FastQCFastQC Report
Fri 10 Feb 2017
ERR1632000.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1632000.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78703
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT880.11181276444354091No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT790.10037736807999695TruSeq Adapter, Index 5 (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT790.10037736807999695No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTA352.3603607E-531.7142879
GAGAGCA250.005465672429.624
GGTATCA556.138089E-726.909091
TCTTATA508.9514215E-625.90000237
GAGTATT400.001915333923.12511
TGAGTAT400.001915333923.12510
GAAAGCT400.001915333923.1254
GCTGAGT603.6698402E-521.5833348
GTATCAA603.6698402E-521.5833342
ATCTCGT450.003794699420.55555727
TCCGAGC450.003794699420.5555573
CACGAGA450.003794699420.55555711
TCTCCGA450.003794699420.5555571
CGAGACA450.003794699420.55555713
GAACAGA450.003794699420.5555576
TGAGCGA450.003794699420.55555721
CGTATGC500.00697822918.531
CTCGTAT500.00697822918.529
GACACTG500.00697822918.516
CTGAGCG500.00697822918.520
Produced by FastQC (version 0.11.5)