##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632003.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1071279
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.338635406836126	33.0	31.0	34.0	31.0	34.0
2	32.5272183996886	34.0	31.0	34.0	31.0	34.0
3	32.61848687410096	34.0	31.0	34.0	31.0	34.0
4	36.15160009670684	37.0	35.0	37.0	35.0	37.0
5	35.930779003415545	37.0	35.0	37.0	35.0	37.0
6	35.94512260578244	37.0	35.0	37.0	35.0	37.0
7	35.9190416315451	37.0	35.0	37.0	35.0	37.0
8	35.8984176857756	37.0	35.0	37.0	35.0	37.0
9	37.62433128998141	39.0	37.0	39.0	35.0	39.0
10	37.53206307600541	39.0	37.0	39.0	35.0	39.0
11	37.62227113571721	39.0	37.0	39.0	35.0	39.0
12	37.57624671070748	39.0	37.0	39.0	35.0	39.0
13	37.61038627659088	39.0	37.0	39.0	35.0	39.0
14	38.89246592157598	40.0	38.0	41.0	36.0	41.0
15	38.883012735244506	40.0	38.0	41.0	36.0	41.0
16	38.874279249383214	40.0	38.0	41.0	36.0	41.0
17	38.86302074436258	40.0	38.0	41.0	36.0	41.0
18	38.86889876493425	40.0	38.0	41.0	36.0	41.0
19	38.90380003715185	40.0	38.0	41.0	36.0	41.0
20	38.88383791710656	40.0	38.0	41.0	36.0	41.0
21	38.855912418706986	40.0	38.0	41.0	35.0	41.0
22	38.80895639697969	40.0	38.0	41.0	35.0	41.0
23	38.770262461973026	40.0	38.0	41.0	35.0	41.0
24	38.7202344113905	40.0	38.0	41.0	35.0	41.0
25	38.661935872914526	40.0	38.0	41.0	35.0	41.0
26	38.57142723790908	40.0	38.0	41.0	35.0	41.0
27	38.486452175390355	40.0	38.0	41.0	34.0	41.0
28	38.42623630258784	40.0	38.0	41.0	34.0	41.0
29	38.34730355024228	40.0	38.0	41.0	34.0	41.0
30	38.28259211652613	40.0	38.0	41.0	34.0	41.0
31	38.19343793726937	40.0	38.0	41.0	34.0	41.0
32	38.114953247473345	40.0	38.0	41.0	34.0	41.0
33	38.03930255330311	40.0	38.0	41.0	33.0	41.0
34	37.97435868713939	40.0	37.0	41.0	33.0	41.0
35	37.94698579921757	40.0	37.0	41.0	33.0	41.0
36	37.921739341478734	40.0	37.0	41.0	33.0	41.0
37	37.87275023593293	40.0	37.0	41.0	33.0	41.0
38	37.829826777151425	40.0	37.0	41.0	33.0	41.0
39	37.783397228919824	40.0	37.0	41.0	33.0	41.0
40	37.724149357917035	40.0	37.0	41.0	33.0	41.0
41	37.68389373823253	40.0	37.0	41.0	33.0	41.0
42	37.65103021715165	40.0	37.0	41.0	33.0	41.0
43	36.78816162736318	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	6.0
17	11.0
18	26.0
19	39.0
20	86.0
21	158.0
22	348.0
23	588.0
24	874.0
25	1515.0
26	2418.0
27	3564.0
28	5309.0
29	7215.0
30	10347.0
31	13978.0
32	18943.0
33	26000.0
34	36652.0
35	53293.0
36	85338.0
37	156975.0
38	334109.0
39	313484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.157831899999906	18.831882264097402	12.725629831257775	26.284656004644912
2	19.30617514204983	20.6626845107577	33.55157713350117	26.4795632136913
3	20.12416933403903	21.64384814786811	30.093094329301707	28.138888188791157
4	15.268104760757936	15.533955206813538	34.60359066125631	34.594349371172214
5	15.862534409803608	34.91172701042399	33.774114866435355	15.451623713337048
6	35.255988402647674	34.65465112263006	15.204255847449637	14.885104627272632
7	29.798959934806902	29.962969497208476	20.7028234474866	19.535247120498024
8	28.029112864155834	31.929404011466666	20.06592120259988	19.97556192177761
9	27.085847851026667	13.939879340489266	19.591815017376426	39.38245779110764
10	17.35803651523086	26.206805136663746	32.913368039511646	23.521790308593747
11	36.45604926447732	21.050351962467293	21.084236692775647	21.409362080279738
12	22.981501550949844	25.02784055320789	28.10799054214635	23.88266735369591
13	31.880397170111614	19.07551627540538	23.339578205117434	25.704508349365575
14	23.52375058224795	20.34054620691715	24.408767463937966	31.726935746896935
15	26.069585980869597	27.040014786064138	21.55012839792435	25.340270835141922
16	25.48766474466502	25.745300710645875	23.327256485005307	25.4397780596838
17	24.350799371592274	25.46358138262768	24.973232929983695	25.212386315796355
18	24.34463851153621	24.343891740620325	26.15229085980403	25.159178888039435
19	25.410560647599738	24.546173312461086	25.960744119879138	24.08252192006004
20	26.22183390134596	24.275562201816708	24.930947026871618	24.571656869965715
21	26.296977724757042	24.58155158460121	24.42426296044261	24.697207730199136
22	26.42616909320541	24.63643924691887	24.596860388376886	24.340531271498836
23	25.867491101757807	24.398032632022097	24.396352397461353	25.338123868758743
24	25.39777219566518	24.41362147489123	24.829759567768996	25.3588467616746
25	25.398985698403497	24.26744106810644	24.707662523021547	25.625910710468514
26	24.967445455385572	24.49772654929295	24.91041082668474	25.624417168636743
27	24.95745739438559	24.260253398041034	25.10839846575915	25.673890741814226
28	24.93374741780619	24.679378574582344	24.909010631217452	25.477863376394012
29	24.598260583844173	25.044829591544314	25.492425409253798	24.86448441535772
30	25.138455995123586	25.07983447822649	24.586872327376902	25.194837199273017
31	24.74733472792802	25.2160268240113	24.957550740750076	25.07908770731061
32	24.21815418765793	24.709062718488834	25.37238198452504	25.700401109328197
33	23.994122912891974	24.677978379115057	25.934700484187594	25.393198223805374
34	25.115306096731103	23.528324554107755	25.787399921028975	25.568969428132167
35	24.28639038009706	23.747315125191477	26.350745230700873	25.61554926401059
36	24.074307439985287	24.108752248480556	26.249464425233764	25.567475886300393
37	24.137502928742187	22.788834654651122	27.135414770568637	25.938247646038054
38	23.64351396788325	22.995596851987205	27.501425865717522	25.859463314412025
39	23.27806295092128	22.440746061483516	28.63754446787438	25.643646519720818
40	22.59103370830568	22.122434958586883	29.545617901592397	25.74091343151504
41	22.034969415063678	21.922580392222756	30.13248649511472	25.909963697598855
42	21.63087300320458	21.99987118201701	30.545450811599967	25.823805003178446
43	21.1602206334671	21.66485107987742	30.722155479571615	26.452772807083868
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	64.0
1	67.0
2	70.0
3	160.5
4	251.0
5	251.0
6	335.0
7	419.0
8	428.0
9	437.0
10	619.5
11	802.0
12	802.0
13	1365.0
14	1928.0
15	3021.5
16	4115.0
17	4173.0
18	4231.0
19	4231.0
20	5280.0
21	6329.0
22	7171.0
23	8013.0
24	9928.5
25	11844.0
26	11844.0
27	13738.0
28	15632.0
29	18602.5
30	21573.0
31	25224.5
32	28876.0
33	28876.0
34	33104.0
35	37332.0
36	42064.0
37	46796.0
38	49410.5
39	52025.0
40	52025.0
41	55085.0
42	58145.0
43	60621.5
44	63098.0
45	64821.0
46	66544.0
47	66544.0
48	68274.5
49	70005.0
50	72448.5
51	74892.0
52	76878.5
53	78865.0
54	78865.0
55	77985.0
56	77105.0
57	75867.0
58	74629.0
59	71229.5
60	67830.0
61	67830.0
62	62479.0
63	57128.0
64	51587.5
65	46047.0
66	39889.5
67	33732.0
68	33732.0
69	29458.5
70	25185.0
71	20980.5
72	16776.0
73	12714.0
74	8652.0
75	8652.0
76	6733.0
77	4814.0
78	3907.5
79	3001.0
80	2435.0
81	1869.0
82	1869.0
83	1480.0
84	1091.0
85	849.0
86	607.0
87	466.0
88	325.0
89	325.0
90	243.5
91	162.0
92	94.5
93	27.0
94	18.5
95	10.0
96	10.0
97	5.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1071279.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	72.73547688676565
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.7423244699099	66.0018624413252
2	5.776491919823493	8.403117890418205
3	1.2478271371413538	2.7228390567667167
4	0.5740452980736804	1.6701383403997874
5	0.32306938053365564	1.1749302730313704
6	0.22294611947065845	0.9729655391851302
7	0.15860602118418	0.807539921156065
8	0.11763426235012646	0.684494733620748
9	0.10126872630923159	0.6629246191635579
>10	0.6414096282232093	8.864522391816458
>50	0.057569728978544406	2.904277515238294
>100	0.0357777340003745	4.390695320987833
>500	6.434837510297808E-4	0.27839077193021344
>1k	3.8609025061786854E-4	0.4613011849603537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1965	0.18342560621462756	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1518	0.141699781289468	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1445	0.13488549668200348	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.334636448581555E-5	0.0
3	0.0	0.0	0.0	1.866927289716311E-4	0.0
4	0.0	0.0	0.0	1.866927289716311E-4	0.0
5	0.0	0.0	0.0	1.866927289716311E-4	0.0
6	9.334636448581555E-5	0.0	0.0	1.866927289716311E-4	0.0
7	9.334636448581555E-5	0.0	0.0	1.866927289716311E-4	0.0
8	9.334636448581555E-5	0.0	0.0	1.866927289716311E-4	0.0
9	9.334636448581555E-5	0.0	0.0	1.866927289716311E-4	0.0
10	9.334636448581555E-5	0.0	0.0	2.8003909345744664E-4	0.0
11	9.334636448581555E-5	0.0	0.0	2.8003909345744664E-4	0.0
12	9.334636448581555E-5	0.0	0.0	2.8003909345744664E-4	0.0
13	9.334636448581555E-5	0.0	0.0	2.8003909345744664E-4	0.0
14	9.334636448581555E-5	0.0	0.0	2.8003909345744664E-4	0.0
15	9.334636448581555E-5	0.0	0.0	2.8003909345744664E-4	0.0
16	9.334636448581555E-5	0.0	0.0	7.467709158865244E-4	0.0
17	9.334636448581555E-5	0.0	0.0	8.4011728037234E-4	0.0
18	9.334636448581555E-5	0.0	0.0	9.334636448581555E-4	0.0
19	9.334636448581555E-5	9.334636448581555E-5	0.0	9.334636448581555E-4	0.0
20	9.334636448581555E-5	9.334636448581555E-5	0.0	0.0011201563738297865	0.0
21	9.334636448581555E-5	9.334636448581555E-5	0.0	0.0013068491028014177	0.0
22	9.334636448581555E-5	9.334636448581555E-5	0.0	0.0019602736542021267	0.0
23	1.866927289716311E-4	1.866927289716311E-4	0.0	0.0032671227570035444	0.0
24	1.866927289716311E-4	1.866927289716311E-4	0.0	0.004947357317748225	0.0
25	1.866927289716311E-4	1.866927289716311E-4	0.0	0.007467709158865244	0.0
26	1.866927289716311E-4	1.866927289716311E-4	0.0	0.012135027383156022	0.0
27	1.866927289716311E-4	1.866927289716311E-4	0.0	0.03528492577563828	0.0
28	1.866927289716311E-4	2.8003909345744664E-4	0.0	0.11686964833624107	0.0
29	1.866927289716311E-4	2.8003909345744664E-4	0.0	0.2478345977098403	0.0
30	2.8003909345744664E-4	2.8003909345744664E-4	0.0	0.43191362847586856	0.0
31	2.8003909345744664E-4	2.8003909345744664E-4	0.0	0.9765896652506023	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	620	0.0	29.241936	1
GTATCGA	35	8.8689994E-4	26.428572	16
CAAGACG	200	0.0	21.275002	4
TCTATAC	90	9.476753E-8	20.555557	3
TATACAG	100	1.2894816E-8	20.35	5
GACGGAC	155	1.8189894E-12	19.096775	7
CTTATAC	1120	0.0	18.66518	37
ACCGTCG	60	9.2362566E-4	18.5	8
AACACGC	60	9.2362566E-4	18.5	36
AAGACGG	195	0.0	18.025642	5
GCGCAAG	165	3.6379788E-12	17.939396	1
TATACTT	135	1.1514203E-9	17.814816	5
TCGCACG	125	8.576535E-9	17.76	22
TCTTATA	1985	0.0	16.962217	37
GTATCAA	1105	0.0	16.407238	2
TCTAATA	80	3.3822976E-4	16.1875	2
CGTCGTA	80	3.3822976E-4	16.1875	10
ATACTTC	140	3.471723E-8	15.857143	6
TAGATAA	165	9.731593E-10	15.696971	4
TAATTCG	95	7.058961E-5	15.578948	5
>>END_MODULE