##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1632001.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1417880
Sequences flagged as poor quality	0
Sequence length	43
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.261322537873443	31.0	31.0	34.0	30.0	34.0
2	31.455076593223687	31.0	31.0	34.0	30.0	34.0
3	31.550604423505515	31.0	31.0	34.0	30.0	34.0
4	35.367363951815385	37.0	35.0	37.0	33.0	37.0
5	34.40861567974723	35.0	35.0	37.0	30.0	37.0
6	34.34360594690665	35.0	35.0	37.0	30.0	37.0
7	34.330916579682345	35.0	35.0	37.0	30.0	37.0
8	34.268166558523994	35.0	35.0	37.0	30.0	37.0
9	35.82414661325359	37.0	35.0	39.0	30.0	39.0
10	35.53350001410556	37.0	35.0	39.0	30.0	39.0
11	35.70803664626062	37.0	35.0	39.0	30.0	39.0
12	35.61991635399328	37.0	35.0	39.0	30.0	39.0
13	35.739255790334866	37.0	35.0	39.0	30.0	39.0
14	36.68580274776427	38.0	36.0	40.0	31.0	41.0
15	36.61049665698084	38.0	36.0	40.0	31.0	41.0
16	36.58155979349451	38.0	36.0	40.0	31.0	41.0
17	36.53682469602505	38.0	36.0	40.0	31.0	41.0
18	36.529463706378536	38.0	35.0	40.0	31.0	41.0
19	36.563577312607556	38.0	35.0	40.0	31.0	41.0
20	36.515531638784665	38.0	35.0	40.0	30.0	41.0
21	36.48080796682371	38.0	35.0	40.0	30.0	41.0
22	36.426289248737554	38.0	35.0	40.0	30.0	41.0
23	36.33920924196688	38.0	35.0	40.0	30.0	41.0
24	36.253364177504444	38.0	35.0	40.0	30.0	41.0
25	36.154903094761195	38.0	35.0	40.0	30.0	41.0
26	35.9680925043022	38.0	34.0	40.0	29.0	41.0
27	35.83904561740063	38.0	34.0	40.0	29.0	41.0
28	35.72205546308573	38.0	34.0	40.0	29.0	41.0
29	35.59465257990803	38.0	34.0	40.0	28.0	41.0
30	35.476245521482774	38.0	34.0	40.0	27.0	41.0
31	35.3588082207239	38.0	34.0	40.0	27.0	41.0
32	35.224699551443	38.0	34.0	40.0	27.0	41.0
33	35.0870383953508	38.0	33.0	40.0	27.0	41.0
34	34.99740246001072	38.0	33.0	40.0	26.0	41.0
35	34.95194374700257	38.0	33.0	40.0	26.0	41.0
36	34.89113817812509	37.0	33.0	40.0	26.0	40.0
37	34.81881259344937	37.0	33.0	40.0	26.0	40.0
38	34.74986176545265	37.0	33.0	39.0	26.0	40.0
39	34.68717592461986	37.0	33.0	39.0	26.0	40.0
40	34.57915691031681	37.0	33.0	39.0	25.0	40.0
41	34.51732727734364	37.0	33.0	39.0	25.0	40.0
42	34.449286258357546	37.0	33.0	39.0	25.0	40.0
43	33.49560823200835	36.0	31.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	2.0
13	4.0
14	19.0
15	26.0
16	62.0
17	125.0
18	249.0
19	468.0
20	814.0
21	1512.0
22	2530.0
23	3953.0
24	6063.0
25	9073.0
26	13227.0
27	18582.0
28	25901.0
29	34758.0
30	46385.0
31	61264.0
32	79324.0
33	102518.0
34	131659.0
35	166704.0
36	202239.0
37	226466.0
38	203982.0
39	79969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.6115609219398	19.448190255874966	12.793889468784384	26.14635935340085
2	20.069822551979012	20.729046181623268	33.20809941602956	25.993031850368155
3	20.921375574801818	22.125708804694334	28.81978728806387	28.133128332439984
4	16.34376675035969	15.977374672045588	32.79219680085762	34.8866617767371
5	16.730964538606933	34.29958811747115	32.496826247637316	16.47262109628459
6	35.66218579851609	33.54712669619432	15.16080345304257	15.629884052247018
7	30.319491071176685	29.002736479814935	20.72756509718735	19.950207351821028
8	27.557198070358563	32.476725816007	19.49678393093915	20.46929218269529
9	26.90855361525658	14.224687561711852	19.6475724320817	39.219186390949865
10	18.155344598978758	25.773196603379695	31.82208649533106	24.249372302310494
11	37.3016757412475	20.944931870115948	20.613168956470222	21.140223432166334
12	22.632944960081247	24.60398623296753	28.075930262081418	24.687138544869804
13	31.3185177871188	18.885166586735124	23.509535362654102	26.28678026349197
14	23.656585888791717	20.094718876068494	24.018111546816375	32.230583688323414
15	26.24460462098344	26.64985753378283	21.125342059976866	25.98019578525686
16	26.172313594944562	25.016785623607074	23.179112477783733	25.631788303664628
17	24.763167545913618	25.39128840240359	24.536984794199793	25.308559257483005
18	25.08294072841143	24.230541371625243	25.347843258949982	25.33867464101334
19	25.630871441870962	24.524712951730756	25.085409202471293	24.75900640392699
20	25.825246142127682	24.177716026744154	24.810844359184134	25.18619347194403
21	26.514514627472003	24.249513357971058	24.29253533444297	24.943436680113972
22	26.417256749513356	24.18921206308009	24.27892346319858	25.11460772420797
23	25.646528620193525	24.172003272491324	24.566818066409006	25.614650040906138
24	25.569300646034925	24.51392219369763	24.268273760826023	25.64850339944142
25	25.70513724715773	23.916410415549976	24.534375264479362	25.844077072812933
26	25.450390724179762	24.568510734335767	24.681284734956414	25.299813806528054
27	25.649631844725928	24.14717747623212	24.45058820210455	25.752602476937398
28	25.205729680932098	24.271447513188704	24.968897226845712	25.55392557903349
29	25.026518464185965	24.36369791519734	25.204812819138432	25.404970801478267
30	25.04520833921065	24.58169943859847	24.898863091375855	25.47422913081502
31	25.620856489970944	24.345431207154345	24.414619008660818	25.619093294213897
32	24.75900640392699	24.28985527689226	24.746522977967107	26.204615341213643
33	24.653214658504247	23.966132535898666	25.396225350523316	25.98442745507377
34	25.56492792055745	23.374968262476372	25.200299038000395	25.859804778965778
35	25.213981437075073	23.500296216887186	25.697520241487293	25.588202104550454
36	24.665980195785256	23.489575986684347	25.726859818884535	26.117583998645866
37	24.70392416847688	22.646274720004513	26.475724320816994	26.174076790701612
38	24.032499224193867	22.524543684938074	27.28940389877846	26.153553192089596
39	23.922405281123932	22.01624961209693	27.81314356645132	26.248201540327816
40	23.653905831241005	22.168166558523993	28.42737044037577	25.750557169859228
41	22.925071233108586	22.258019014303045	28.9768527661015	25.84005698648687
42	22.859057183964794	22.488221852342935	28.963805117499362	25.688915846192906
43	22.164005416537368	21.996572347448303	29.310026236352865	26.529395999661464
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	125.0
1	134.0
2	143.0
3	261.0
4	379.0
5	379.0
6	516.0
7	653.0
8	669.5
9	686.0
10	942.0
11	1198.0
12	1198.0
13	2081.5
14	2965.0
15	4341.5
16	5718.0
17	5639.0
18	5560.0
19	5560.0
20	6598.0
21	7636.0
22	7441.0
23	7246.0
24	8703.5
25	10161.0
26	10161.0
27	12454.5
28	14748.0
29	18162.5
30	21577.0
31	25391.0
32	29205.0
33	29205.0
34	35088.0
35	40971.0
36	47076.5
37	53182.0
38	59528.0
39	65874.0
40	65874.0
41	72307.0
42	78740.0
43	83333.0
44	87926.0
45	92386.5
46	96847.0
47	96847.0
48	100448.0
49	104049.0
50	105997.5
51	107946.0
52	109415.0
53	110884.0
54	110884.0
55	108629.5
56	106375.0
57	102964.0
58	99553.0
59	95398.0
60	91243.0
61	91243.0
62	83290.5
63	75338.0
64	67142.0
65	58946.0
66	51467.5
67	43989.0
68	43989.0
69	37930.5
70	31872.0
71	26995.0
72	22118.0
73	17688.5
74	13259.0
75	13259.0
76	10665.0
77	8071.0
78	6563.5
79	5056.0
80	4230.0
81	3404.0
82	3404.0
83	2623.5
84	1843.0
85	1540.0
86	1237.0
87	988.0
88	739.0
89	739.0
90	538.5
91	338.0
92	197.0
93	56.0
94	38.5
95	21.0
96	21.0
97	11.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1417880.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.6361018445801
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.21318563444798	60.54609193240822
2	6.756670038667469	9.275029858084066
3	1.9112055320958128	3.935330926405593
4	0.9202307321295576	2.526442010038273
5	0.5029827749481467	1.7261388483705256
6	0.3286451913015942	1.3534154892544639
7	0.2341276751929785	1.1248727671426029
8	0.15364325338242324	0.8436379189510903
9	0.1294591648393646	0.799701518036602
>10	0.75110042878432	9.766697935437026
>50	0.061874373517810485	2.9103161507379616
>100	0.03594819079462748	4.440331934139374
>500	6.180065986106212E-4	0.2783299147489071
>1k	3.090032993053106E-4	0.4736627962452826
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2545	0.17949332806725535	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2375	0.16750359691934436	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1780	0.125539537901656	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	7.052783028182921E-5	0.0	7.052783028182921E-5	0.0
6	0.0	7.052783028182921E-5	0.0	7.052783028182921E-5	0.0
7	0.0	7.052783028182921E-5	0.0	7.052783028182921E-5	0.0
8	0.0	7.052783028182921E-5	0.0	7.052783028182921E-5	1.4105566056365842E-4
9	0.0	7.052783028182921E-5	0.0	1.4105566056365842E-4	1.4105566056365842E-4
10	0.0	7.052783028182921E-5	0.0	1.4105566056365842E-4	1.4105566056365842E-4
11	0.0	7.052783028182921E-5	0.0	2.8211132112731683E-4	1.4105566056365842E-4
12	0.0	7.052783028182921E-5	0.0	2.8211132112731683E-4	1.4105566056365842E-4
13	0.0	7.052783028182921E-5	0.0	2.8211132112731683E-4	1.4105566056365842E-4
14	0.0	1.4105566056365842E-4	0.0	2.8211132112731683E-4	1.4105566056365842E-4
15	0.0	1.4105566056365842E-4	0.0	2.8211132112731683E-4	1.4105566056365842E-4
16	0.0	1.4105566056365842E-4	0.0	5.642226422546337E-4	1.4105566056365842E-4
17	0.0	1.4105566056365842E-4	0.0	7.052783028182921E-4	1.4105566056365842E-4
18	0.0	1.4105566056365842E-4	0.0	8.463339633819505E-4	1.4105566056365842E-4
19	0.0	1.4105566056365842E-4	0.0	9.87389623945609E-4	1.4105566056365842E-4
20	0.0	1.4105566056365842E-4	0.0	0.0011989731147910966	1.4105566056365842E-4
21	0.0	1.4105566056365842E-4	0.0	0.0012695009450729257	2.1158349084548762E-4
22	0.0	1.4105566056365842E-4	0.0	0.001974779247891218	2.1158349084548762E-4
23	0.0	1.4105566056365842E-4	0.0	0.002609529720427681	2.1158349084548762E-4
24	0.0	1.4105566056365842E-4	0.0	0.004725364628882557	2.1158349084548762E-4
25	0.0	1.4105566056365842E-4	0.0	0.006559088216210117	2.1158349084548762E-4
26	0.0	1.4105566056365842E-4	0.0	0.011989731147910966	2.1158349084548762E-4
27	0.0	1.4105566056365842E-4	0.0	0.03469969249865997	2.1158349084548762E-4
28	0.0	1.4105566056365842E-4	0.0	0.11919203317629136	2.1158349084548762E-4
29	0.0	2.1158349084548762E-4	0.0	0.22660591869551724	2.1158349084548762E-4
30	0.0	2.1158349084548762E-4	0.0	0.373726972663413	2.1158349084548762E-4
31	0.0	2.1158349084548762E-4	0.0	0.8314525911924846	2.1158349084548762E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1005	0.0	25.034824	1
GTACGGA	100	5.8797395E-6	16.65	6
GTATCAA	1530	0.0	16.202614	2
CTTATAC	1475	0.0	15.928814	37
TCTTATA	2475	0.0	15.248486	37
GTGCTAC	135	3.973746E-7	15.074073	1
TATACTG	135	3.973746E-7	15.074073	5
ACCGTCG	165	1.6456397E-8	14.575757	8
TACGGAT	90	8.275692E-4	14.388889	7
ATACTGC	145	8.913412E-7	14.034483	6
GTATTAT	120	3.3014287E-5	13.874999	1
CATACTG	120	3.3014287E-5	13.874999	5
AGCGTCA	190	7.1268005E-9	13.631579	3
GCGGTAT	240	8.0035534E-11	13.104166	24
GTTACAC	85	0.0094061475	13.058823	3
CTCTTAT	3680	0.0	12.769022	37
TTATACA	815	0.0	12.711657	37
TATAGTG	205	2.0656444E-8	12.634146	5
TAGACGT	105	0.0026440944	12.333333	4
ACCGACC	105	0.0026440944	12.333333	8
>>END_MODULE