38.08168265173718	39.0	38.0	39.0	37.0	39.0
12	38.03780159155045	39.0	38.0	39.0	35.0	39.0
13	38.086310306967796	39.0	38.0	39.0	37.0	39.0
14	39.3883873075213	40.0	39.0	41.0	37.0	41.0
15	39.406289981580116	40.0	39.0	41.0	37.0	41.0
16	39.39516546135908	40.0	39.0	41.0	37.0	41.0
17	39.407832533323656	40.0	39.0	41.0	37.0	41.0
18	39.429555291406174	40.0	39.0	41.0	37.0	41.0
19	39.436796210767014	40.0	39.0	41.0	37.0	41.0
20	39.418131334670214	40.0	39.0	41.0	37.0	41.0
21	39.394711769669804	40.0	39.0	41.0	37.0	41.0
22	39.35776311849519	40.0	39.0	41.0	36.0	41.0
23	39.28170624370503	40.0	39.0	41.0	36.0	41.0
24	39.24349632963423	40.0	39.0	41.0	36.0	41.0
25	39.16684965564801	40.0	39.0	41.0	36.0	41.0
26	39.06622083896667	40.0	39.0	41.0	35.0	41.0
27	38.971290389902634	40.0	39.0	41.0	35.0	41.0
28	38.91926102697651	40.0	38.0	41.0	35.0	41.0
29	38.8122896004791	40.0	38.0	41.0	35.0	41.0
30	38.72133349061312	40.0	38.0	41.0	35.0	41.0
31	38.62909797018338	40.0	38.0	41.0	35.0	41.0
32	38.51484025515621	40.0	38.0	41.0	35.0	41.0
33	38.418385402016206	40.0	38.0	41.0	34.0	41.0
34	38.31843712286879	40.0	38.0	41.0	34.0	41.0
35	38.26696126380357	40.0	38.0	41.0	34.0	41.0
36	38.20608491293657	40.0	38.0	41.0	34.0	41.0
37	38.13923797943869	40.0	37.0	41.0	34.0	41.0
38	38.07527652508461	40.0	37.0	41.0	34.0	41.0
39	38.02457194189117	40.0	37.0	41.0	34.0	41.0
40	37.92952353298792	40.0	37.0	41.0	33.0	41.0
41	37.858375602275714	40.0	37.0	41.0	33.0	41.0
42	37.7435190142187	40.0	37.0	41.0	33.0	41.0
43	36.90726541871206	39.0	35.0	41.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	0.0
16	1.0
17	1.0
18	1.0
19	8.0
20	7.0
21	17.0
22	33.0
23	69.0
24	85.0
25	130.0
26	193.0
27	272.0
28	414.0
29	598.0
30	802.0
31	1077.0
32	1483.0
33	1999.0
34	2960.0
35	4544.0
36	6862.0
37	13341.0
38	27148.0
39	48161.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.71688731205822	18.925295126443874	13.236908726305952	25.12090883519196
2	19.549574890887147	20.78633843585253	33.14490005172085	26.519186621539465
3	20.47238378687379	21.69735134791801	31.762047782808718	26.068217082399485
4	14.82664440552778	15.688658615151487	33.66755287776638	35.81714410155435
5	15.522607456876605	35.168364985890186	32.59139619080458	16.717631366428627
6	33.48607620205613	35.00322121099386	15.111562786392879	16.399139800557133
7	28.495467620024133	31.84552705363543	19.67842333064143	19.980581995699005
8	29.96996561016995	30.273031658606076	19.14215975391763	20.614842977306342
9	28.006387978985003	12.638035696462113	19.123104702968053	40.232471621584835
10	17.832805538668143	27.120781801518962	31.015271262261017	24.03114139755188
11	37.22994002195868	20.17839157222318	19.77369858538931	22.81796982042883
12	23.720816282087345	26.757828450098454	26.557296723438622	22.964058544375586
13	33.66573811100928	17.81647263785422	22.46227553603673	26.055513715099764
14	24.264338925839557	20.310869545491666	24.531109639133632	30.893681889535145
15	27.418403549683777	27.03730253069224	20.295444028056295	25.248849891567687
16	27.001914578928744	24.432204850871543	22.48133058698631	26.084549983213407
17	24.312430244902774	26.12447485186966	23.857831172248588	25.705263730978977
18	25.20801763953288	22.381518415345667	25.575507907846145	26.834956037275308
19	25.444844701334763	24.007549429709545	26.139900369305035	24.40770549965066
20	28.015461812770514	22.666436796210768	24.764307167421308	24.55379422359741
21	28.645185877485098	22.48949703739327	24.24528387488998	24.620033210231654
22	28.316713094449536	22.96859546126834	23.442249584872105	25.272441859410023
23	26.003792862522346	22.541217889970692	24.31878192855263	27.13620731895433
24	25.033800030851033	24.000290362681138	25.768780567477567	25.197129038990262
25	25.73611476584972	23.337900496338708	24.65360639523805	26.27237834257352
26	25.741559066121027	24.262524159082453	24.611866759824693	25.384050014971827
27	24.483925703448964	25.32234794523034	25.14268603627719	25.05104031504351
28	24.97391272786665	23.024853230738522	26.32228442839384	25.678949613000988
29	23.452230802036166	24.943061692995908	26.711551897792337	24.893155607175586
30	23.930421842532688	24.272505376246517	25.92394312520983	25.873129656010963
31	25.085520883428458	25.2969412106309	23.986679612002867	25.63085829393777
32	23.484896603664012	24.11734281851425	25.57278575771049	26.824974820111247
33	23.33336357944595	23.685428330323845	26.440244267605507	26.540963822624697
34	23.734427032765616	23.745315633308227	27.029136080285284	25.491121253640873
35	24.568312357654232	23.7589263839865	27.377571297648966	24.2951899607103
36	23.797036485885652	24.237117424483017	25.784206084912935	26.181640004718393
37	22.865153756113497	23.585616158683205	27.006451495821498	26.5427785893818
38	23.205422523070222	22.9041712413912	28.262270091736458	25.628136143802116
39	22.877857123413214	22.715435498652536	29.02084259620532	25.385864781728927
40	22.785304018800982	22.31890896222563	29.899189706642954	24.996597312330433
41	23.01940893046721	21.833458854700698	29.917337374213982	25.22979484061811
42	20.946945293856107	22.79074831907229	30.756666999373905	25.5056393876977
43	20.473291170252345	21.56033645775677	30.898218806427902	27.068153565562987
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	6.0
2	7.0
3	18.5
4	30.0
5	30.0
6	38.0
7	46.0
8	56.5
9	67.0
10	99.0
11	131.0
12	131.0
13	252.5
14	374.0
15	634.0
16	894.0
17	846.0
18	798.0
19	798.0
20	866.0
21	934.0
22	828.5
23	723.0
24	889.5
25	1056.0
26	1056.0
27	1228.5
28	1401.0
29	1642.0
30	1883.0
31	2297.0
32	2711.0
33	2711.0
34	3078.0
35	3445.0
36	3906.5
37	4368.0
38	4695.0
39	5022.0
40	5022.0
41	5310.0
42	5598.0
43	5780.5
44	5963.0
45	6306.5
46	6650.0
47	6650.0
48	7134.5
49	7619.0
50	7483.0
51	7347.0
52	7480.5
53	7614.0
54	7614.0
55	7798.0
56	7982.0
57	8089.0
58	8196.0
59	8252.0
60	8308.0
61	8308.0
62	7315.5
63	6323.0
64	5213.5
65	4104.0
66	3538.0
67	2972.0
68	2972.0
69	2489.0
70	2006.0
71	1917.5
72	1829.0
73	1353.0
74	877.0
75	877.0
76	738.5
77	600.0
78	713.0
79	826.0
80	817.0
81	808.0
82	808.0
83	594.0
84	380.0
85	256.0
86	132.0
87	124.5
88	117.0
89	117.0
90	81.5
91	46.0
92	27.5
93	9.0
94	6.5
95	4.0
96	4.0
97	2.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	110207.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.4575389948007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.73954536417259	61.433484261435304
2	5.256809596394724	7.197364958668687
3	1.6541851680031812	3.397243369295961
4	0.876134932732454	2.3991216528895625
5	0.5354894293856451	1.832914424673569
6	0.35522566107760617	1.459072472710445
7	0.25449002584664326	1.2195232607729092
8	0.19882033269269006	1.088860054261526
9	0.16700907946185964	1.028972751277142
>10	0.8496255550400955	10.798769588138684
>50	0.07952813307707601	3.631348280962189
>100	0.03181125323083041	4.036948651174608
>500	0.0013254688846179336	0.47637627373941765
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	525	0.47637627373941765	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	461	0.41830373751213623	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.33754661682107306	No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT	349	0.31667679911439384	No Hit
CATCCAAGGAAGGCAGCAGGCGCGCAAATTACCCACTCCCGAC	268	0.2431787454517408	No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATC	217	0.19690219314562593	No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGAC	214	0.19418004300997216	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAA	178	0.16151424138212636	No Hit
TAGCTGCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACCTGTC	172	0.15606994111081873	No Hit
ATTCCGGAGAGGGAGCCTGAGAAACGGCTACCACATCCAAGGA	171	0.15516255773226745	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCCCCCTCGATGCTCTTAGCT	168	0.15244040759661365	No Hit
AAATTAGAGTGTTCAAAGCAGGCCCGAGCCGCCTGGATACCGC	163	0.14790349070385728	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTACTGTC	161	0.14608872394675476	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	157	0.14245919043254968	No Hit
CAGCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGG	149	0.13520012340413948	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	141	0.1279410563757293	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	140	0.12703367299717805	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	132	0.11977460596876786	Illumina PCR Primer Index 10 (95% over 21bp)
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGC	131	0.11886722259021659	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCCTGTCTCTTA	131	0.11886722259021659	No Hit
CGGCTACCACATCCAAGGAAGGCAGCAGGCGCGCAAATTACCC	125	0.11342292231890896	No Hit
GCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACCTGTCTCTTA	117	0.10616385529049878	No Hit
GGTGAAGAGAGAGCAAGCCCTCTTTGGGAACTTTTGATGTGCT	113	0.1025343217762937	No Hit
GCGGTATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTAATTT	113	0.1025343217762937	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	9.073833785512717E-4	0.0
20	0.0	0.0	0.0	0.0018147667571025434	0.0
21	0.0	0.0	0.0	0.0018147667571025434	0.0
22	0.0	0.0	0.0	0.002722150135653815	0.0
23	0.0	9.073833785512717E-4	0.0	0.00544430027130763	0.0
24	0.0	9.073833785512717E-4	0.0	0.008166450406961446	0.0
25	0.0	9.073833785512717E-4	0.0	0.009073833785512717	0.0
26	0.0	9.073833785512717E-4	0.0	0.012703367299717805	0.0
27	0.0	9.073833785512717E-4	0.0	0.03175841824929451	0.0
28	0.0	9.073833785512717E-4	0.0	0.11433030569746024	0.0
29	0.0	9.073833785512717E-4	0.0	0.3330096999283167	0.0
30	0.0	9.073833785512717E-4	0.0	0.514486375638571	0.0
31	0.0	9.073833785512717E-4	0.0	1.0634533196620903	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAGCTC	20	0.0018347247	37.0	5
GGTATCA	150	0.0	34.533333	1
GCCGTCT	30	3.578583E-4	30.833334	36
CCGTCTT	25	0.005475073	29.599998	37
AACGCCC	25	0.005475073	29.599998	12
AACTGAC	25	0.005475073	29.599998	30
CGTAGTA	35	8.818395E-4	26.428572	2
CTTATAC	125	0.0	23.68	37
AATACCA	40	0.0019201881	23.125002	5
TGCCGTC	45	0.0038042262	20.555557	35
CGCAGCT	175	0.0	20.085714	31
GATACCG	215	0.0	19.790697	26
ATACCGC	215	0.0	19.790697	27
TAGAGTG	75	9.141762E-6	19.733334	5
TACCGCA	185	0.0	19.0	28
CGCGGGA	70	1.20655335E-4	18.5	20
GGATCTT	60	9.164777E-4	18.5	6
GGATACC	240	0.0	18.5	25
TGGATAC	240	0.0	18.5	24
GCAGCTG	60	9.164777E-4	18.5	32
>>END_MODULE