FastQCFastQC Report
Fri 10 Feb 2017
ERR1632002.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632002.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148309
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT37982.560869535901395No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36972.492768476626503No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22441.513057198147112No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15571.0498351414951217No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT8310.5603166362122326No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7700.5191862934818521No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT5940.40051514068600014RNA PCR Primer, Index 41 (95% over 22bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4360.2939808103351786No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3920.2643130221362156No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3160.2130686607016432No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC3000.20228037408383848No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC2800.1887950158115826No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2560.1726125858848755No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG2400.1618242992670708No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2400.1618242992670708No Hit
GTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTTCCGACCTG2310.15575588804455562No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2150.1449676014267509No Hit
GTCTCAGGAGGAGACAGTGTGGCCATCGTTACGCCATTCGTGC1980.1335050468953334No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1950.13148224315449503No Hit
GGTCGGAACTTACCCGACAAGGAATTTCGCTACCTTAGGACCG1890.12743663567281824No Hit
GTGCTGGAAGATTAAATGATGGGGTGCAAGCTCTTGATTGAAG1810.12204249236391587No Hit
CCCTATACGTCCACTTTCGTGTTTGCAGAGTGCTGTGTTTTTA1720.11597408114140072No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTGAT200.001836772437.07
TATAGAC200.001836772437.03
ATAGACA303.5845122E-430.8333344
AAACCCT250.0054811129.637
CAGTACT250.0054811129.64
ACACAGG358.8328664E-426.4285726
TAGACAG502.686755E-422.25
GGACCGT705.052658E-621.1428596
TCGCTAC806.8763984E-720.812527
TGATACA555.1141216E-420.1818185
GAGCTGA759.17686E-619.7333346
GACCGTT759.17686E-619.7333347
GTAAGTT759.17686E-619.73333429
CGCTACC851.2304117E-619.58823428
TACCTTA851.2304117E-619.58823431
TTCGCTA851.2304117E-619.58823426
TTACCCG851.2304117E-619.58823410
ACCTTAG851.2304117E-619.58823432
AAGGAAT951.6516788E-719.47368419
GGTATCA18150.018.5509641
Produced by FastQC (version 0.11.5)