FastQCFastQC Report
Fri 10 Feb 2017
ERR1632001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1417880
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25450.17949332806725535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT23750.16750359691934436No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT17800.125539537901656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10050.025.0348241
GTACGGA1005.8797395E-616.656
GTATCAA15300.016.2026142
CTTATAC14750.015.92881437
TCTTATA24750.015.24848637
GTGCTAC1353.973746E-715.0740731
TATACTG1353.973746E-715.0740735
ACCGTCG1651.6456397E-814.5757578
TACGGAT908.275692E-414.3888897
ATACTGC1458.913412E-714.0344836
GTATTAT1203.3014287E-513.8749991
CATACTG1203.3014287E-513.8749995
AGCGTCA1907.1268005E-913.6315793
GCGGTAT2408.0035534E-1113.10416624
GTTACAC850.009406147513.0588233
CTCTTAT36800.012.76902237
TTATACA8150.012.71165737
TATAGTG2052.0656444E-812.6341465
TAGACGT1050.002644094412.3333334
ACCGACC1050.002644094412.3333338
Produced by FastQC (version 0.11.5)