FastQCFastQC Report
Fri 10 Feb 2017
ERR1632003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1071279
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.18342560621462756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15180.141699781289468No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14450.13488549668200348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6200.029.2419361
GTATCGA358.8689994E-426.42857216
CAAGACG2000.021.2750024
TCTATAC909.476753E-820.5555573
TATACAG1001.2894816E-820.355
GACGGAC1551.8189894E-1219.0967757
CTTATAC11200.018.6651837
ACCGTCG609.2362566E-418.58
AACACGC609.2362566E-418.536
AAGACGG1950.018.0256425
GCGCAAG1653.6379788E-1217.9393961
TATACTT1351.1514203E-917.8148165
TCGCACG1258.576535E-917.7622
TCTTATA19850.016.96221737
GTATCAA11050.016.4072382
TCTAATA803.3822976E-416.18752
CGTCGTA803.3822976E-416.187510
ATACTTC1403.471723E-815.8571436
TAGATAA1659.731593E-1015.6969714
TAATTCG957.058961E-515.5789485
Produced by FastQC (version 0.11.5)